Restriction map for
pFN21AE2625
(Circular) (Six-base) MAPSORT of: pFN21AE2625.seq Check: 5371 from: 1 to: 6043
pFN21AE2625 6043 bp
With 182 enzymes: SgfI *
May 20, 2016 17:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2184 3853 278
Size: 53 83 186 936 648 1669 2468
Fragments arranged by size:
2468 1669 936 648 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2184 2806 3386 1937
Size: 247 622 580 4594
Fragments arranged by size:
4594 622 580 247
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2481 3162 4106 5490 5758 1632
Size: 849 681 944 1384 268 1917
Fragments arranged by size:
1917 1384 944 849 681 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 2256 4942 5919 1203
Size: 1053 2686 977 1327
Fragments arranged by size:
2686 1327 1053 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 3786 5358 3786
Size: 1572 4471
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 3368 1870
Size: 73 89 1336 4545
Fragments arranged by size:
4545 1336 89 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2941 2974 2941
Size: 33 6010
BamHI G'GATC_C
Cuts at: 3034 3373 3740 3034
Size: 339 367 5337
Fragments arranged by size:
5337 367 339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3190 3364 4819 618
Size: 325 217 627 1403 174 1455 1842
Fragments arranged by size:
1842 1455 1403 627 325 217 174
BanII G_rGCy'C
Cuts at: 1391 3362 1391
Size: 1971 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2408 928
Size: 971 509 4563
Fragments arranged by size:
4563 971 509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BfrBI ATG'CAT
Cuts at: 2899 2899
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4654 136
Size: 107 122 71 631 605 337 2645 1525
Fragments arranged by size:
2645 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2283 6038 2283
Size: 3755 2288
BlpI GC'TnA_GC
Cuts at: 2054 3460 2054
Size: 1406 4637
Bme1580I G_kGCm'C
Cuts at: 1790 4104 5260 1790
Size: 2314 1156 2573
Fragments arranged by size:
2573 2314 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3774 4732 442
Size: 416 53 83 399 24 2357 958 1753
Fragments arranged by size:
2357 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2191 2287 2737 2848 4703 1239
Size: 952 96 450 111 1855 2579
Fragments arranged by size:
2579 1855 952 450 111 96
Bpu10I CC'TnA_GC
Cuts at: 3088 3088
Size: 6043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4706 882
Size: 617 3207 2219
Fragments arranged by size:
3207 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 3850 4232 4867
Size: 53 83 186 936 648 1669 382 635
Cuts at: 4867 275
Size: 1451
Fragments arranged by size:
1669 1451 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2679 2939 4476 4874 5148 5295 5706 2679
Size: 260 1537 398 274 147 411 3016
Fragments arranged by size:
3016 1537 411 398 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 3042 3072 1178
Size: 30 165 30 882 30 727 30 4149
Fragments arranged by size:
4149 882 727 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3063 3240 1186
Size: 1877 177 3989
Fragments arranged by size:
3989 1877 177
BseYI C'CCAG_C
Cuts at: 5246 5742 5246
Size: 496 5547
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2527 2907 1163
Size: 573 329 462 380 4299
Fragments arranged by size:
4299 573 462 380 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3156 3493 4254 5282 1540
Size: 275 186 1155 337 761 1028 2301
Fragments arranged by size:
2301 1155 1028 761 337 275 186
BsiHKAI G_wGCw'C
Cuts at: 2446 4104 4189 5260 2446
Size: 1658 85 1071 3229
Fragments arranged by size:
3229 1658 1071 85
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
BsmBI CGTCTCn'nnnn_
Cuts at: 2759 2759
Size: 6043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 3362 4104 4189 5260 1391
Size: 399 656 916 742 85 1071 2174
Fragments arranged by size:
2174 1071 916 742 656 399 85
BspEI T'CCGG_A
Cuts at: 2679 2939 5706 2679
Size: 260 2767 3016
Fragments arranged by size:
3016 2767 260
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3398 844
Size: 2554 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2173 4538 4712 65
Size: 1183 925 2365 174 1396
Fragments arranged by size:
2365 1396 1183 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2331 4687 4874 1700
Size: 110 521 2356 187 2869
Fragments arranged by size:
2869 2356 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 3024 96
Size: 2928 3115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 794
Size: 1547 4496
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 2794 1184
Size: 1610 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2215
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2283 3034 3373 3740 4125 4142 5594 5680
Size: 311 751 339 367 385 17 1452 86
Cuts at: 5680 5692 6038 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 751 385 367 346 339 311
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2593 3039 2593
Size: 446 5597
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 2158 2243 2984 3186 3310
Size: 552 939 15 139 85 741 202 124
Cuts at: 3310 513
Size: 3246
Fragments arranged by size:
3246 939 741 552 202 139 124 85
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 2919 3559 4353 4373 2919
Size: 640 794 20 4589
Fragments arranged by size:
4589 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3267 5050 809
Size: 507 1951 1783 1802
Fragments arranged by size:
1951 1802 1783 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008
Size: 54 1425 50 275 18 1660 889 1629
Cuts at: 6008 8
Size: 43
Fragments arranged by size:
1660 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2352 2808 3096 3973 1353
Size: 854 145 456 288 877 3423
Fragments arranged by size:
3423 877 854 456 288 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2287 2481 2737 2848 3162 4106
Size: 393 559 96 194 256 111 314 944
Cuts at: 4106 4703 5490 5758 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 944 787 597 559 393 314 268
256 194 111 96
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2511 3192 2511
Size: 681 5362
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2054 2200 3221 5190 1678
Size: 376 146 1021 1969 2531
Fragments arranged by size:
2531 1969 1021 376 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2510 2542 3042
Size: 32 135 32 497 32 409 32 500
Cuts at: 3042 3074 4684 4716 4758 4790 1373
Size: 32 1610 32 42 32 2626
Fragments arranged by size:
2626 1610 500 497 409 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2807 3387 3633 669
Size: 988 281 869 580 246 3079
Fragments arranged by size:
3079 988 869 580 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 2094 3642 2094
Size: 1548 4495
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5157 5341 1305
Size: 3852 184 2007
Fragments arranged by size:
3852 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 4001 4360 4519 518
Size: 648 1473 1362 359 159 2042
Fragments arranged by size:
2042 1473 1362 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2058 2146 3459 4136 5284 5529 1606
Size: 415 37 88 1313 677 1148 245 2120
Fragments arranged by size:
2120 1313 1148 677 415 245 88 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NsiI A_TGCA'T
Cuts at: 2901 2901
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6043
PfoI T'CCnGG_A
Cuts at: 2175 2409 2482 2175
Size: 234 73 5736
Fragments arranged by size:
5736 234 73
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 3395 830
Size: 2565 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2058 2146 2058
Size: 88 5955
SacII CC_GC'GG
Cuts at: 2022 2022
Size: 6043
SalI G'TCGA_C
Cuts at: 1936 2805 3385 1936
Size: 869 580 4594
Fragments arranged by size:
4594 869 580
SapI GCTCTTCn'nnn_
Cuts at: 2808 2808
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 3391 4526 5207 5398 826
Size: 219 2346 1135 681 191 1471
Fragments arranged by size:
2346 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 2034 3370 1872
Size: 162 1336 4545
Fragments arranged by size:
4545 1336 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6043
StyI C'CwwG_G
Cuts at: 513 1065 2004 3482 513
Size: 552 939 1478 3074
Fragments arranged by size:
3074 1478 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4070 4087 4240 4425 4870 325
Size: 1255 2490 17 153 185 445 1498
Fragments arranged by size:
2490 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3024 4289 6001
Size: 274 80 33 51 1443 1047 1265 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1712 1443 1265 1047 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6003
Size: 32 6011
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2470 3080 2215
Size: 255 610 5178
Fragments arranged by size:
5178 610 255
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 2032 3368 1870
Size: 162 1336 4545
Fragments arranged by size:
4545 1336 162
XmnI GAAnn'nnTTC
Cuts at: 3234 4172 3234
Size: 938 5105
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2182 3851 276
Size: 53 83 186 936 648 1669 2468
Fragments arranged by size:
2468 1669 936 648 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI