(Circular) (Six-base) MAPSORT of: pFN21AE6685.seq Check: 4243 from: 1 to: 5392 pFN21AE6685 5392 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3202 278 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2400 2735 1937 Size: 463 335 4594 Fragments arranged by size: 4594 463 335 Acc65I G'GTAC_C Cuts at: 2106 2713 2106 Size: 607 4785 AclI AA'CG_TT Cuts at: 1563 3519 3892 1563 Size: 1956 373 3063 Fragments arranged by size: 3063 1956 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 2477 2495 3455 4839 5107 1632 Size: 659 186 18 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 659 268 186 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5195 AflIII A'CryG_T Cuts at: 1203 4291 5268 1203 Size: 3088 977 1327 Fragments arranged by size: 3088 1327 977 AgeI A'CCGG_T Cuts at: 2103 4223 2103 Size: 2120 3272 AhdI GACnn_n'nnGTC Cuts at: 4121 4121 Size: 5392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2438 1184 Size: 1254 4138 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 2198 Size: 32 5360 AlwNI CAG_nnn'CTG Cuts at: 2040 2269 2503 3135 4707 2040 Size: 229 234 632 1572 2725 Fragments arranged by size: 2725 1572 632 234 229 ApaLI G'TGCA_C Cuts at: 3449 4605 3449 Size: 1156 4236 ApoI r'AATT_y Cuts at: 1493 2701 2927 3079 5188 1493 Size: 1208 226 152 2109 1697 Fragments arranged by size: 2109 1697 1208 226 152 AseI AT'TA_AT Cuts at: 160 1032 3946 160 Size: 872 2914 1606 Fragments arranged by size: 2914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 AvaI C'yCGr_G Cuts at: 1870 1943 2717 1870 Size: 73 774 4545 Fragments arranged by size: 4545 774 73 BamHI G'GATC_C Cuts at: 2722 3089 2722 Size: 367 5025 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2713 4168 618 Size: 325 217 627 319 607 1455 1842 Fragments arranged by size: 1842 1455 627 607 325 319 217 BanII G_rGCy'C Cuts at: 1391 2153 2711 1391 Size: 762 558 4072 Fragments arranged by size: 4072 762 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2076 2110 3583 3617 1903 Size: 34 139 34 1473 34 3678 Fragments arranged by size: 3678 1473 139 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3284 4494 3284 Size: 1210 4182 BclI T'GATC_A Cuts at: 1548 2508 1548 Size: 960 4432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 4003 136 Size: 107 122 71 631 605 343 1988 1525 Fragments arranged by size: 1988 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5387 5387 Size: 5392 BlpI GC'TnA_GC Cuts at: 2073 2809 2073 Size: 736 4656 Bme1580I G_kGCm'C Cuts at: 1790 2034 3453 4609 1790 Size: 244 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 244 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5392 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3123 4081 442 Size: 416 53 83 399 24 1706 958 1753 Fragments arranged by size: 1753 1706 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5392 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4052 1239 Size: 2813 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3517 4382 4680 3517 Size: 865 298 4229 Fragments arranged by size: 4229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4055 882 Size: 617 2556 2219 Fragments arranged by size: 2556 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3183 493 Size: 711 1979 2702 Fragments arranged by size: 2702 1979 711 BsaBI GATnn'nnATC Cuts at: 5386 5386 Size: 5392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3199 3581 4216 275 Size: 53 83 186 936 1666 382 635 1451 Fragments arranged by size: 1666 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2103 2109 2340 3825 4223 4497 4644 5055 2103 Size: 6 231 1485 398 274 147 411 2440 Fragments arranged by size: 2440 1485 411 398 274 231 147 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2198 2228 1178 Size: 30 165 30 687 30 78 30 4342 Fragments arranged by size: 4342 687 165 78 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5392 BseYI C'CCAG_C Cuts at: 2033 2262 4595 5091 2033 Size: 229 2333 496 2334 Fragments arranged by size: 2334 2333 496 229 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4819 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2842 3603 4631 1540 Size: 275 186 841 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2679 3453 3538 4609 2679 Size: 774 85 1071 3462 Fragments arranged by size: 3462 1071 774 85 BsmI GAATG_Cn' Cuts at: 2903 2996 2903 Size: 93 5299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2153 2679 2711 3453 3538 4609 Size: 399 244 119 526 32 742 85 1071 Cuts at: 4609 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 526 399 244 119 85 32 BspEI T'CCGG_A Cuts at: 5055 5055 Size: 5392 BspHI T'CATG_A Cuts at: 2490 3281 5011 2490 Size: 791 1730 2871 Fragments arranged by size: 2871 1730 791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 844 Size: 1903 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3279 1394 Size: 429 1456 3507 Fragments arranged by size: 3507 1456 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2227 3887 4061 65 Size: 1183 979 1660 174 1396 Fragments arranged by size: 1660 1396 1183 979 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 4036 4223 1700 Size: 110 293 1933 187 2869 Fragments arranged by size: 2869 1933 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5392 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5392 BssSI C'ACGA_G Cuts at: 3452 4464 3452 Size: 1012 4380 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5392 BstBI TT'CG_AA Cuts at: 4196 4196 Size: 5392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5392 BstYI r'GATC_y Cuts at: 1972 2139 2722 3089 3474 3491 4943 5029 5041 Size: 167 583 367 385 17 1452 86 12 Cuts at: 5041 5387 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 583 385 367 346 167 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4653 Fragments arranged by size: 4653 683 56 BtsI GCAGTG_nn' Cuts at: 2194 2908 3702 3722 2194 Size: 714 794 20 3864 Fragments arranged by size: 3864 794 714 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5357 DraI TTT'AAA Cuts at: 2696 2696 Size: 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4399 809 Size: 507 3083 1802 Fragments arranged by size: 3083 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2839 3728 Size: 54 1425 50 275 18 177 832 889 Cuts at: 3728 5357 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 832 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 1537 Size: 275 1027 4090 Fragments arranged by size: 4090 1027 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 2522 3322 1353 Size: 936 233 800 3423 Fragments arranged by size: 3423 936 800 233 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3979 4353 4499 387 Size: 1064 138 2390 374 146 1280 Fragments arranged by size: 2390 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2477 2495 3455 4052 4839 5107 Size: 393 659 186 18 960 597 787 268 Cuts at: 5107 1239 Size: 1524 Fragments arranged by size: 1524 960 787 659 597 393 268 186 18 EcoO109I rG'GnC_Cy Cuts at: 2596 2596 Size: 5392 EcoRI G'AATT_C Cuts at: 2701 3079 2701 Size: 378 5014 FspI TGC'GCA Cuts at: 3898 3898 Size: 5392 HaeII r_GCGC'y Cuts at: 1678 2214 4539 1678 Size: 536 2325 2531 Fragments arranged by size: 2531 2325 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373 Size: 32 135 32 2461 32 42 32 2626 Fragments arranged by size: 2626 2461 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2982 669 Size: 988 281 798 246 3079 Fragments arranged by size: 3079 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5392 HpaI GTT'AAC Cuts at: 2982 2982 Size: 5392 KpnI G_GTAC'C Cuts at: 2110 2717 2110 Size: 607 4785 MfeI C'AATT_G Cuts at: 2991 2991 Size: 5392 MluI A'CGCG_T Cuts at: 5268 5268 Size: 5392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4506 4690 1305 Size: 3201 184 2007 Fragments arranged by size: 3201 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3393 Fragments arranged by size: 3393 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 3350 3709 3868 518 Size: 648 1359 825 359 159 2042 Fragments arranged by size: 2042 1359 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2072 2269 2455 2808 3485 4633 4878 1606 Size: 466 197 186 353 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 466 353 245 197 186 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3901 Fragments arranged by size: 3901 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5392 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5392 NotI GC'GGCC_GC Cuts at: 2839 2839 Size: 5392 NspI r_CATG'y Cuts at: 1132 1229 2750 4295 1132 Size: 97 1521 1545 2229 Fragments arranged by size: 2229 1545 1521 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5392 PciI A'CATG_T Cuts at: 4291 4291 Size: 5392 PflMI CCAn_nnn'nTGG Cuts at: 1108 2040 2044 1108 Size: 932 4 4456 Fragments arranged by size: 4456 932 4 PmeI GTTT'AAAC Cuts at: 2696 2696 Size: 5392 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2198 2230 3461 3493 4974 5006 2198 Size: 32 1231 32 1481 32 2584 Fragments arranged by size: 2584 1481 1231 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5392 PsiI TTA'TAA Cuts at: 2962 2962 Size: 5392 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2116 2148 2116 Size: 32 5360 PstI C_TGCA'G Cuts at: 830 2744 830 Size: 1914 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5392 PvuII CAG'CTG Cuts at: 2072 2269 2455 2072 Size: 197 186 5009 Fragments arranged by size: 5009 197 186 SalI G'TCGA_C Cuts at: 1936 2734 1936 Size: 798 4594 SbfI CC_TGCA'GG Cuts at: 2744 2744 Size: 5392 ScaI AGT'ACT Cuts at: 3640 5352 3640 Size: 1712 3680 SfcI C'TryA_G Cuts at: 826 1045 2667 2740 3875 4556 4747 826 Size: 219 1622 73 1135 681 191 1471 Fragments arranged by size: 1622 1471 1135 681 219 191 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5392 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5392 SmaI CCC'GGG Cuts at: 1872 2719 1872 Size: 847 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3496 4397 4659 820 Size: 197 926 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3183 493 Size: 2690 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5392 SphI G_CATG'C Cuts at: 1132 2750 1132 Size: 1618 3774 SspI AAT'ATT Cuts at: 5 52 3316 5 Size: 47 3264 2081 Fragments arranged by size: 3264 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5392 StyI C'CwwG_G Cuts at: 513 1065 2004 2332 2445 2831 513 Size: 552 939 328 113 386 3074 Fragments arranged by size: 3074 939 552 386 328 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2694 3419 3436 3589 3774 4219 325 Size: 1255 1114 725 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1114 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3638 5350 96 Size: 274 80 33 51 1443 1661 1712 138 Fragments arranged by size: 1712 1661 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 5352 5384 2645 Size: 32 2675 32 2653 Fragments arranged by size: 2675 2653 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5392 XbaI T'CTAG_A Cuts at: 2728 2728 Size: 5392 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2041 2011 Size: 30 5362 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5392 XmaI C'CCGG_G Cuts at: 1870 2717 1870 Size: 847 4545 XmnI GAAnn'nnTTC Cuts at: 3521 3521 Size: 5392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3200 276 Size: 53 83 186 936 1666 2468 Fragments arranged by size: 2468 1666 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI