(Circular) (Six-base) MAPSORT of: pFN21AE3244.seq Check: 7161 from: 1 to: 6406 pFN21AE3244 6406 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3550 3550 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3723 4469 5853 6121 1632 Size: 2091 746 1384 268 1917 Fragments arranged by size: 2091 1917 1384 746 268 AfeI AGC'GCT Cuts at: 2318 2318 Size: 6406 AflII C'TTAA_G Cuts at: 820 1017 3088 820 Size: 197 2071 4138 Fragments arranged by size: 4138 2071 197 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AlwNI CAG_nnn'CTG Cuts at: 2415 4149 5721 2415 Size: 1734 1572 3100 Fragments arranged by size: 3100 1734 1572 ApaI G_GGCC'C Cuts at: 3583 3591 3583 Size: 8 6398 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2546 2720 2991 3104 3204 3715 3941 4093 Size: 1053 174 271 113 100 511 226 152 Cuts at: 4093 6202 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1053 511 271 226 174 152 113 100 AseI AT'TA_AT Cuts at: 160 1032 2589 2663 4960 160 Size: 872 1557 74 2297 1606 Fragments arranged by size: 2297 1606 1557 872 74 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2820 3731 1870 Size: 73 877 911 4545 Fragments arranged by size: 4545 911 877 73 BamHI G'GATC_C Cuts at: 2385 3736 4103 2385 Size: 1351 367 4688 Fragments arranged by size: 4688 1351 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2064 2255 2478 3181 Size: 325 217 627 239 38 191 223 703 Cuts at: 3181 3727 5182 618 Size: 546 1455 1842 Fragments arranged by size: 1842 1455 703 627 546 325 239 223 217 191 38 BanII G_rGCy'C Cuts at: 1391 2036 2280 3583 3591 3725 1391 Size: 645 244 1303 8 134 4072 Fragments arranged by size: 4072 1303 645 244 134 8 BbeI G_GCGC'C Cuts at: 2030 2259 2030 Size: 229 6177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5435 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3071 3347 4298 5508 3071 Size: 276 951 1210 3969 Fragments arranged by size: 3969 1210 951 276 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2067 2403 3583 3591 4467 5623 1790 Size: 277 336 1180 8 876 1156 2573 Fragments arranged by size: 2573 1180 1156 876 336 277 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3367 4137 5095 Size: 416 53 83 399 24 1950 770 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1950 1753 958 770 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2633 1056 Size: 1577 4829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 3006 3663 5066 1239 Size: 875 892 657 1403 2579 Fragments arranged by size: 2579 1403 892 875 657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 4531 5396 5694 2327 Size: 2204 865 298 3039 Fragments arranged by size: 3039 2204 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5069 882 Size: 617 3570 2219 Fragments arranged by size: 3570 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2256 2367 4213 Size: 53 83 186 936 494 229 111 1846 Cuts at: 4213 4595 5230 275 Size: 382 635 1451 Fragments arranged by size: 1846 1451 936 635 494 382 229 186 111 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2752 2782 1178 Size: 30 165 30 1349 30 4802 Fragments arranged by size: 4802 1349 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2760 1186 Size: 1574 4832 BseYI C'CCAG_C Cuts at: 3320 5609 6105 3320 Size: 2289 496 3621 Fragments arranged by size: 3621 2289 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2428 3577 1163 Size: 573 399 293 1149 3992 Fragments arranged by size: 3992 1149 573 399 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2024 2138 3856 4617 5645 1540 Size: 275 186 23 114 1718 761 1028 2301 Fragments arranged by size: 2301 1718 1028 761 275 186 114 23 BsiHKAI G_wGCw'C Cuts at: 2090 3472 3545 4467 4552 5623 2090 Size: 1382 73 922 85 1071 2873 Fragments arranged by size: 2873 1382 1071 922 85 73 BsmI GAATG_Cn' Cuts at: 3917 4010 3917 Size: 93 6313 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2067 2090 2280 2403 3472 3545 Size: 399 246 31 23 190 123 1069 73 Cuts at: 3545 3583 3591 3725 4467 4552 5623 1391 Size: 38 8 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 1069 742 399 246 190 134 123 85 73 38 31 23 8 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2995 3550 3761 844 Size: 2151 555 211 3489 Fragments arranged by size: 3489 2151 555 211 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3630 4901 5075 65 Size: 1183 2382 1271 174 1396 Fragments arranged by size: 2382 1396 1271 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 2101 2142 5050 5237 1700 Size: 110 213 78 41 2908 187 2869 Fragments arranged by size: 2908 2869 213 187 110 78 41 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 2235 1925 Size: 310 6096 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2248 2478 3010 794 Size: 1454 230 532 4190 Fragments arranged by size: 4190 1454 532 230 BstBI TT'CG_AA Cuts at: 3502 5210 3502 Size: 1708 4698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstXI CCAn_nnnn'nTGG Cuts at: 2362 2962 2362 Size: 600 5806 BstYI r'GATC_y Cuts at: 1972 2385 2727 3736 4103 4488 4505 5957 6043 Size: 413 342 1009 367 385 17 1452 86 Cuts at: 6043 6055 6401 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1009 413 385 367 346 342 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2300 2300 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4915 Fragments arranged by size: 4915 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 2441 2657 3314 3922 4716 4736 2441 Size: 216 657 608 794 20 4111 Fragments arranged by size: 4111 794 657 608 216 20 ClaI AT'CG_AT Cuts at: 1554 3049 1554 Size: 1495 4911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3543 3578 529 Size: 35 2979 35 3357 Fragments arranged by size: 3357 2979 35 35 DraI TTT'AAA Cuts at: 2520 3710 2520 Size: 1190 5216 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2357 5413 809 Size: 507 1041 3056 1802 Fragments arranged by size: 3056 1802 1041 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2021 2673 3853 Size: 54 1425 50 275 18 191 652 1180 Cuts at: 3853 4742 6371 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1180 889 652 275 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2021 3853 1537 Size: 275 209 1832 4090 Fragments arranged by size: 4090 1832 275 209 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 4336 1353 Size: 1452 1531 3423 Fragments arranged by size: 3423 1531 1452 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 3006 3663 3723 4469 5066 5853 Size: 393 482 892 657 60 746 597 787 Cuts at: 5853 6121 1239 Size: 268 1524 Fragments arranged by size: 1524 892 787 746 657 597 482 393 268 60 EcoNI CCTnn'n_nnAGG Cuts at: 2303 2303 Size: 6406 EcoO109I rG'GnC_Cy Cuts at: 2047 2359 2860 3587 3588 2047 Size: 312 501 727 1 4865 Fragments arranged by size: 4865 727 501 312 1 EcoRI G'AATT_C Cuts at: 2546 3104 3204 3715 4093 2546 Size: 558 100 511 378 4859 Fragments arranged by size: 4859 558 511 378 100 FspI TGC'GCA Cuts at: 3003 4912 3003 Size: 1909 4497 HaeII r_GCGC'y Cuts at: 1678 2030 2259 2320 5553 1678 Size: 352 229 61 3233 2531 Fragments arranged by size: 3233 2531 352 229 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3391 3423 3650 3682 5047 Size: 32 135 32 1819 32 227 32 1365 Cuts at: 5047 5079 5121 5153 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1819 1365 227 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2447 3750 3996 669 Size: 988 281 509 1303 246 3079 Fragments arranged by size: 3079 1303 988 509 281 246 HindIII A'AGCT_T Cuts at: 748 2453 2639 748 Size: 1705 186 4515 Fragments arranged by size: 4515 1705 186 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KasI G'GCGC_C Cuts at: 2026 2255 2026 Size: 229 6177 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3112 3424 5520 5704 1305 Size: 1807 312 2096 184 2007 Fragments arranged by size: 2096 2007 1807 312 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 3741 Fragments arranged by size: 3741 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3423 4364 4723 4882 518 Size: 648 2257 941 359 159 2042 Fragments arranged by size: 2257 2042 941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2149 3822 4499 5647 5892 1606 Size: 528 15 1673 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 677 528 245 15 NaeI GCC'GGC Cuts at: 2025 2144 2025 Size: 119 6287 NarI GG'CG_CC Cuts at: 2027 2256 2027 Size: 229 6177 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4915 Fragments arranged by size: 4915 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NgoMIV G'CCGG_C Cuts at: 2023 2142 2023 Size: 119 6287 NheI G'CTAG_C Cuts at: 1052 2629 1052 Size: 1577 4829 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3623 3764 5309 1132 Size: 97 2394 141 1545 2229 Fragments arranged by size: 2394 2229 1545 141 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6406 PfoI T'CCnGG_A Cuts at: 2361 2862 2361 Size: 501 5905 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2860 2359 Size: 501 5905 PshAI GACnn'nnGTC Cuts at: 1613 3426 1613 Size: 1813 4593 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PspOMI G'GGCC_C Cuts at: 3579 3587 3579 Size: 8 6398 PspXI vC'TCGA_Gb Cuts at: 2820 2820 Size: 6406 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3338 3370 3338 Size: 32 6374 PstI C_TGCA'G Cuts at: 830 2253 3374 3758 830 Size: 1423 1121 384 3478 Fragments arranged by size: 3478 1423 1121 384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2149 2149 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 2826 3673 4654 6366 2826 Size: 847 981 1712 2866 Fragments arranged by size: 2866 1712 981 847 SfcI C'TryA_G Cuts at: 826 1045 2249 2850 3370 3754 4889 5570 5761 Size: 219 1204 601 520 384 1135 681 191 Cuts at: 5761 826 Size: 1471 Fragments arranged by size: 1471 1204 1135 681 601 520 384 219 191 SfoI GGC'GCC Cuts at: 2028 2257 2028 Size: 229 6177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 2820 3088 4510 5411 5673 Size: 197 926 399 478 268 1422 901 262 Cuts at: 5673 820 Size: 1553 Fragments arranged by size: 1553 1422 926 901 478 399 268 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6406 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 3099 3575 3583 3845 513 Size: 552 939 351 744 476 8 262 3074 Fragments arranged by size: 3074 939 744 552 476 351 262 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 4433 4450 4603 4788 5233 325 Size: 1255 500 2353 17 153 185 445 1498 Fragments arranged by size: 2353 1498 1255 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2491 2795 2824 Size: 274 80 33 51 1443 514 304 29 Cuts at: 2824 2904 3671 4652 6364 96 Size: 80 767 981 1712 138 Fragments arranged by size: 1712 1443 981 767 514 304 274 138 80 80 51 33 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6366 6398 6366 Size: 32 6374 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 2820 1943 Size: 877 5529 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI