Restriction map for
pFN21AE6618
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# NO CUT ENZYMES ( 142 enzymes) #
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Acc65I AccI AccIII AfaI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI MboI MfeI
MflI MluI MroI MunI NaeI
NarI NcoI NdeII NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI RcaI
RsaI RsrII SalI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 220 GACGTC GACGT^C
AclI 682 AACGTT AA^CGTT
AcsI 691 AAATTT [GA]^AATT[CT]
AcyI 220 GACGTC G[GA]^CG[CT]C
Alw44I 767 GTGCAC G^TGCAC
AlwNI 638 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 767 GTGCAC G^TGCAC
ApoI 691 AAATTT [GA]^AATT[CT]
BalI 314 TGGCCA TGG^CCA
BanI 127 GGTGCC G^G[CT][GA]CC
BbvCI 643 GCTGAGG CCTCAGC(-5/-2)
BglI 425 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 805 GCTGAGC GC^T[ACGT]AGC
BpmI 619 CTGGAG CTGGAG(16/14)
Bpu1102I 805 GCTGAGC GC^T[ACGT]AGC
BsaBI 624 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 220 GACGTC G[GA]^CG[CT]C
BsmAI 367 GAGAC GTCTC(1/5)
BsmFI 166 GTCCC GGGAC(10/14)
BsrBI 131 CCGCTC CCGCTC(-3/-3)
CelII 805 GCTGAGC GC^T[ACGT]AGC
CfoI 87 GCGC GCG^C
DraI 688 TTTAAA TTT^AAA
DraIII 718 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 158 CCGCGG C^C[GA][CT]GG
EaeI 314 TGGCCA [CT]^GGCC[GA]
EcoNI 816 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 392 CCAAGG C^C[AT][AT]GG
FauI 169 CCCGC CCCGC(4/6)
HgaI 144 GACGC GACGC(5/10)
HhaI 87 GCGC GCG^C
Hin1I 220 GACGTC G[GA]^CG[CT]C
HinP1I 87 GCGC G^CGC
HincII 357 GTCAAC GT[CT]^[GA]AC
HindII 357 GTCAAC GT[CT]^[GA]AC
HphI 561 GGTGA GGTGA(8/7)
KspI 158 CCGCGG CCGC^GG
MaeIII 539 GTCAC ^GT[ACGT]AC
MamI 624 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 314 TGGCCA TGG^CCA
MscI 314 TGGCCA TGG^CCA
MseI 689 TTAA T^TAA
NdeI 346 CATATG CA^TATG
Psp1406I 682 AACGTT AA^CGTT
PvuII 799 CAGCTG CAG^CTG
SacI 533 GAGCTC GAGCT^C
SacII 158 CCGCGG CCGC^GG
SapI 434 GCTCTTC GCTCTTC(1/4)
SfcI 527 CTGTAG C^T[GA][CT]AG
StyI 392 CCAAGG C^C[AT][AT]GG
TfiI 867 GATTC G^A[AT]TC
Tru9I 689 TTAA T^TAA
Tsp45I 539 GTCAC ^GT[GC]AC
Tsp509I 692 AATT ^AATT
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# TWO CUT ENZYMES ( 12 enzymes) #
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AvaII 188 GGTCC G^G[AT]CC
211 GGTCC
BfaI 607 CTAG C^TAG
772 CTAG
BseRI 80 CTCCTC GAGGAG(10/8)
515 GAGGAG
BssSI 56 CACGAG CACGAG(-5/-1)
856 CACGAG
EarI 435 CTCTTC CTCTTC(1/4)
708 CTCTTC
Ksp632I 435 CTCTTC CTCTTC(1/4)
708 CTCTTC
MaeI 607 CTAG C^TAG
772 CTAG
MslI 856 CACGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
871 CACGTCTATG
PleI 739 GACTC GAGTC(4/5)
231 GAGTC
SmaI 64 CCCGGG CCC^GGG
116 CCCGGG
XmaCI 64 CCCGGG C^CCGGG
116 CCCGGG
XmaI 64 CCCGGG C^CCGGG
116 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanII BbvI BcnI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I