################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BcnI BfaI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 101 GGTACC G^GTACC AccI 394 GTCTAC GT^[AC][GT]AC AccII 131 CGCG CG^CG AgeI 98 ACCGGT A^CCGGT Asp718I 101 GGTACC G^GTACC AspHI 670 GAGCAC G[AT]GC[AT]^C AvaII 96 GGACC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanI 101 GGTACC G^G[CT][GA]CC BanII 144 GAGCCC G[GA]GC[CT]^C BcgI 82 GCAGCCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 503 TGATCA T^GATCA BglI 4 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 67 GCTGAGC GC^T[ACGT]AGC Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC BsiHKAI 670 GAGCAC G[AT]GC[AT]^C BsmAI 18 GTCTC GTCTC(1/5) BsmFI 294 GTCCC GGGAC(10/14) BspHI 485 TCATGA T^CATGA BsrDI 215 GCAATG GCAATG(2/0) BsrFI 98 ACCGGT [GA]^CCGG[CT] BstUI 131 CGCG CG^CG BstYI 134 GGATCT [GA]^GATC[CT] CelII 67 GCTGAGC GC^T[ACGT]AGC CfoI 206 GCGC GCG^C Cfr10I 98 ACCGGT [GA]^CCGG[CT] DraII 590 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EcoO109I 590 AGGGCCT [GA]G^G[ACGT]CC[CT] FbaI 503 TGATCA T^GATCA HaeII 205 AGCGCC [GA]GCGC^[CT] HhaI 206 GCGC GCG^C HinP1I 206 GCGC G^CGC KpnI 101 GGTACC GGTAC^C MaeII 150 ACGT A^CGT MaeIII 631 GTGAC ^GT[ACGT]AC MflI 134 GGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 516 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 131 CGCG CG^CG NlaIV 101 GGTACC GG[ACGT]^[ACGT]CC PinAI 98 ACCGGT A^CCGGT RcaI 485 TCATGA T^CATGA SfcI 662 CTGTAG C^T[GA][CT]AG Tsp45I 631 GTGAC ^GT[GC]AC XcmI 29 CCAGCCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 134 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 76 GTAC GT^AC 102 GTAC AlwI 489 GATCC GGATC(4/5) 134 GGATC BsrI 35 ACTGG ACTGG(1/-1) 73 CCAGT DdeI 63 CTCAG C^T[ACGT]AG 68 CTGAG EarI 511 CTCTTC CTCTTC(1/4) 289 GAAGAG EcoT14I 327 CCAAGG C^C[AT][AT]GG 440 CCTTGG HgaI 495 GACGC GACGC(5/10) 633 GACGC Ksp632I 511 CTCTTC CTCTTC(1/4) 289 GAAGAG PflMI 29 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 33 CCACTGGATGG PleI 292 GAGTC GAGTC(4/5) 422 GAGTC RsaI 76 GTAC GT^AC 102 GTAC SfaNI 332 GCATC GCATC(5/9) 353 GCATC StyI 327 CCAAGG C^C[AT][AT]GG 440 CCTTGG TspRI 34 CACTG CA[GC]TG[ACGT][ACGT]^ 190 CACTG Van91I 29 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 33 CCACTGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI AlwNI BbvI BmyI BsaJI BsaWI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII PvuII Sau3AI Sau96I ScrFI TfiI TseI