(Linear) (Six-base) MAPSORT of: KIEE2625.seq Check: 9500 from: 1 to: 1338 KIEE2625.orf With 182 enzymes: SgfI * May 20, 2016 17:05 .. AatII G_ACGT'C Cuts at: 0 179 1338 Size: 179 1159 AccI GT'mk_AC Cuts at: 0 179 801 1338 Size: 179 622 537 Fragments arranged by size: 622 537 179 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 476 1157 1338 Size: 476 681 181 Fragments arranged by size: 681 476 181 AflIII A'CryG_T Cuts at: 0 251 1338 Size: 251 1087 AvaI C'yCGr_G Cuts at: 0 27 1338 Size: 27 1311 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 936 969 1338 Size: 936 33 369 Fragments arranged by size: 936 369 33 BamHI G'GATC_C Cuts at: 0 1029 1338 Size: 1029 309 BanI G'GyrC_C Cuts at: 0 1185 1338 Size: 1185 153 BbsI GAAGACnn'nnnn_ Cuts at: 0 403 1338 Size: 403 935 BfrBI ATG'CAT Cuts at: 0 894 1338 Size: 894 444 BglII A'GATC_T Cuts at: 0 278 1338 Size: 278 1060 BlpI GC'TnA_GC Cuts at: 0 49 1338 Size: 49 1289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 282 732 843 1338 Size: 186 96 450 111 495 Fragments arranged by size: 495 450 186 111 96 Bpu10I CC'TnA_GC Cuts at: 0 1083 1338 Size: 1083 255 BsaHI Gr'CG_yC Cuts at: 0 176 1338 Size: 176 1162 BsaWI w'CCGG_w Cuts at: 0 674 934 1338 Size: 674 260 404 Fragments arranged by size: 674 404 260 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 280 310 1037 1067 1338 Size: 280 30 727 30 271 Fragments arranged by size: 727 280 271 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1058 1235 1338 Size: 1058 177 103 Fragments arranged by size: 1058 177 103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 522 902 1338 Size: 60 462 380 436 Fragments arranged by size: 462 436 380 60 BsiEI CG_ry'CG Cuts at: 0 1151 1338 Size: 1151 187 BsiHKAI G_wGCw'C Cuts at: 0 441 1338 Size: 441 897 BsmBI CGTCTCn'nnnn_ Cuts at: 0 754 1338 Size: 754 584 Bsp1286I G_dGCh'C Cuts at: 0 441 1338 Size: 441 897 BspEI T'CCGG_A Cuts at: 0 674 934 1338 Size: 674 260 404 Fragments arranged by size: 674 404 260 BsrDI GCAATG_nn' Cuts at: 0 168 1338 Size: 168 1170 BsrFI r'CCGG_y Cuts at: 0 326 1338 Size: 326 1012 BsrGI T'GTAC_A Cuts at: 0 1019 1338 Size: 1019 319 BstAPI GCAn_nnn'nTGC Cuts at: 0 336 1338 Size: 336 1002 BstEII G'GTnAC_C Cuts at: 0 789 1338 Size: 789 549 BstXI CCAn_nnnn'nTGG Cuts at: 0 210 1338 Size: 210 1128 BstYI r'GATC_y Cuts at: 0 278 1029 1338 Size: 278 751 309 Fragments arranged by size: 751 309 278 Bsu36I CC'TnA_GG Cuts at: 0 588 1034 1338 Size: 588 446 304 Fragments arranged by size: 588 446 304 BtgI C'CryG_G Cuts at: 0 14 153 238 979 1181 1305 1338 Size: 14 139 85 741 202 124 33 Fragments arranged by size: 741 202 139 124 85 33 14 BtsI GCAGTG_nn' Cuts at: 0 914 1338 Size: 914 424 DrdI GACnn_nn'nnGTC Cuts at: 0 1262 1338 Size: 1262 76 EarI CTCTTCn'nnn_ Cuts at: 0 202 347 803 1091 1338 Size: 202 145 456 288 247 Fragments arranged by size: 456 288 247 202 145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 282 476 732 843 1157 1338 Size: 186 96 194 256 111 314 181 Fragments arranged by size: 314 256 194 186 181 111 96 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 506 1187 1338 Size: 506 681 151 Fragments arranged by size: 681 506 151 HaeII r_GCGC'y Cuts at: 0 49 195 1216 1338 Size: 49 146 1021 122 Fragments arranged by size: 1021 146 122 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 505 537 1037 1069 1338 Size: 64 32 409 32 500 32 269 Fragments arranged by size: 500 409 269 64 32 32 32 HincII GTy'rAC Cuts at: 0 802 1338 Size: 802 536 MfeI C'AATT_G Cuts at: 0 89 1338 Size: 89 1249 MslI CAynn'nnrTG Cuts at: 0 634 1338 Size: 634 704 MspA1I CmG'CkG Cuts at: 0 16 53 141 1338 Size: 16 37 88 1197 Fragments arranged by size: 1197 88 37 16 NsiI A_TGCA'T Cuts at: 0 896 1338 Size: 896 442 PfoI T'CCnGG_A Cuts at: 0 170 404 477 1338 Size: 170 234 73 861 Fragments arranged by size: 861 234 170 73 PvuII CAG'CTG Cuts at: 0 53 141 1338 Size: 53 88 1197 Fragments arranged by size: 1197 88 53 SacII CC_GC'GG Cuts at: 0 17 1338 Size: 17 1321 SalI G'TCGA_C Cuts at: 0 800 1338 Size: 800 538 SapI GCTCTTCn'nnn_ Cuts at: 0 803 1338 Size: 803 535 SmaI CCC'GGG Cuts at: 0 29 1338 Size: 29 1309 TatI w'GTAC_w Cuts at: 0 1019 1338 Size: 1019 319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 210 465 1075 1338 Size: 210 255 610 263 Fragments arranged by size: 610 263 255 210 XmaI C'CCGG_G Cuts at: 0 27 1338 Size: 27 1311 XmnI GAAnn'nnTTC Cuts at: 0 1229 1338 Size: 1229 109 ZraI GAC'GTC Cuts at: 0 177 1338 Size: 177 1161 Enzymes that do cut: AatII AccI AcuI AflIII AvaI BaeI BamHI BanI BbsI BfrBI BglII BlpI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EarI Eco57MI FalI HaeII Hin4I HincII MfeI MslI MspA1I NsiI PfoI PvuII SacII SalI SapI SmaI TatI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BanII BbeI BbvCI BcgI BciVI BclI BglI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BseYI BsiWI BsmI BspHI BspMI BsrBI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI