Restriction map for
pFN21AE3244
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I ApaLI
AscI Asp700I Asp718I AspEI AspI
AvrII BbrPI BbsI BbvCI BcgI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrGI BssSI Bst1107I BstEII BstPI
BstZ17I CelII CpoI DraIII DsaI
Eam1105I EcoO65I EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NotI NruI NsiI PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflII 1083 CTTAAG C^TTAAG
AlwNI 405 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 294 CCTCAGG CC^T[ACGT]AGG
Aor51HI 311 AGCGCT AGC^GCT
AvaI 815 CTCGAG C^[CT]CG[GA]G
AviII 996 TGCGCA TGC^GCA
BalI 668 TGGCCA TGG^CCA
BamHI 380 GGATCC G^GATCC
BfrI 1083 CTTAAG C^TTAAG
BseRI 764 CTCCTC GAGGAG(10/8)
BsmAI 1393 GAGAC GTCTC(1/5)
BsoBI 815 CTCGAG C^[CT]CG[GA]G
BspDI 1043 ATCGAT AT^CGAT
BsrDI 1618 GCAATG GCAATG(2/0)
BssHII 230 GCGCGC G^CGCGC
BstBI 1496 TTCGAA TT^CGAA
Bsu36I 294 CCTCAGG CC^T[ACGT]AGG
ClaI 1043 ATCGAT AT^CGAT
DraI 513 TTTAAA TTT^AAA
DrdI 346 GACACCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 16 CGGCCG C^GGCCG
EarI 794 CTCTTC CTCTTC(1/4)
EclXI 16 CGGCCG C^GGCCG
Eco47III 311 AGCGCT AGC^GCT
Eco52I 16 CGGCCG C^GGCCG
Eco81I 294 CCTCAGG CC^T[ACGT]AGG
EcoNI 294 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 269 GCGGG CCCGC(4/6)
FspI 996 TGCGCA TGC^GCA
HincII 440 GTCAAC GT[CT]^[GA]AC
HindII 440 GTCAAC GT[CT]^[GA]AC
Ksp632I 794 CTCTTC CTCTTC(1/4)
MaeII 392 ACGT A^CGT
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MslI 1414 CATGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 624 GCTAGC G^CTAGC
NspI 1614 ACATGC [GA]CATG^[CT]
NspV 1496 TTCGAA TT^CGAA
PaeR7I 815 CTCGAG C^TCGAG
PshAI 1417 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 142 CAGCTG CAG^CTG
SfuI 1496 TTCGAA TT^CGAA
Tsp45I 1594 GTCAC ^GT[GC]AC
XhoI 815 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 1574 GGGCCC GGGCC^C
1582 GGGCCC
AseI 583 ATTAAT AT^TAAT
657 ATTAAT
AsnI 583 ATTAAT AT^TAAT
657 ATTAAT
BbeI 21 GGCGCC GGCGC^C
250 GGCGCC
BciVI 1072 GGATAC GTATCC(6/5)
1348 GGATAC
BcnI 25 CCGGG CC^[GC]GG
384 CCGGG
BsiEI 16 CGGCCG CG[GA][CT]^CG
130 CGGTCG
BspMI 999 GCAGGT ACCTGC(4/8)
1554 GCAGGT
BstXI 350 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
950 CCAATACATTGG
BstYI 380 GGATCC [GA]^GATC[CT]
722 GGATCT
EaeI 16 CGGCCG [CT]^GGCC[GA]
668 TGGCCA
HgaI 75 GACGC GACGC(5/10)
361 GACGC
HindIII 448 AAGCTT A^AGCTT
634 AAGCTT
HinfI 732 GAATC G^A[ACGT]TC
1470 GATTC
KasI 21 GGCGCC G^GCGCC
250 GGCGCC
MflI 380 GGATCC [GA]^GATC[CT]
722 GGATCT
MspA1I 127 CAGCGG C[AC]G^C[GT]G
142 CAGCTG
NaeI 18 GCCGGC GCC^GGC
137 GCCGGC
NarI 21 GGCGCC GG^CGCC
250 GGCGCC
NciI 25 CCGGG CC^[GC]GG
384 CCGGG
NgoMIV 18 GCCGGC G^CCGGC
137 GCCGGC
PpuMI 353 AGGTCCT [GA]G^G[AT]CC[CT]
854 GGGTCCT
PshBI 583 ATTAAT AT^TAAT
657 ATTAAT
PstI 244 CTGCAG CTGCA^G
1365 CTGCAG
ScaI 819 AGTACT AGT^ACT
1666 AGTACT
SfoI 21 GGCGCC GGC^GCC
250 GGCGCC
TfiI 732 GAATC G^A[AT]TC
1470 GATTC
XhoII 380 GGATCC [GA]^GATC[CT]
722 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I