Restriction map for
pFN21AE6458
(Linear) (Six-base) MAPSORT of: KIEE6458.seq Check: 4323 from: 1 to: 1356
KIEE6458.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AccI GT'mk_AC
Cuts at: 0 838 1356
Size: 838 518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 401 432 457 1177 1210 1345 1356
Size: 205 196 31 25 720 33 135 11
Fragments arranged by size:
720 205 196 135 33 31 25 11
AflII C'TTAA_G
Cuts at: 0 924 1356
Size: 924 432
AflIII A'CryG_T
Cuts at: 0 149 1356
Size: 149 1207
AlwNI CAG_nnn'CTG
Cuts at: 0 79 1356
Size: 79 1277
ApaI G_GGCC'C
Cuts at: 0 278 1356
Size: 278 1078
ApoI r'AATT_y
Cuts at: 0 463 859 1177 1356
Size: 463 396 318 179
Fragments arranged by size:
463 396 318 179
BanI G'GyrC_C
Cuts at: 0 385 1356
Size: 385 971
BanII G_rGCy'C
Cuts at: 0 278 734 1356
Size: 278 456 622
Fragments arranged by size:
622 456 278
BbsI GAAGACnn'nnnn_
Cuts at: 0 445 674 1356
Size: 445 229 682
Fragments arranged by size:
682 445 229
BclI T'GATC_A
Cuts at: 0 773 1356
Size: 773 583
Bme1580I G_kGCm'C
Cuts at: 0 278 358 1356
Size: 278 80 998
Fragments arranged by size:
998 278 80
BmgBI CAC'GTC
Cuts at: 0 836 1356
Size: 836 520
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 1356
Size: 368 988
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1090 1356
Size: 301 789 266
Fragments arranged by size:
789 301 266
BsaAI yAC'GTr
Cuts at: 0 900 1356
Size: 900 456
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 1039 1356
Size: 115 924 317
Fragments arranged by size:
924 317 115
BseYI C'CCAG_C
Cuts at: 0 60 1356
Size: 60 1296
BsiHKAI G_wGCw'C
Cuts at: 0 629 1356
Size: 629 727
BsmI GAATG_Cn'
Cuts at: 0 720 1356
Size: 720 636
Bsp1286I G_dGCh'C
Cuts at: 0 278 358 629 734 1356
Size: 278 80 271 105 622
Fragments arranged by size:
622 278 271 105 80
BspHI T'CATG_A
Cuts at: 0 935 1034 1356
Size: 935 99 322
Fragments arranged by size:
935 322 99
BsrGI T'GTAC_A
Cuts at: 0 647 1205 1356
Size: 647 558 151
Fragments arranged by size:
647 558 151
BstXI CCAn_nnnn'nTGG
Cuts at: 0 402 1356
Size: 402 954
BstYI r'GATC_y
Cuts at: 0 605 1110 1356
Size: 605 505 246
Fragments arranged by size:
605 505 246
Bsu36I CC'TnA_GG
Cuts at: 0 974 1356
Size: 974 382
BtgI C'CryG_G
Cuts at: 0 31 298 350 1127 1356
Size: 31 267 52 777 229
Fragments arranged by size:
777 267 229 52 31
BtsI GCAGTG_nn'
Cuts at: 0 10 1356
Size: 10 1346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 862 897 1356
Size: 862 35 459
Fragments arranged by size:
862 459 35
EaeI y'GGCC_r
Cuts at: 0 1162 1356
Size: 1162 194
EarI CTCTTCn'nnn_
Cuts at: 0 665 1356
Size: 665 691
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 301 401 432 457 1090 1177 1210
Size: 205 96 100 31 25 633 87 33
Cuts at: 1210 1345 1356
Size: 135 11
Fragments arranged by size:
633 205 135 100 96 87 33 31
25 11
EcoO109I rG'GnC_Cy
Cuts at: 0 275 399 637 1200 1356
Size: 275 124 238 563 156
Fragments arranged by size:
563 275 238 156 124
EcoRI G'AATT_C
Cuts at: 0 463 859 1356
Size: 463 396 497
Fragments arranged by size:
497 463 396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 591 1356
Size: 462 129 765
Fragments arranged by size:
765 462 129
HaeII r_GCGC'y
Cuts at: 0 21 897 1356
Size: 21 876 459
Fragments arranged by size:
876 459 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 548 580 967 999 1204 1236 1356
Size: 548 32 387 32 205 32 120
Fragments arranged by size:
548 387 205 120 32 32 32
MscI TGG'CCA
Cuts at: 0 1164 1356
Size: 1164 192
MspA1I CmG'CkG
Cuts at: 0 33 54 520 1107 1188 1356
Size: 33 21 466 587 81 168
Fragments arranged by size:
587 466 168 81 33 21
NcoI C'CATG_G
Cuts at: 0 1127 1356
Size: 1127 229
NdeI CA'TA_TG
Cuts at: 0 1292 1356
Size: 1292 64
NspI r_CATG'y
Cuts at: 0 153 657 1356
Size: 153 504 699
Fragments arranged by size:
699 504 153
PciI A'CATG_T
Cuts at: 0 149 1356
Size: 149 1207
PfoI T'CCnGG_A
Cuts at: 0 601 1356
Size: 601 755
PmlI CAC'GTG
Cuts at: 0 900 1356
Size: 900 456
PpuMI rG'GwC_Cy
Cuts at: 0 399 637 1356
Size: 399 238 719
Fragments arranged by size:
719 399 238
PshAI GACnn'nnGTC
Cuts at: 0 674 1356
Size: 674 682
PsiI TTA'TAA
Cuts at: 0 542 1356
Size: 542 814
PspOMI G'GGCC_C
Cuts at: 0 274 1356
Size: 274 1082
PvuII CAG'CTG
Cuts at: 0 520 1107 1188 1356
Size: 520 587 81 168
Fragments arranged by size:
587 520 168 81
RsrII CG'GwC_CG
Cuts at: 0 132 1356
Size: 132 1224
SacII CC_GC'GG
Cuts at: 0 34 1356
Size: 34 1322
ScaI AGT'ACT
Cuts at: 0 710 1356
Size: 710 646
SfcI C'TryA_G
Cuts at: 0 735 1356
Size: 735 621
SmlI C'TyrA_G
Cuts at: 0 924 1356
Size: 924 432
StuI AGG'CCT
Cuts at: 0 1141 1356
Size: 1141 215
StyI C'CwwG_G
Cuts at: 0 395 590 1127 1308 1356
Size: 395 195 537 181 48
Fragments arranged by size:
537 395 195 181 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 583 1134 1356
Size: 583 551 222
Fragments arranged by size:
583 551 222
TatI w'GTAC_w
Cuts at: 0 647 708 1205 1356
Size: 647 61 497 151
Fragments arranged by size:
647 497 151 61
XbaI T'CTAG_A
Cuts at: 0 531 855 1356
Size: 531 324 501
Fragments arranged by size:
531 501 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 937 1356
Size: 840 97 419
Fragments arranged by size:
840 419 97
XmnI GAAnn'nnTTC
Cuts at: 0 442 1356
Size: 442 914
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaI ApoI BanI
BanII BbsI BclI Bme1580I BmgBI BmrI BpmI BsaAI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrGI BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EarI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I MscI MspA1I NcoI
NdeI NspI PciI PfoI PmlI PpuMI PshAI PsiI
PspOMI PvuII RsrII SacII ScaI SfcI SmlI StuI
StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PpiI PspXI PsrI PstI PvuI
SacI SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XhoI XmaI ZraI