(Linear) (Six-base) MAPSORT of: KIEE3244.seq Check: 9782 from: 1 to: 1701 KIEE3244.orf With 182 enzymes: SgfI * July 3, 2012 17:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1545 1701 Size: 1545 156 AfeI AGC'GCT Cuts at: 0 313 1701 Size: 313 1388 AflII C'TTAA_G Cuts at: 0 1083 1701 Size: 1083 618 AlwNI CAG_nnn'CTG Cuts at: 0 410 1701 Size: 410 1291 ApaI G_GGCC'C Cuts at: 0 1578 1586 1701 Size: 1578 8 115 Fragments arranged by size: 1578 115 8 ApoI r'AATT_y Cuts at: 0 541 715 986 1099 1199 1701 Size: 541 174 271 113 100 502 Fragments arranged by size: 541 502 271 174 113 100 AseI AT'TA_AT Cuts at: 0 584 658 1701 Size: 584 74 1043 Fragments arranged by size: 1043 584 74 AvaI C'yCGr_G Cuts at: 0 815 1701 Size: 815 886 BamHI G'GATC_C Cuts at: 0 380 1701 Size: 380 1321 BanI G'GyrC_C Cuts at: 0 21 59 250 473 1176 1701 Size: 21 38 191 223 703 525 Fragments arranged by size: 703 525 223 191 38 21 BanII G_rGCy'C Cuts at: 0 31 275 1578 1586 1701 Size: 31 244 1303 8 115 Fragments arranged by size: 1303 244 115 31 8 BbeI G_GCGC'C Cuts at: 0 25 254 1701 Size: 25 229 1447 Fragments arranged by size: 1447 229 25 BciVI GTATCCnnnnn_n' Cuts at: 0 1066 1342 1701 Size: 1066 276 359 Fragments arranged by size: 1066 359 276 Bme1580I G_kGCm'C Cuts at: 0 62 398 1578 1586 1701 Size: 62 336 1180 8 115 Fragments arranged by size: 1180 336 115 62 8 BmrI ACTGGGnnnn_n' Cuts at: 0 1362 1701 Size: 1362 339 BmtI G_CTAG'C Cuts at: 0 628 1701 Size: 628 1073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 1001 1658 1701 Size: 109 892 657 43 Fragments arranged by size: 892 657 109 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1701 Size: 322 1379 BsaHI Gr'CG_yC Cuts at: 0 22 251 362 1701 Size: 22 229 111 1339 Fragments arranged by size: 1339 229 111 22 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 747 777 1701 Size: 747 30 924 Fragments arranged by size: 924 747 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 755 1701 Size: 755 946 BseYI C'CCAG_C Cuts at: 0 1315 1701 Size: 1315 386 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 423 1572 1701 Size: 130 293 1149 129 Fragments arranged by size: 1149 293 130 129 BsiEI CG_ry'CG Cuts at: 0 19 133 1701 Size: 19 114 1568 Fragments arranged by size: 1568 114 19 BsiHKAI G_wGCw'C Cuts at: 0 85 1467 1540 1701 Size: 85 1382 73 161 Fragments arranged by size: 1382 161 85 73 Bsp1286I G_dGCh'C Cuts at: 0 31 62 85 275 398 1467 1540 1578 Size: 31 31 23 190 123 1069 73 38 Cuts at: 1578 1586 1701 Size: 8 115 Fragments arranged by size: 1069 190 123 115 73 38 31 31 23 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 990 1545 1701 Size: 990 555 156 Fragments arranged by size: 990 555 156 BsrDI GCAATG_nn' Cuts at: 0 1625 1701 Size: 1625 76 BsrFI r'CCGG_y Cuts at: 0 18 96 137 1701 Size: 18 78 41 1564 Fragments arranged by size: 1564 78 41 18 BssHII G'CGCG_C Cuts at: 0 230 1701 Size: 230 1471 BstAPI GCAn_nnn'nTGC Cuts at: 0 243 473 1005 1701 Size: 243 230 532 696 Fragments arranged by size: 696 532 243 230 BstBI TT'CG_AA Cuts at: 0 1497 1701 Size: 1497 204 BstXI CCAn_nnnn'nTGG Cuts at: 0 357 957 1701 Size: 357 600 744 Fragments arranged by size: 744 600 357 BstYI r'GATC_y Cuts at: 0 380 722 1701 Size: 380 342 979 Fragments arranged by size: 979 380 342 Bsu36I CC'TnA_GG Cuts at: 0 295 1701 Size: 295 1406 BtsI GCAGTG_nn' Cuts at: 0 436 652 1309 1701 Size: 436 216 657 392 Fragments arranged by size: 657 436 392 216 ClaI AT'CG_AT Cuts at: 0 1044 1701 Size: 1044 657 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1538 1573 1701 Size: 1538 35 128 Fragments arranged by size: 1538 128 35 DraI TTT'AAA Cuts at: 0 515 1701 Size: 515 1186 DrdI GACnn_nn'nnGTC Cuts at: 0 352 1701 Size: 352 1349 EaeI y'GGCC_r Cuts at: 0 16 668 1701 Size: 16 652 1033 Fragments arranged by size: 1033 652 16 EagI C'GGCC_G Cuts at: 0 16 1701 Size: 16 1685 EarI CTCTTCn'nnn_ Cuts at: 0 800 1701 Size: 800 901 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 1001 1658 1701 Size: 109 892 657 43 Fragments arranged by size: 892 657 109 43 EcoNI CCTnn'n_nnAGG Cuts at: 0 298 1701 Size: 298 1403 EcoO109I rG'GnC_Cy Cuts at: 0 42 354 855 1582 1583 1701 Size: 42 312 501 727 1 118 Fragments arranged by size: 727 501 312 118 42 1 EcoRI G'AATT_C Cuts at: 0 541 1099 1199 1701 Size: 541 558 100 502 Fragments arranged by size: 558 541 502 100 FspI TGC'GCA Cuts at: 0 998 1701 Size: 998 703 HaeII r_GCGC'y Cuts at: 0 25 254 315 1701 Size: 25 229 61 1386 Fragments arranged by size: 1386 229 61 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1386 1418 1645 1677 1701 Size: 1386 32 227 32 24 Fragments arranged by size: 1386 227 32 32 24 HincII GTy'rAC Cuts at: 0 442 1701 Size: 442 1259 HindIII A'AGCT_T Cuts at: 0 448 634 1701 Size: 448 186 1067 Fragments arranged by size: 1067 448 186 KasI G'GCGC_C Cuts at: 0 21 250 1701 Size: 21 229 1451 Fragments arranged by size: 1451 229 21 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1107 1419 1701 Size: 1107 312 282 Fragments arranged by size: 1107 312 282 MscI TGG'CCA Cuts at: 0 670 1701 Size: 670 1031 MslI CAynn'nnrTG Cuts at: 0 1418 1701 Size: 1418 283 MspA1I CmG'CkG Cuts at: 0 129 144 1701 Size: 129 15 1557 Fragments arranged by size: 1557 129 15 NaeI GCC'GGC Cuts at: 0 20 139 1701 Size: 20 119 1562 Fragments arranged by size: 1562 119 20 NarI GG'CG_CC Cuts at: 0 22 251 1701 Size: 22 229 1450 Fragments arranged by size: 1450 229 22 NgoMIV G'CCGG_C Cuts at: 0 18 137 1701 Size: 18 119 1564 Fragments arranged by size: 1564 119 18 NheI G'CTAG_C Cuts at: 0 624 1701 Size: 624 1077 NspI r_CATG'y Cuts at: 0 1618 1701 Size: 1618 83 PfoI T'CCnGG_A Cuts at: 0 356 857 1701 Size: 356 501 844 Fragments arranged by size: 844 501 356 PpuMI rG'GwC_Cy Cuts at: 0 354 855 1701 Size: 354 501 846 Fragments arranged by size: 846 501 354 PshAI GACnn'nnGTC Cuts at: 0 1421 1701 Size: 1421 280 PspOMI G'GGCC_C Cuts at: 0 1574 1582 1701 Size: 1574 8 119 Fragments arranged by size: 1574 119 8 PspXI vC'TCGA_Gb Cuts at: 0 815 1701 Size: 815 886 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1333 1365 1701 Size: 1333 32 336 Fragments arranged by size: 1333 336 32 PstI C_TGCA'G Cuts at: 0 248 1369 1701 Size: 248 1121 332 Fragments arranged by size: 1121 332 248 PvuII CAG'CTG Cuts at: 0 144 1701 Size: 144 1557 ScaI AGT'ACT Cuts at: 0 821 1668 1701 Size: 821 847 33 Fragments arranged by size: 847 821 33 SfcI C'TryA_G Cuts at: 0 244 845 1365 1701 Size: 244 601 520 336 Fragments arranged by size: 601 520 336 244 SfoI GGC'GCC Cuts at: 0 23 252 1701 Size: 23 229 1449 Fragments arranged by size: 1449 229 23 SmlI C'TyrA_G Cuts at: 0 337 815 1083 1701 Size: 337 478 268 618 Fragments arranged by size: 618 478 337 268 StyI C'CwwG_G Cuts at: 0 350 1094 1570 1578 1701 Size: 350 744 476 8 123 Fragments arranged by size: 744 476 350 123 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 75 1701 Size: 75 1626 TatI w'GTAC_w Cuts at: 0 486 790 819 899 1666 1701 Size: 486 304 29 80 767 35 Fragments arranged by size: 767 486 304 80 35 29 XhoI C'TCGA_G Cuts at: 0 815 1701 Size: 815 886 Enzymes that do cut: AarI AfeI AflII AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BciVI Bme1580I BmrI BmtI BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstAPI BstBI BstXI BstYI Bsu36I BtsI ClaI CspCI DraI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PfoI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII ScaI SfcI SfoI SmlI StyI TaqII TatI XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstEII BstZ17I BtgI BtgZI DraIII EciI EcoICRI EcoRV FalI FseI FspAI HpaI KpnI MfeI MluI NcoI NdeI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XmaI XmnI ZraI