Restriction map for
pFN21AE6458
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI MamI MfeI MluI MroI
MslI MspI MunI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI Psp1406I
PstI PvuI SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 837 GTCTAC GT^[AC][GT]AC
AccII 32 CGCG CG^CG
AflII 924 CTTAAG C^TTAAG
AflIII 149 ACATGT A^C[GA][CT]GT
AlwNI 74 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 973 CCTGAGG CC^T[ACGT]AGG
ApaI 274 GGGCCC GGGCC^C
Asp700I 438 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 625 GTGCTC G[AT]GC[AT]^C
BalI 1162 TGGCCA TGG^CCA
BanI 385 GGTGCC G^G[CT][GA]CC
BbrPI 898 CACGTG CAC^GTG
BclI 773 TGATCA T^GATCA
BfrI 924 CTTAAG C^TTAAG
BsaAI 898 CACGTG [CT]AC^GT[GA]
BsiHKAI 625 GTGCTC G[AT]GC[AT]^C
BsmI 714 GAATGC GAATGC(1/-1)
BspLU11I 149 ACATGT A^CATGT
BstF5I 141 GGATG GGATG(2/0)
BstUI 32 CGCG CG^CG
BstXI 395 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 973 CCTGAGG CC^T[ACGT]AGG
CpoI 131 CGGTCCG CG^G[AT]CCG
EaeI 1162 TGGCCA [CT]^GGCC[GA]
EarI 659 CTCTTC CTCTTC(1/4)
Eco81I 973 CCTGAGG CC^T[ACGT]AGG
FbaI 773 TGATCA T^GATCA
FokI 141 GGATG GGATG(9/13)
Ksp632I 659 CTCTTC CTCTTC(1/4)
KspI 31 CCGCGG CCGC^GG
MluNI 1162 TGGCCA TGG^CCA
MscI 1162 TGGCCA TGG^CCA
MvnI 32 CGCG CG^CG
NcoI 1127 CCATGG C^CATGG
NdeI 1291 CATATG CA^TATG
PmaCI 898 CACGTG CAC^GTG
PmlI 898 CACGTG CAC^GTG
PshAI 670 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 131 CGGTCCG CG^G[AT]CCG
SacII 31 CCGCGG CCGC^GG
ScaI 708 AGTACT AGT^ACT
SfcI 735 CTACAG C^T[GA][CT]AG
SmlI 924 CTTAAG C^T[CT][GA]AG
StuI 1139 AGGCCT AGG^CCT
XmnI 438 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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BanII 274 GGGCCC G[GA]GC[CT]^C
730 GGGCTC
BbsI 438 GAAGAC GAAGAC(2/6)
667 GAAGAC
BpmI 316 CTCCAG CTGGAG(16/14)
1069 CTGGAG
BpuAI 438 GAAGAC GAAGAC(2/6)
667 GAAGAC
BseRI 100 GAGGAG GAGGAG(10/8)
1024 GAGGAG
Bsp1407I 647 TGTACA T^GTACA
1205 TGTACA
BspHI 935 TCATGA T^CATGA
1034 TCATGA
BsrGI 647 TGTACA T^GTACA
1205 TGTACA
BstYI 605 GGATCT [GA]^GATC[CT]
1110 GGATCT
CfoI 18 GCGC GCG^C
894 GCGC
EcoRI 463 GAATTC G^AATTC
859 GAATTC
FauI 30 CCCGC CCCGC(4/6)
580 GCGGG
HaeII 17 GGCGCT [GA]GCGC^[CT]
893 AGCGCC
HhaI 18 GCGC GCG^C
894 GCGC
HinP1I 18 GCGC G^CGC
894 GCGC
MflI 605 GGATCT [GA]^GATC[CT]
1110 GGATCT
MseI 817 TTAA T^TAA
925 TTAA
NspI 149 ACATGT [GA]CATG^[CT]
653 GCATGT
PpuMI 398 AGGACCT [GA]G^G[AT]CC[CT]
636 GGGACCT
RcaI 935 TCATGA T^CATGA
1034 TCATGA
SfaNI 142 GATGC GCATC(5/9)
919 GATGC
SspBI 647 TGTACA T^GTACA
1205 TGTACA
TaqI 665 TCGA T^CGA
850 TCGA
Tru9I 817 TTAA T^TAA
925 TTAA
TthHB8I 665 TCGA T^CGA
850 TCGA
XbaI 531 TCTAGA T^CTAGA
855 TCTAGA
XcmI 833 CCACGTCTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
930 CCAGCTCATGAATGG
XhoII 605 GGATCT [GA]^GATC[CT]
1110 GGATCT
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI StyI TfiI TseI Tsp45I
Tsp509I TspRI