(Circular) (Six-base) MAPSORT of: pFN21AE1937.seq Check: 9774 from: 1 to: 6133 pFN21AE1937 6133 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 3229 3476 1937 Size: 493 799 247 4594 Fragments arranged by size: 4594 799 493 247 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2112 4196 5580 5848 1632 Size: 480 2084 1384 268 1917 Fragments arranged by size: 2084 1917 1384 480 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 2235 5032 6009 1203 Size: 1032 2797 977 1327 Fragments arranged by size: 2797 1327 1032 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 2118 1166 Size: 952 5181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 2272 2483 3265 3876 5448 2272 Size: 211 782 611 1572 2957 Fragments arranged by size: 2957 1572 782 611 211 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2288 2302 2365 3037 3202 3442 3668 3820 Size: 795 14 63 672 165 240 226 152 Cuts at: 3820 5929 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 795 672 240 226 165 152 63 14 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 AvrII C'CTAG_G Cuts at: 2984 2984 Size: 6133 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3119 3152 3119 Size: 33 6100 BamHI G'GATC_C Cuts at: 2029 3463 3830 2029 Size: 1434 367 4332 Fragments arranged by size: 4332 1434 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3454 4909 618 Size: 325 217 627 1667 1455 1842 Fragments arranged by size: 1842 1667 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3418 3452 1391 Size: 2027 34 4072 Fragments arranged by size: 4072 2027 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2712 3077 928 Size: 971 813 365 3984 Fragments arranged by size: 3984 971 813 365 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2950 6128 2950 Size: 3178 2955 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 4194 5350 1790 Size: 2404 1156 2573 Fragments arranged by size: 2573 2404 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2964 3075 3864 Size: 416 53 83 399 24 1547 111 789 Cuts at: 3864 4822 442 Size: 958 1753 Fragments arranged by size: 1753 1547 958 789 416 399 111 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3110 1056 Size: 2054 4079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 3296 4793 1239 Size: 861 1196 1497 2579 Fragments arranged by size: 2579 1497 1196 861 Bpu10I CC'TnA_GC Cuts at: 3352 3352 Size: 6133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2931 3151 4258 5123 5421 2931 Size: 220 1107 865 298 3643 Fragments arranged by size: 3643 1107 865 298 220 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3940 4322 4957 275 Size: 53 83 186 936 2407 382 635 1451 Fragments arranged by size: 2407 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4566 4964 5238 5385 5796 4566 Size: 398 274 147 411 4903 Fragments arranged by size: 4903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 1178 Size: 30 165 30 624 30 5254 Fragments arranged by size: 5254 624 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2945 1186 Size: 1759 4374 BseYI C'CCAG_C Cuts at: 3278 5336 5832 3278 Size: 2058 496 3579 Fragments arranged by size: 3579 2058 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 2529 2603 1163 Size: 573 528 265 74 4693 Fragments arranged by size: 4693 573 528 265 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3418 4194 4279 5350 3418 Size: 776 85 1071 4201 Fragments arranged by size: 4201 1071 776 85 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3418 3452 4194 4279 5350 1391 Size: 399 1628 34 742 85 1071 2174 Fragments arranged by size: 2174 1628 1071 742 399 85 34 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2778 3488 844 Size: 1934 710 3489 Fragments arranged by size: 3489 1934 710 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3237 4628 4802 65 Size: 1183 1989 1391 174 1396 Fragments arranged by size: 1989 1396 1391 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstXI CCAn_nnnn'nTGG Cuts at: 2674 2674 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2029 2950 3463 3830 4215 4232 5684 5770 Size: 57 921 513 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 921 513 385 367 346 86 57 17 12 BstZ17I GTA'TAC Cuts at: 2431 2431 Size: 6133 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 4600 Fragments arranged by size: 4600 939 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3308 487 Size: 683 56 2082 3312 Fragments arranged by size: 3312 2082 683 56 BtsI GCAGTG_nn' Cuts at: 2166 3649 4443 4463 2166 Size: 1483 794 20 3836 Fragments arranged by size: 3836 1483 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3580 4469 6098 Size: 54 1425 50 275 18 1750 889 1629 Cuts at: 6098 8 Size: 43 Fragments arranged by size: 1750 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4063 1353 Size: 2710 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2653 4720 5094 5240 387 Size: 1064 138 1064 2067 374 146 1280 Fragments arranged by size: 2067 1280 1064 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3416 3416 Size: 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2112 3296 4196 4793 5580 5848 Size: 393 468 12 1184 900 597 787 268 Cuts at: 5848 1239 Size: 1524 Fragments arranged by size: 1524 1184 900 787 597 468 393 268 12 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 5280 1678 Size: 3602 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373 Size: 32 135 32 3202 32 42 32 2626 Fragments arranged by size: 3202 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 2166 748 Size: 1418 4715 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2236 2552 5247 5431 1305 Size: 931 316 2695 184 2007 Fragments arranged by size: 2695 2007 931 316 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4311 Fragments arranged by size: 4311 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2118 2240 2672 3177 4091 4450 4609 Size: 648 952 122 432 505 914 359 159 Cuts at: 4609 518 Size: 2042 Fragments arranged by size: 2042 952 914 648 505 432 359 159 122 MspA1I CmG'CkG Cuts at: 1606 3549 4226 5374 5619 1606 Size: 1943 677 1148 245 2120 Fragments arranged by size: 2120 1943 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4642 Fragments arranged by size: 4642 939 552 NdeI CA'TA_TG Cuts at: 387 3178 387 Size: 2791 3342 NheI G'CTAG_C Cuts at: 1052 3106 1052 Size: 2054 4079 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2239 3491 5036 1132 Size: 97 1010 1252 1545 2229 Fragments arranged by size: 2229 1545 1252 1010 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 2235 5032 2235 Size: 2797 3336 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2964 1613 Size: 1351 4782 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 2724 3485 830 Size: 1894 761 3478 Fragments arranged by size: 3478 1894 761 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 SacI G_AGCT'C Cuts at: 3418 3418 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SexAI A'CCwGG_T Cuts at: 2439 2439 Size: 6133 SfcI C'TryA_G Cuts at: 826 1045 2720 3215 3369 3481 4616 5297 5488 Size: 219 1675 495 154 112 1135 681 191 Cuts at: 5488 826 Size: 1471 Fragments arranged by size: 1675 1471 1135 681 495 219 191 154 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2946 3166 4237 5138 5400 820 Size: 197 926 1003 220 1071 901 262 1553 Fragments arranged by size: 1553 1071 1003 926 901 262 220 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2004 2845 2984 3111 3572 513 Size: 552 939 841 139 127 461 3074 Fragments arranged by size: 3074 939 841 552 461 139 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4160 4177 4330 4515 4960 325 Size: 1255 2580 17 153 185 445 1498 Fragments arranged by size: 2580 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4379 6091 96 Size: 274 80 33 51 1443 2402 1712 138 Fragments arranged by size: 2402 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2376 2408 6093 6125 2376 Size: 32 3685 32 2384 Fragments arranged by size: 3685 2384 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2380 1316 Size: 1064 5069 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI