Restriction map for
pFN21AE2625
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BsaI BsiWI
BsmI BspDI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HindIII HpaI KasI KpnI MamI
MluI MluNI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 175 GACGTC GACGT^C
AccII 15 CGCG CG^CG
AcyI 175 GACGTC G[GA]^CG[CT]C
AflIII 251 ACGTGT A^C[GA][CT]GT
Asp700I 1225 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 437 GTGCTC G[AT]GC[AT]^C
AvaI 27 CCCGGG C^[CT]CG[GA]G
AvaII 1255 GGACC G^G[AT]CC
BamHI 1029 GGATCC G^GATCC
BanI 1185 GGTGCC G^G[CT][GA]CC
BbsI 396 GAAGAC GAAGAC(2/6)
BglII 278 AGATCT A^GATCT
BlpI 48 GCTCAGC GC^T[ACGT]AGC
BmyI 437 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC
BpuAI 396 GAAGAC GAAGAC(2/6)
BsaHI 175 GACGTC G[GA]^CG[CT]C
BsiEI 1148 CGACCG CG[GA][CT]^CG
BsiHKAI 437 GTGCTC G[AT]GC[AT]^C
BsmAI 760 GAGAC GTCTC(1/5)
BsmBI 760 GAGACG CGTCTC(1/5)
BsoBI 27 CCCGGG C^[CT]CG[GA]G
Bsp1286I 437 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 1019 TGTACA T^GTACA
BsrDI 161 GCAATG GCAATG(2/0)
BsrFI 326 ACCGGC [GA]^CCGG[CT]
BsrGI 1019 TGTACA T^GTACA
BstAPI 330 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 789 GGTCACC G^GT[ACGT]ACC
BstPI 789 GGTCACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
BstXI 203 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 48 GCTCAGC GC^T[ACGT]AGC
Cfr10I 326 ACCGGC [GA]^CCGG[CT]
Cfr13I 1255 GGACC G^G[ACGT]CC
DrdI 1256 GACCAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 789 GGTCACC G^GT[ACGT]ACC
EcoT22I 892 ATGCAT ATGCA^T
Hin1I 175 GACGTC G[GA]^CG[CT]C
HincII 800 GTCGAC GT[CT]^[GA]AC
HindII 800 GTCGAC GT[CT]^[GA]AC
KspI 14 CCGCGG CCGC^GG
MfeI 89 CAATTG C^AATTG
MslI 630 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 89 CAATTG C^AATTG
MvnI 15 CGCG CG^CG
NsiI 892 ATGCAT ATGCA^T
SacII 14 CCGCGG CCGC^GG
SalI 800 GTCGAC G^TCGAC
SapI 808 GAAGAGC GCTCTTC(1/4)
Sau96I 1255 GGACC G^G[ACGT]CC
SmaI 27 CCCGGG CCC^GGG
SspBI 1019 TGTACA T^GTACA
TfiI 727 GATTC G^A[AT]TC
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
XmnI 1225 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 178 GTCTAC GT^[AC][GT]AC
800 GTCGAC
AccIII 674 TCCGGA T^CCGGA
934 TCCGGA
AocI 587 CCTCAGG CC^T[ACGT]AGG
1033 CCTCAGG
BsaWI 674 TCCGGA [AT]^CCGG[AT]
934 TCCGGA
BseAI 674 TCCGGA T^CCGGA
934 TCCGGA
BseRI 1043 GAGGAG GAGGAG(10/8)
1220 GAGGAG
BsmFI 1254 GGGAC GGGAC(10/14)
646 GTCCC
BspEI 674 TCCGGA T^CCGGA
934 TCCGGA
BstYI 278 AGATCT [GA]^GATC[CT]
1029 GGATCC
Bsu36I 587 CCTCAGG CC^T[ACGT]AGG
1033 CCTCAGG
Cac8I 919 GCACGC GC[ACGT]^[ACGT]GC
1073 GCTTGC
Eco81I 587 CCTCAGG CC^T[ACGT]AGG
1033 CCTCAGG
FauI 32 GCGGG CCCGC(4/6)
1208 GCGGG
HphI 575 TCACC GGTGA(8/7)
791 TCACC
MaeII 176 ACGT A^CGT
251 ACGT
MflI 278 AGATCT [GA]^GATC[CT]
1029 GGATCC
MroI 674 TCCGGA T^CCGGA
934 TCCGGA
MseI 377 TTAA T^TAA
1057 TTAA
PleI 40 GAGTC GAGTC(4/5)
355 GAGTC
PvuII 51 CAGCTG CAG^CTG
139 CAGCTG
TaqI 801 TCGA T^CGA
817 TCGA
Tru9I 377 TTAA T^TAA
1057 TTAA
TthHB8I 801 TCGA T^CGA
817 TCGA
XhoII 278 AGATCT [GA]^GATC[CT]
1029 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BfaI BpmI BsaJI BsgI
BsiYI BslI BsrI BssKI BstF5I
BstNI CfoI DdeI DpnII DsaI
EarI EcoRII Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI
XcmI