(Circular) (Six-base) MAPSORT of: pFN21AE3810.seq Check: 9357 from: 1 to: 5926 pFN21AE3810 5926 bp With 182 enzymes: SgfI * March 28, 2012 12:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 3123 3269 1937 Size: 784 402 146 4594 Fragments arranged by size: 4594 784 402 146 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2056 2899 3989 5373 5641 1632 Size: 416 8 843 1090 1384 268 1917 Fragments arranged by size: 1917 1384 1090 843 416 268 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 2922 3204 4825 5802 1203 Size: 1719 282 1621 977 1327 Fragments arranged by size: 1719 1621 1327 977 282 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 2113 2173 3669 5241 2113 Size: 60 1496 1572 2798 Fragments arranged by size: 2798 1572 1496 60 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2312 3251 1870 Size: 73 369 939 4545 Fragments arranged by size: 4545 939 369 73 BamHI G'GATC_C Cuts at: 2123 2308 3256 3623 2123 Size: 185 948 367 4426 Fragments arranged by size: 4426 948 367 185 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2383 2954 3141 3247 Size: 325 217 627 263 333 571 187 106 Cuts at: 3247 4702 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 571 333 325 263 217 187 106 BanII G_rGCy'C Cuts at: 1391 2760 2994 3198 3245 1391 Size: 1369 234 204 47 4072 Fragments arranged by size: 4072 1369 234 204 47 BbeI G_GCGC'C Cuts at: 2054 3145 2054 Size: 1091 4835 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BbvCI CC'TCA_GC Cuts at: 2483 2483 Size: 5926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4117 4151 1903 Size: 34 50 34 2096 34 3678 Fragments arranged by size: 3678 2096 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2777 4537 136 Size: 107 122 71 631 605 1105 1760 1525 Fragments arranged by size: 1760 1525 1105 631 605 122 107 71 BglII A'GATC_T Cuts at: 2851 5921 2851 Size: 3070 2856 BlpI GC'TnA_GC Cuts at: 2231 2628 3343 2231 Size: 397 715 4814 Fragments arranged by size: 4814 715 397 Bme1580I G_kGCm'C Cuts at: 1790 2388 3186 3987 5143 1790 Size: 598 798 801 1156 2573 Fragments arranged by size: 2573 1156 801 798 598 BmgBI CAC'GTC Cuts at: 1318 2380 3207 1318 Size: 1062 827 4037 Fragments arranged by size: 4037 1062 827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2690 4586 1239 Size: 1451 1896 2579 Fragments arranged by size: 2579 1896 1451 Bpu10I CC'TnA_GC Cuts at: 2330 2483 2330 Size: 153 5773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2429 2914 3068 4051 4916 5214 2429 Size: 485 154 983 865 298 3141 Fragments arranged by size: 3141 983 865 485 298 154 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3024 4589 882 Size: 617 1525 1565 2219 Fragments arranged by size: 2219 1565 1525 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 3717 493 Size: 711 2009 504 2702 Fragments arranged by size: 2702 2009 711 504 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2051 2346 3142 3733 Size: 53 83 186 936 518 295 796 591 Cuts at: 3733 4115 4750 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 796 635 591 518 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 2361 4359 4757 5031 5178 5589 2361 Size: 1998 398 274 147 411 2698 Fragments arranged by size: 2698 1998 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 2714 2744 2912 Size: 30 165 30 1023 30 258 30 168 Cuts at: 2912 2942 1178 Size: 30 4162 Fragments arranged by size: 4162 1023 258 168 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5926 BseYI C'CCAG_C Cuts at: 2010 2548 2946 3185 5129 5625 2010 Size: 538 398 239 1944 496 2311 Fragments arranged by size: 2311 1944 538 496 398 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 2276 1163 Size: 573 531 9 4813 Fragments arranged by size: 4813 573 531 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 3376 4137 5165 1540 Size: 275 186 223 1152 761 1028 2301 Fragments arranged by size: 2301 1152 1028 761 275 223 186 BsiHKAI G_wGCw'C Cuts at: 2070 2237 2760 3987 4072 5143 2070 Size: 167 523 1227 85 1071 2853 Fragments arranged by size: 2853 1227 1071 523 167 85 BsmI GAATG_Cn' Cuts at: 2038 2773 3437 3530 2038 Size: 735 664 93 4434 Fragments arranged by size: 4434 735 664 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2446 2599 2446 Size: 153 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2237 2388 2760 2994 3186 3198 Size: 399 280 167 151 372 234 192 12 Cuts at: 3198 3245 3987 4072 5143 1391 Size: 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 280 234 192 167 151 85 47 12 BspEI T'CCGG_A Cuts at: 2361 5589 2361 Size: 3228 2698 BspHI T'CATG_A Cuts at: 2196 3815 5545 2196 Size: 1619 1730 2577 Fragments arranged by size: 2577 1730 1619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3042 3281 844 Size: 1376 822 239 3489 Fragments arranged by size: 3489 1376 822 239 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 4421 4595 65 Size: 1183 889 2284 174 1396 Fragments arranged by size: 2284 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2367 3167 4570 4757 1700 Size: 110 557 800 1403 187 2869 Fragments arranged by size: 2869 1403 800 557 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2173 2254 794 Size: 1379 81 4466 Fragments arranged by size: 4466 1379 81 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 2353 1184 Size: 1169 4757 BstYI r'GATC_y Cuts at: 1972 2123 2308 2673 2851 3256 3623 4008 4025 Size: 151 185 365 178 405 367 385 17 Cuts at: 4025 5477 5563 5575 5921 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 405 385 367 365 346 185 178 151 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2162 2162 Size: 5926 BtgI C'CryG_G Cuts at: 513 1065 2391 3048 3096 513 Size: 552 1326 657 48 3343 Fragments arranged by size: 3343 1326 657 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2306 2445 487 Size: 683 56 1080 139 3968 Fragments arranged by size: 3968 1080 683 139 56 BtsI GCAGTG_nn' Cuts at: 3442 4236 4256 3442 Size: 794 20 5112 Fragments arranged by size: 5112 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2351 4933 809 Size: 507 1035 2582 1802 Fragments arranged by size: 2582 1802 1035 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 3373 4262 Size: 54 1425 50 275 18 357 1186 889 Cuts at: 4262 5891 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1186 889 357 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2765 2918 3047 3856 1353 Size: 1412 153 129 809 3423 Fragments arranged by size: 3423 1412 809 153 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2758 2758 Size: 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2056 2690 2899 3989 4586 5373 Size: 393 416 8 634 209 1090 597 787 Cuts at: 5373 5641 1239 Size: 268 1524 Fragments arranged by size: 1524 1090 787 634 597 416 393 268 209 8 EcoNI CCTnn'n_nnAGG Cuts at: 2161 2165 2303 2161 Size: 4 138 5784 Fragments arranged by size: 5784 138 4 EcoO109I rG'GnC_Cy Cuts at: 3088 3088 Size: 5926 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2423 2423 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2024 2054 3115 3145 5073 1678 Size: 346 30 1061 30 1928 2531 Fragments arranged by size: 2531 1928 1061 346 30 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2426 2458 4567 Size: 32 135 32 536 32 286 32 2109 Cuts at: 4567 4599 4641 4673 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2109 536 286 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2050 3141 2050 Size: 1091 4835 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 5040 5224 1305 Size: 1164 2571 184 2007 Fragments arranged by size: 2571 2007 1164 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 10 Size: 54 1425 343 357 3747 Fragments arranged by size: 3747 1425 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 2864 3884 4243 4402 518 Size: 648 1335 363 1020 359 159 2042 Fragments arranged by size: 2042 1335 1020 648 363 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2251 2971 3342 4019 5167 5412 1606 Size: 489 156 720 371 677 1148 245 2120 Fragments arranged by size: 2120 1148 720 677 489 371 245 156 NaeI GCC'GGC Cuts at: 3169 3169 Size: 5926 NarI GG'CG_CC Cuts at: 2051 3142 2051 Size: 1091 4835 NcoI C'CATG_G Cuts at: 513 1065 2391 3048 513 Size: 552 1326 657 3391 Fragments arranged by size: 3391 1326 657 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 3167 3167 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2506 2749 2926 3284 4829 1132 Size: 97 1277 243 177 358 1545 2229 Fragments arranged by size: 2229 1545 1277 358 243 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 2922 4825 2922 Size: 1903 4023 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5024 PfoI T'CCnGG_A Cuts at: 2280 2298 2466 2676 3159 2280 Size: 18 168 210 483 5047 Fragments arranged by size: 5047 483 210 168 18 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2444 2476 2444 Size: 32 5894 PstI C_TGCA'G Cuts at: 830 3039 3278 830 Size: 2209 239 3478 Fragments arranged by size: 3478 2209 239 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2251 2251 Size: 5926 SacI G_AGCT'C Cuts at: 2760 2760 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SapI GCTCTTCn'nnn_ Cuts at: 2765 3047 2765 Size: 282 5644 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 2462 3020 4174 5886 2462 Size: 558 1154 1712 2502 Fragments arranged by size: 2502 1712 1154 558 SfcI C'TryA_G Cuts at: 826 1045 3035 3274 4409 5090 5281 826 Size: 219 1990 239 1135 681 191 1471 Fragments arranged by size: 1990 1471 1135 681 239 219 191 SfoI GGC'GCC Cuts at: 2052 3143 2052 Size: 1091 4835 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2893 3083 4030 4931 5193 Size: 197 926 465 485 190 947 901 262 Cuts at: 5193 820 Size: 1553 Fragments arranged by size: 1553 947 926 901 485 465 262 197 190 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 2506 3284 1132 Size: 1374 778 3774 Fragments arranged by size: 3774 1374 778 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2391 2838 3048 3365 513 Size: 552 1326 447 210 317 3074 Fragments arranged by size: 3074 1326 552 447 317 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2002 2238 2831 2833 2889 2953 3080 Size: 1255 422 236 593 2 56 64 127 Cuts at: 3080 3953 3970 4123 4308 4753 325 Size: 873 17 153 185 445 1498 Fragments arranged by size: 1498 1255 873 593 445 422 236 185 153 127 64 56 17 2 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 2412 2460 Size: 274 80 33 51 1443 81 354 48 Cuts at: 2460 2925 3018 4172 5884 96 Size: 465 93 1154 1712 138 Fragments arranged by size: 1712 1443 1154 465 354 274 138 93 81 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 2438 1316 Size: 1122 4804 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2827 3103 2827 Size: 276 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI