(Circular) (Six-base) MAPSORT of: pFN21AE6458.seq Check: 8288 from: 1 to: 6061 pFN21AE6458 6061 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2843 3404 1937 Size: 906 561 4594 Fragments arranged by size: 4594 906 561 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2406 2437 2462 3182 3215 3350 4124 Size: 578 196 31 25 720 33 135 774 Cuts at: 4124 5508 5776 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 774 720 578 268 196 135 33 31 25 AflII C'TTAA_G Cuts at: 820 1017 2929 820 Size: 197 1912 3952 Fragments arranged by size: 3952 1912 197 AflIII A'CryG_T Cuts at: 1203 2154 4960 5937 1203 Size: 951 2806 977 1327 Fragments arranged by size: 2806 1327 977 951 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2084 3804 5376 2084 Size: 1720 1572 2769 Fragments arranged by size: 2769 1720 1572 ApaI G_GGCC'C Cuts at: 2283 2283 Size: 6061 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2468 2864 3182 3370 3596 3748 5857 1493 Size: 975 396 318 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 396 318 226 188 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2390 3382 4837 618 Size: 325 217 627 603 992 1455 1842 Fragments arranged by size: 1842 1455 992 627 603 325 217 BanII G_rGCy'C Cuts at: 1391 2283 2739 3380 1391 Size: 892 456 641 4072 Fragments arranged by size: 4072 892 641 456 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2450 2679 928 Size: 971 551 229 4310 Fragments arranged by size: 4310 971 551 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 2778 1548 Size: 1230 4831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2283 2363 4122 5278 1790 Size: 493 80 1759 1156 2573 Fragments arranged by size: 2573 1759 1156 493 80 BmgBI CAC'GTC Cuts at: 1318 2841 1318 Size: 1523 4538 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2373 3792 4750 Size: 416 53 83 399 24 956 1419 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1753 1419 958 956 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 3095 4721 1239 Size: 1067 789 1626 2579 Fragments arranged by size: 2579 1626 1067 789 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4186 5051 5349 4186 Size: 865 298 4898 Fragments arranged by size: 4898 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2905 3852 493 Size: 711 1701 947 2702 Fragments arranged by size: 2702 1701 947 711 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 3044 1186 Size: 934 924 4203 Fragments arranged by size: 4203 934 924 BseYI C'CCAG_C Cuts at: 2065 5264 5760 2065 Size: 3199 496 2366 Fragments arranged by size: 3199 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2634 4122 4207 5278 2634 Size: 1488 85 1071 3417 Fragments arranged by size: 3417 1488 1071 85 BsmI GAATG_Cn' Cuts at: 2725 3572 3665 2725 Size: 847 93 5121 Fragments arranged by size: 5121 847 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2363 2634 2739 3380 4122 4207 Size: 399 493 80 271 105 641 742 85 Cuts at: 4207 5278 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 641 493 399 271 105 85 80 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 2940 3039 3950 5680 2940 Size: 99 911 1730 3321 Fragments arranged by size: 3321 1730 911 99 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 844 Size: 2572 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4556 4730 65 Size: 1183 3308 174 1396 Fragments arranged by size: 3308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2652 3210 96 Size: 2556 558 2947 Fragments arranged by size: 2947 2556 558 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2407 2011 Size: 396 5665 BstYI r'GATC_y Cuts at: 1972 2610 3115 3391 3758 4143 4160 5612 5698 Size: 638 505 276 367 385 17 1452 86 Cuts at: 5698 5710 6056 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 638 505 385 367 346 276 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2979 2979 Size: 6061 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 2303 2355 3132 513 Size: 552 939 32 267 52 777 3442 Fragments arranged by size: 3442 939 777 552 267 52 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 2015 3577 4371 4391 2015 Size: 1562 794 20 3685 Fragments arranged by size: 3685 1562 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2867 2902 529 Size: 35 2303 35 3688 Fragments arranged by size: 3688 2303 35 35 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3167 3508 4397 Size: 54 1425 50 275 18 1337 341 889 Cuts at: 4397 6026 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1337 889 341 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2670 3991 1353 Size: 1317 1321 3423 Fragments arranged by size: 3423 1321 1317 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2306 2406 2437 2462 3095 3182 Size: 393 578 96 100 31 25 633 87 Cuts at: 3182 3215 3350 4124 4721 5508 5776 1239 Size: 33 135 774 597 787 268 1524 Fragments arranged by size: 1524 787 774 633 597 578 393 268 135 100 96 87 33 31 25 EcoO109I rG'GnC_Cy Cuts at: 2280 2404 2642 3205 2280 Size: 124 238 563 5136 Fragments arranged by size: 5136 563 238 124 EcoRI G'AATT_C Cuts at: 2468 2864 3370 3748 2468 Size: 396 506 378 4781 Fragments arranged by size: 4781 506 396 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2596 2467 Size: 129 5932 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2026 2902 5208 1678 Size: 348 876 2306 2531 Fragments arranged by size: 2531 2306 876 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2553 2585 2972 3004 3209 Size: 32 135 32 981 32 387 32 205 Cuts at: 3209 3241 4702 4734 4776 4808 1373 Size: 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 981 387 205 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3169 10 Size: 54 1425 343 1337 2902 Fragments arranged by size: 2902 1425 1337 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4019 4378 4537 518 Size: 648 2853 359 159 2042 Fragments arranged by size: 2853 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2059 2525 3112 3193 3477 4154 5302 Size: 432 21 466 587 81 284 677 1148 Cuts at: 5302 5547 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 587 466 432 284 245 81 21 NcoI C'CATG_G Cuts at: 513 1065 2004 3132 513 Size: 552 939 1128 3442 Fragments arranged by size: 3442 1128 939 552 NdeI CA'TA_TG Cuts at: 387 3297 387 Size: 2910 3151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2158 2662 3419 4964 1132 Size: 97 929 504 757 1545 2229 Fragments arranged by size: 2229 1545 929 757 504 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 2154 4960 2154 Size: 2806 3255 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6061 PfoI T'CCnGG_A Cuts at: 2606 2606 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PmlI CAC'GTG Cuts at: 2905 2905 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 2642 2404 Size: 238 5823 PshAI GACnn'nnGTC Cuts at: 1613 2679 1613 Size: 1066 4995 PsiI TTA'TAA Cuts at: 2547 3631 2547 Size: 1084 4977 PspOMI G'GGCC_C Cuts at: 2279 2279 Size: 6061 PstI C_TGCA'G Cuts at: 830 3413 830 Size: 2583 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2525 3112 3193 2525 Size: 587 81 5393 Fragments arranged by size: 5393 587 81 RsrII CG'GwC_CG Cuts at: 2137 2137 Size: 6061 SacII CC_GC'GG Cuts at: 2039 2039 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 2715 4309 6021 2715 Size: 1594 1712 2755 Fragments arranged by size: 2755 1712 1594 SfcI C'TryA_G Cuts at: 826 1045 2740 3409 4544 5225 5416 826 Size: 219 1695 669 1135 681 191 1471 Fragments arranged by size: 1695 1471 1135 681 669 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2929 4165 5066 5328 820 Size: 197 926 986 1236 901 262 1553 Fragments arranged by size: 1553 1236 986 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 3146 1517 Size: 1629 4432 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2595 3132 3313 3500 513 Size: 552 939 396 195 537 181 187 3074 Fragments arranged by size: 3074 939 552 537 396 195 187 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2588 3139 4088 4105 4258 4443 4888 Size: 1255 1008 551 949 17 153 185 445 Cuts at: 4888 325 Size: 1498 Fragments arranged by size: 1498 1255 1008 949 551 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2652 2713 3210 Size: 274 80 33 51 1443 675 61 497 Cuts at: 3210 4307 6019 96 Size: 1097 1712 138 Fragments arranged by size: 1712 1443 1097 675 497 274 138 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 2536 2860 3397 2536 Size: 324 537 5200 Fragments arranged by size: 5200 537 324 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2845 2942 2845 Size: 97 5964 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2447 4190 2447 Size: 1743 4318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI