Restriction map for
pFN21AE3810
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BpuAI BsaBI
BseRI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1213 CGCG CG^CG
AccIII 356 TCCGGA T^CCGGA
AocI 156 CCTCAGG CC^T[ACGT]AGG
AspI 430 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 307 CCCGAG C^[CT]CG[GA]G
BalI 182 TGGCCA TGG^CCA
BbvCI 477 CCTCAGC CCTCAGC(-5/-2)
BcnI 953 CCGGG CC^[GC]GG
BglI 766 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 846 AGATCT A^GATCT
BpmI 664 CTGGAG CTGGAG(16/14)
BsaAI 1206 CACGTA [CT]AC^GT[GA]
BsaI 1025 GAGACC GGTCTC(1/5)
BsaWI 356 TCCGGA [AT]^CCGG[AT]
BseAI 356 TCCGGA T^CCGGA
BsiEI 216 CGACCG CG[GA][CT]^CG
BsoBI 307 CCCGAG C^[CT]CG[GA]G
BspEI 356 TCCGGA T^CCGGA
BspHI 191 TCATGA T^CATGA
BspLU11I 917 ACATGT A^CATGT
BsrDI 125 GCAATG GCAATG(2/0)
BstEII 348 GGTCACC G^GT[ACGT]ACC
BstPI 348 GGTCACC G^GT[ACGT]ACC
BstUI 1213 CGCG CG^CG
Bsu36I 156 CCTCAGG CC^T[ACGT]AGG
DraII 1082 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 340 GACGCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 182 TGGCCA [CT]^GGCC[GA]
Eco81I 156 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1082 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 348 GGTCACC G^GT[ACGT]ACC
MluNI 182 TGGCCA TGG^CCA
MroI 356 TCCGGA T^CCGGA
MscI 182 TGGCCA TGG^CCA
MvnI 1213 CGCG CG^CG
NaeI 1162 GCCGGC GCC^GGC
NciI 953 CCGGG CC^[GC]GG
NgoMIV 1162 GCCGGC G^CCGGC
PflFI 430 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1030 CTGCAG CTGCA^G
PvuII 244 CAGCTG CAG^CTG
RcaI 191 TCATGA T^CATGA
SacI 751 GAGCTC GAGCT^C
SfcI 1030 CTGCAG C^T[GA][CT]AG
SphI 497 GCATGC GCATG^C
TaqI 572 TCGA T^CGA
TfiI 705 GATTC G^A[AT]TC
TspRI 336 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 430 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 572 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 715 GTCTAC GT^[AC][GT]AC
1117 GTCTAC
AflIII 917 ACATGT A^C[GA][CT]GT
1199 ACACGT
AlwNI 103 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
163 CAGGAGCTG
BamHI 118 GGATCC G^GATCC
303 GGATCC
BbeI 45 GGCGCC GGCGC^C
1136 GGCGCC
BlpI 225 GCTGAGC GC^T[ACGT]AGC
622 GCTAAGC
Bpu1102I 225 GCTGAGC GC^T[ACGT]AGC
622 GCTAAGC
BsgI 241 GTGCAG GTGCAG(16/14)
250 GTGCAG
BsmBI 435 CGTCTC CGTCTC(1/5)
588 CGTCTC
BsmI 27 GAATGC GAATGC(1/-1)
762 GAATGC
BspMI 206 ACCTGC ACCTGC(4/8)
1028 ACCTGC
BsrFI 362 ACCGGC [GA]^CCGG[CT]
1162 GCCGGC
BstAPI 162 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
243 GCAGCTGGTGC
CelII 225 GCTGAGC GC^T[ACGT]AGC
622 GCTAAGC
Cfr10I 362 ACCGGC [GA]^CCGG[CT]
1162 GCCGGC
FauI 87 CCCGC CCCGC(4/6)
998 CCCGC
KasI 45 GGCGCC G^GCGCC
1136 GGCGCC
MslI 492 CATCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
855 CATCAAGATG
NarI 45 GGCGCC GG^CGCC
1136 GGCGCC
NcoI 386 CCATGG C^CATGG
1043 CCATGG
SapI 753 GCTCTTC GCTCTTC(1/4)
1035 GCTCTTC
ScaI 455 AGTACT AGT^ACT
1013 AGTACT
SfoI 45 GGCGCC GGC^GCC
1136 GGCGCC
XcmI 815 CCATCAAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1091 CCACGGACTCGGTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BanII BbvI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TseI Tsp45I XhoII