################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AclI 101 AACGTT AA^CGTT AcyI 848 GGCGCC G[GA]^CG[CT]C AlwNI 386 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 190 CCTGAGG CC^T[ACGT]AGG Asp700I 382 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 145 GGACC G^G[AT]CC BanI 848 GGCGCC G^G[CT][GA]CC BbeI 848 GGCGCC GGCGC^C BbvI 260 GCAGC GCAGC(8/12) BsaHI 848 GGCGCC G[GA]^CG[CT]C BsaJI 1019 CCGTGG C^C[ACGT][ACGT]GG BsiYI 186 CCACCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 186 CCACCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 542 GAGAC GTCTC(1/5) BsmFI 458 GGGAC GGGAC(10/14) BsmI 159 GCATTC GAATGC(1/-1) BsrFI 845 ACCGGC [GA]^CCGG[CT] BstAPI 879 GCACAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 462 CATCC GGATG(2/0) Bsu36I 190 CCTGAGG CC^T[ACGT]AGG CfoI 849 GCGC GCG^C Cfr10I 845 ACCGGC [GA]^CCGG[CT] Cfr13I 145 GGACC G^G[ACGT]CC DdeI 191 CTGAG C^T[ACGT]AG DsaI 1019 CCGTGG C^C[GA][CT]GG Eco81I 190 CCTGAGG CC^T[ACGT]AGG Fnu4HI 260 GCAGC GC^[ACGT]GC FokI 462 CATCC GGATG(9/13) HaeII 848 GGCGCC [GA]GCGC^[CT] HaeIII 570 GGCC GG^CC HapII 846 CCGG C^CGG HhaI 849 GCGC GCG^C Hin1I 848 GGCGCC G[GA]^CG[CT]C HinP1I 849 GCGC G^CGC HpaII 846 CCGG C^CGG ItaI 260 GCAGC GC^[ACGT]GC KasI 848 GGCGCC G^GCGCC MaeII 102 ACGT A^CGT MspI 846 CCGG C^CGG MwoI 879 GCACAAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 848 GGCGCC GG^CGCC Psp1406I 101 AACGTT AA^CGTT Sau96I 145 GGACC G^G[ACGT]CC SfoI 848 GGCGCC GGC^GCC SpeI 721 ACTAGT A^CTAGT StuI 569 AGGCCT AGG^CCT TfiI 952 GAATC G^A[AT]TC TseI 260 GCAGC G^C[AT]GC XcmI 538 CCAAGAGACTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 382 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BpmI 977 CTCCAG CTGGAG(16/14) 968 CTGGAG BssSI 991 CACGAG CACGAG(-5/-1) 546 CTCGTG BstXI 166 CCATCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 824 CCAACCACTTGG HphI 933 GGTGA GGTGA(8/7) 179 TCACC NspI 632 GCATGC [GA]CATG^[CT] 805 GCATGC PleI 544 GACTC GAGTC(4/5) 419 GAGTC SfaNI 88 GATGC GCATC(5/9) 496 GATGC SmlI 363 CTTGAG C^T[CT][GA]AG 661 CTCAAG SphI 632 GCATGC GCATG^C 805 GCATGC TaqI 695 TCGA T^CGA 738 TCGA Tsp45I 680 GTGAC ^GT[GC]AC 989 GTCAC TthHB8I 695 TCGA T^CGA 738 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AcsI AluI AlwI ApoI BfaI BsrI BssKI BstNI Cac8I DpnII EcoRII HinfI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI Tru9I Tsp509I TspRI