Restriction map for
pFN21AE1937
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 33 CGCG CG^CG
AciI 659 CCGC CCGC(-3/-1)
AflIII 230 ACATGT A^C[GA][CT]GT
AspHI 1409 GAGCTC G[AT]GC[AT]^C
AspI 372 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 979 CCTAGG C^CTAGG
BamHI 24 GGATCC G^GATCC
BanII 1409 GAGCTC G[GA]GC[CT]^C
BglII 945 AGATCT A^GATCT
BlnI 979 CCTAGG C^CTAGG
BmyI 1409 GAGCTC G[AGT]GC[ACT]^C
BseRI 949 CTCCTC GAGGAG(10/8)
BsiHKAI 1409 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1409 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 230 ACATGT A^CATGT
BspMI 764 ACCTGC ACCTGC(4/8)
BsrDI 1233 CATTGC GCAATG(2/0)
Bst1107I 424 GTATAC GTA^TAC
BstUI 33 CGCG CG^CG
BstXI 662 CCATATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 424 GTATAC GTA^TAC
Cac8I 1101 GCTAGC GC[ACGT]^[ACGT]GC
DsaI 41 CCGTGG C^C[GA][CT]GG
HindIII 161 AAGCTT A^AGCTT
MvnI 33 CGCG CG^CG
NdeI 1172 CATATG CA^TATG
NheI 1101 GCTAGC G^CTAGC
NspI 230 ACATGT [GA]CATG^[CT]
PflFI 372 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 955 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 715 CTGCAG CTGCA^G
SacI 1409 GAGCTC GAGCT^C
SexAI 434 ACCTGGT A^CC[AT]GGT
Tth111I 372 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 424 GTATAC GT^[AC][GT]AC
1223 GTCTAC
BbsI 700 GAAGAC GAAGAC(2/6)
1079 GTCTTC
BbvI 1237 GCAGC GCAGC(8/12)
1240 GCAGC
BcnI 21 CCGGG CC^[GC]GG
971 CCCGG
BpmI 1306 CTCCAG CTGGAG(16/14)
74 CTGGAG
BpuAI 700 GAAGAC GAAGAC(2/6)
1079 GTCTTC
BsmAI 496 GAGAC GTCTC(1/5)
264 GTCTC
BstYI 24 GGATCC [GA]^GATC[CT]
945 AGATCT
Fnu4HI 1237 GCAGC GC^[ACGT]GC
1240 GCAGC
HapII 21 CCGG C^CGG
972 CCGG
HpaII 21 CCGG C^CGG
972 CCGG
ItaI 1237 GCAGC GC^[ACGT]GC
1240 GCAGC
MflI 24 GGATCC [GA]^GATC[CT]
945 AGATCT
MspI 21 CCGG C^CGG
972 CCGG
NciI 21 CCGGG CC^[GC]GG
971 CCCGG
PleI 15 GAGTC GAGTC(4/5)
1257 GAGTC
SmlI 941 CTCAAG C^T[CT][GA]AG
1161 CTCAAG
TfiI 315 GAATC G^A[AT]TC
353 GAATC
TseI 1237 GCAGC G^C[AT]GC
1240 GCAGC
XhoII 24 GGATCC [GA]^GATC[CT]
945 AGATCT
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
AvaII BfaI BsaJI BsgI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
EcoT14I FokI HaeIII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I