(Linear) (Six-base) MAPSORT of: KIEE1937.seq  Check: 5364  from: 1  to: 1428



KIEE1937.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:51  ..

AccI GT'mk_AC

Cuts at:      0     425    1224    1428 
   Size:        425     799     204

  Fragments arranged by size: 

                 799     425     204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107    1428 
   Size:        107    1321
 
AflIII A'CryG_T

Cuts at:      0     230    1428 
   Size:        230    1198
 
AleI CACnn'nnGTG

Cuts at:      0     113    1428 
   Size:        113    1315
 
AlwNI CAG_nnn'CTG

Cuts at:      0     267     478    1260    1428 
   Size:        267     211     782     168

  Fragments arranged by size: 

                 782     267     211     168
 
ApoI r'AATT_y

Cuts at:      0     283     297     360    1032    1197    1428 
   Size:        283      14      63     672     165     231

  Fragments arranged by size: 

                 672     283     231     165      63      14
 
AvrII C'CTAG_G

Cuts at:      0     979    1428 
   Size:        979     449
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1114    1147    1428 
   Size:       1114      33     281

  Fragments arranged by size: 

                1114     281      33
 
BamHI G'GATC_C

Cuts at:      0      24    1428 
   Size:         24    1404
 
BanII G_rGCy'C

Cuts at:      0    1413    1428 
   Size:       1413      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     707    1072    1428 
   Size:        707     365     356

  Fragments arranged by size: 

                 707     365     356
 
BglII A'GATC_T

Cuts at:      0     945    1428 
   Size:        945     483
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     959    1070    1428 
   Size:        959     111     358

  Fragments arranged by size: 

                 959     358     111
 
BmtI G_CTAG'C

Cuts at:      0    1105    1428 
   Size:       1105     323
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95    1291    1428 
   Size:         95    1196     137

  Fragments arranged by size: 

                1196     137      95
 
Bpu10I CC'TnA_GC

Cuts at:      0    1347    1428 
   Size:       1347      81
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    1146    1428 
   Size:        926     220     282

  Fragments arranged by size: 

                 926     282     220
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      22      52    1428 
   Size:         22      30    1376

  Fragments arranged by size: 

                1376      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     940    1428 
   Size:        940     488
 
BseYI C'CCAG_C

Cuts at:      0    1273    1428 
   Size:       1273     155
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     524     598    1428 
   Size:        259     265      74     830

  Fragments arranged by size: 

                 830     265     259      74
 
BsiHKAI G_wGCw'C

Cuts at:      0    1413    1428 
   Size:       1413      15
 
Bsp1286I G_dGCh'C

Cuts at:      0    1413    1428 
   Size:       1413      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     773    1428 
   Size:        773     655
 
BsrDI GCAATG_nn'

Cuts at:      0    1232    1428 
   Size:       1232     196
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     669    1428 
   Size:        669     759
 
BstYI r'GATC_y

Cuts at:      0      24     945    1428 
   Size:         24     921     483

  Fragments arranged by size: 

                 921     483      24
 
BstZ17I GTA'TAC

Cuts at:      0     426    1428 
   Size:        426    1002
 
BtgI C'CryG_G

Cuts at:      0      41    1428 
   Size:         41    1387
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1303    1428 
   Size:       1303     125
 
BtsI GCAGTG_nn'

Cuts at:      0     161    1428 
   Size:        161    1267
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     648    1428 
   Size:        648     780
 
EcoICRI GAG'CTC

Cuts at:      0    1411    1428 
   Size:       1411      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     107    1291    1428 
   Size:         95      12    1184     137

  Fragments arranged by size: 

                1184     137      95      12
 
HindIII A'AGCT_T

Cuts at:      0     161    1428 
   Size:        161    1267
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     547    1428 
   Size:        231     316     881

  Fragments arranged by size: 

                 881     316     231
 
MslI CAynn'nnrTG

Cuts at:      0     113     235     667    1172    1428 
   Size:        113     122     432     505     256

  Fragments arranged by size: 

                 505     432     256     122     113
 
NdeI CA'TA_TG

Cuts at:      0    1173    1428 
   Size:       1173     255
 
NheI G'CTAG_C

Cuts at:      0    1101    1428 
   Size:       1101     327
 
NspI r_CATG'y

Cuts at:      0     234    1428 
   Size:        234    1194
 
PciI A'CATG_T

Cuts at:      0     230    1428 
   Size:        230    1198
 
PshAI GACnn'nnGTC

Cuts at:      0     959    1428 
   Size:        959     469
 
PstI C_TGCA'G

Cuts at:      0     719    1428 
   Size:        719     709
 
SacI G_AGCT'C

Cuts at:      0    1413    1428 
   Size:       1413      15
 
SexAI A'CCwGG_T

Cuts at:      0     434    1428 
   Size:        434     994
 
SfcI C'TryA_G

Cuts at:      0     715    1210    1364    1428 
   Size:        715     495     154      64

  Fragments arranged by size: 

                 715     495     154      64
 
SmlI C'TyrA_G

Cuts at:      0     941    1161    1428 
   Size:        941     220     267

  Fragments arranged by size: 

                 941     267     220
 
StyI C'CwwG_G

Cuts at:      0     840     979    1106    1428 
   Size:        840     139     127     322

  Fragments arranged by size: 

                 840     322     139     127
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     371     403    1428 
   Size:        371      32    1025

  Fragments arranged by size: 

                1025     371      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     375    1428 
   Size:        375    1053
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApoI    AvrII     BaeI
    BamHI    BanII     BbsI    BglII     BmrI     BmtI     BpmI   Bpu10I
    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     EciI
  EcoICRI  Eco57MI  HindIII     MmeI     MslI     NdeI     NheI     NspI
     PciI    PshAI     PstI     SacI    SexAI     SfcI     SmlI     StyI
     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BplI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI