Restriction map for
pFN21AE6618
(Circular) (Six-base) MAPSORT of: pFN21AE6618.seq Check: 1492 from: 1 to: 5596
pFN21AE6618 5596 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2229 3406 278
Size: 53 83 186 936 693 1177 2468
Fragments arranged by size:
2468 1177 936 693 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 2688 3723 4096 1563
Size: 1125 1035 373 3063
Fragments arranged by size:
3063 1125 1035 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 3659 5043 5311 1632
Size: 1056 971 1384 268 1917
Fragments arranged by size:
1917 1384 1056 971 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2168 2369 1184
Size: 984 201 4411
Fragments arranged by size:
4411 984 201
AlwNI CAG_nnn'CTG
Cuts at: 2648 3339 4911 2648
Size: 691 1572 3333
Fragments arranged by size:
3333 1572 691
ApaLI G'TGCA_C
Cuts at: 2772 3653 4809 2772
Size: 881 1156 3559
Fragments arranged by size:
3559 1156 881
ApoI r'AATT_y
Cuts at: 1493 2696 2905 3131 3283 5392 1493
Size: 1203 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 1203 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2114 2121 2921 1870
Size: 73 126 45 7 800 4545
Fragments arranged by size:
4545 800 126 73 45 7
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2917 4372 618
Size: 325 217 627 345 785 1455 1842
Fragments arranged by size:
1842 1455 785 627 345 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2542 2817 2915 1391
Size: 730 421 275 98 4072
Fragments arranged by size:
4072 730 421 275 98
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BbvCI CC'TCA_GC
Cuts at: 2649 2649
Size: 5596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2436 4207
Size: 107 122 71 631 605 337 427 1771
Cuts at: 4207 136
Size: 1525
Fragments arranged by size:
1771 1525 631 605 427 337 122 107
71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 2811 3013 2811
Size: 202 5394
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2623 2776 3657 4813 1790
Size: 394 439 153 881 1156 2573
Fragments arranged by size:
2573 1156 881 439 394 153
BmgBI CAC'GTC
Cuts at: 1318 2878 1318
Size: 1560 4036
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2645 4256 1239
Size: 1406 1611 2579
Fragments arranged by size:
2579 1611 1406
Bpu10I CC'TnA_GC
Cuts at: 2030 2301 2649 2030
Size: 271 348 4977
Fragments arranged by size:
4977 348 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 2633 5590 2633
Size: 2957 2639
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 3403 3785 4420
Size: 53 83 186 936 693 1177 382 635
Cuts at: 4420 275
Size: 1451
Fragments arranged by size:
1451 1177 936 693 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2076 2535 1186
Size: 890 459 4247
Fragments arranged by size:
4247 890 459
BseYI C'CCAG_C
Cuts at: 4799 5295 4799
Size: 496 5100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2705 2822 1163
Size: 573 402 567 117 3937
Fragments arranged by size:
3937 573 567 402 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2542 2776 3657 3742 4813 2307
Size: 235 234 881 85 1071 3090
Fragments arranged by size:
3090 1071 881 235 234 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2184 2307 2542 2623 2776 2817
Size: 399 331 63 123 235 81 153 41
Cuts at: 2817 2915 3657 3742 4813 1391
Size: 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 331 235 153 123
98 85 81 63 41
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2951 844
Size: 2107 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3483 1394
Size: 429 315 1345 3507
Fragments arranged by size:
3507 1345 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 2061 2861 3656 4668 2061
Size: 800 795 1012 2989
Fragments arranged by size:
2989 1012 800 795
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2163 513
Size: 552 1098 3946
Fragments arranged by size:
3946 1098 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 2717 3112 3906 3926 2283
Size: 434 395 794 20 3953
Fragments arranged by size:
3953 794 434 395 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2695 2900 2695
Size: 205 5391
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 3043 3932
Size: 54 1425 50 275 18 489 724 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 724 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2446 2719 3526 1353
Size: 1093 273 807 3423
Fragments arranged by size:
3423 1093 807 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2258 4183 4557 4703 387
Size: 1064 138 669 1925 374 146 1280
Fragments arranged by size:
1925 1280 1064 669 374 146 138
EcoICRI GAG'CTC
Cuts at: 2540 2540
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2645 2688 3659 4256 5043 5311 1239
Size: 393 1013 43 971 597 787 268 1524
Fragments arranged by size:
1524 1013 971 787 597 393 268 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 2825
Size: 5596
EcoO109I rG'GnC_Cy
Cuts at: 2066 2291 2429 2066
Size: 225 138 5233
Fragments arranged by size:
5233 225 138
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2479 4237 4269 4311
Size: 32 135 32 875 32 1758 32 42
Cuts at: 4311 4343 1373
Size: 32 2626
Fragments arranged by size:
2626 1758 875 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2364 2940 3186 669
Size: 988 281 426 576 246 3079
Fragments arranged by size:
3079 988 576 426 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 10
Size: 54 1425 343 489 3285
Fragments arranged by size:
3285 1425 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2865 2880 3554 3913 4072 518
Size: 648 1699 15 674 359 159 2042
Fragments arranged by size:
2042 1699 674 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2042 2165 2806 3012 3689 4837 5082 1606
Size: 436 123 641 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 436 245 206 123
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 2352 387
Size: 1965 3631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2749 1348
Size: 1401 4195
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2806 2806
Size: 5596
SacI G_AGCT'C
Cuts at: 2542 2542
Size: 5596
SacII CC_GC'GG
Cuts at: 2166 2166
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2446 2446
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2532 2944 4079 4760 4951 826
Size: 219 1487 412 1135 681 191 1471
Fragments arranged by size:
1487 1471 1135 681 412 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2071 2123 2923 1872
Size: 199 52 800 4545
Fragments arranged by size:
4545 800 199 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 2397 3035 513
Size: 552 1332 638 3074
Fragments arranged by size:
3074 1332 638 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2069 2121 2921 1870
Size: 199 52 800 4545
Fragments arranged by size:
4545 800 199 52
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2227 3404 276
Size: 53 83 186 936 693 1177 2468
Fragments arranged by size:
2468 1177 936 693 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI