(Linear) (Six-base) MAPSORT of: KIEE3810.seq Check: 6511 from: 1 to: 1221 KIEE3810.orf With 182 enzymes: SgfI * March 22, 2012 16:46 .. AccI GT'mk_AC Cuts at: 0 716 1118 1221 Size: 716 402 103 Fragments arranged by size: 716 402 103 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 51 894 1221 Size: 43 8 843 327 Fragments arranged by size: 843 327 43 8 AflIII A'CryG_T Cuts at: 0 917 1199 1221 Size: 917 282 22 Fragments arranged by size: 917 282 22 AlwNI CAG_nnn'CTG Cuts at: 0 108 168 1221 Size: 108 60 1053 Fragments arranged by size: 1053 108 60 AvaI C'yCGr_G Cuts at: 0 307 1221 Size: 307 914 BamHI G'GATC_C Cuts at: 0 118 303 1221 Size: 118 185 918 Fragments arranged by size: 918 185 118 BanI G'GyrC_C Cuts at: 0 45 378 949 1136 1221 Size: 45 333 571 187 85 Fragments arranged by size: 571 333 187 85 45 BanII G_rGCy'C Cuts at: 0 755 989 1193 1221 Size: 755 234 204 28 Fragments arranged by size: 755 234 204 28 BbeI G_GCGC'C Cuts at: 0 49 1140 1221 Size: 49 1091 81 Fragments arranged by size: 1091 81 49 BbvCI CC'TCA_GC Cuts at: 0 478 1221 Size: 478 743 BglI GCCn_nnn'nGGC Cuts at: 0 772 1221 Size: 772 449 BglII A'GATC_T Cuts at: 0 846 1221 Size: 846 375 BlpI GC'TnA_GC Cuts at: 0 226 623 1221 Size: 226 397 598 Fragments arranged by size: 598 397 226 Bme1580I G_kGCm'C Cuts at: 0 383 1181 1221 Size: 383 798 40 Fragments arranged by size: 798 383 40 BmgBI CAC'GTC Cuts at: 0 375 1202 1221 Size: 375 827 19 Fragments arranged by size: 827 375 19 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 159 1221 Size: 159 1062 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 1221 Size: 685 536 Bpu10I CC'TnA_GC Cuts at: 0 325 478 1221 Size: 325 153 743 Fragments arranged by size: 743 325 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 909 1063 1221 Size: 424 485 154 158 Fragments arranged by size: 485 424 158 154 BsaI GGTCTCn'nnnn_ Cuts at: 0 1019 1221 Size: 1019 202 BsaAI yAC'GTr Cuts at: 0 1208 1221 Size: 1208 13 BsaHI Gr'CG_yC Cuts at: 0 46 341 1137 1221 Size: 46 295 796 84 Fragments arranged by size: 796 295 84 46 BsaWI w'CCGG_w Cuts at: 0 356 1221 Size: 356 865 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 421 451 709 739 907 937 1221 Size: 421 30 258 30 168 30 284 Fragments arranged by size: 421 284 258 168 30 30 30 BseYI C'CCAG_C Cuts at: 0 5 543 941 1180 1221 Size: 5 538 398 239 41 Fragments arranged by size: 538 398 239 41 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 271 1221 Size: 262 9 950 Fragments arranged by size: 950 262 9 BsiEI CG_ry'CG Cuts at: 0 219 1221 Size: 219 1002 BsiHKAI G_wGCw'C Cuts at: 0 65 232 755 1221 Size: 65 167 523 466 Fragments arranged by size: 523 466 167 65 BsmI GAATG_Cn' Cuts at: 0 33 768 1221 Size: 33 735 453 Fragments arranged by size: 735 453 33 BsmBI CGTCTCn'nnnn_ Cuts at: 0 441 594 1221 Size: 441 153 627 Fragments arranged by size: 627 441 153 Bsp1286I G_dGCh'C Cuts at: 0 65 232 383 755 989 1181 1193 1221 Size: 65 167 151 372 234 192 12 28 Fragments arranged by size: 372 234 192 167 151 65 28 12 BspEI T'CCGG_A Cuts at: 0 356 1221 Size: 356 865 BspHI T'CATG_A Cuts at: 0 191 1221 Size: 191 1030 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 215 1037 1221 Size: 215 822 184 Fragments arranged by size: 822 215 184 BsrDI GCAATG_nn' Cuts at: 0 132 1221 Size: 132 1089 BsrFI r'CCGG_y Cuts at: 0 362 1162 1221 Size: 362 800 59 Fragments arranged by size: 800 362 59 BstAPI GCAn_nnn'nTGC Cuts at: 0 168 249 1221 Size: 168 81 972 Fragments arranged by size: 972 168 81 BstEII G'GTnAC_C Cuts at: 0 348 1221 Size: 348 873 BstYI r'GATC_y Cuts at: 0 118 303 668 846 1221 Size: 118 185 365 178 375 Fragments arranged by size: 375 365 185 178 118 Bsu36I CC'TnA_GG Cuts at: 0 157 1221 Size: 157 1064 BtgI C'CryG_G Cuts at: 0 386 1043 1091 1221 Size: 386 657 48 130 Fragments arranged by size: 657 386 130 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 301 440 1221 Size: 301 139 781 Fragments arranged by size: 781 301 139 DrdI GACnn_nn'nnGTC Cuts at: 0 346 1221 Size: 346 875 EaeI y'GGCC_r Cuts at: 0 182 1221 Size: 182 1039 EarI CTCTTCn'nnn_ Cuts at: 0 760 913 1042 1221 Size: 760 153 129 179 Fragments arranged by size: 760 179 153 129 EcoICRI GAG'CTC Cuts at: 0 753 1221 Size: 753 468 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 51 685 894 1221 Size: 43 8 634 209 327 Fragments arranged by size: 634 327 209 43 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 156 160 298 1221 Size: 156 4 138 923 Fragments arranged by size: 923 156 138 4 EcoO109I rG'GnC_Cy Cuts at: 0 1083 1221 Size: 1083 138 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 418 1221 Size: 418 803 HaeII r_GCGC'y Cuts at: 0 19 49 1110 1140 1221 Size: 19 30 1061 30 81 Fragments arranged by size: 1061 81 30 30 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 421 453 1221 Size: 103 32 286 32 768 Fragments arranged by size: 768 286 103 32 32 KasI G'GCGC_C Cuts at: 0 45 1136 1221 Size: 45 1091 85 Fragments arranged by size: 1091 85 45 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 464 1221 Size: 464 757 MscI TGG'CCA Cuts at: 0 184 1221 Size: 184 1037 MslI CAynn'nnrTG Cuts at: 0 496 859 1221 Size: 496 363 362 Fragments arranged by size: 496 363 362 MspA1I CmG'CkG Cuts at: 0 90 246 966 1221 Size: 90 156 720 255 Fragments arranged by size: 720 255 156 90 NaeI GCC'GGC Cuts at: 0 1164 1221 Size: 1164 57 NarI GG'CG_CC Cuts at: 0 46 1137 1221 Size: 46 1091 84 Fragments arranged by size: 1091 84 46 NcoI C'CATG_G Cuts at: 0 386 1043 1221 Size: 386 657 178 Fragments arranged by size: 657 386 178 NgoMIV G'CCGG_C Cuts at: 0 1162 1221 Size: 1162 59 NspI r_CATG'y Cuts at: 0 501 744 921 1221 Size: 501 243 177 300 Fragments arranged by size: 501 300 243 177 PciI A'CATG_T Cuts at: 0 917 1221 Size: 917 304 PfoI T'CCnGG_A Cuts at: 0 275 293 461 671 1154 1221 Size: 275 18 168 210 483 67 Fragments arranged by size: 483 275 210 168 67 18 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 439 471 1221 Size: 439 32 750 Fragments arranged by size: 750 439 32 PstI C_TGCA'G Cuts at: 0 1034 1221 Size: 1034 187 PvuII CAG'CTG Cuts at: 0 246 1221 Size: 246 975 SacI G_AGCT'C Cuts at: 0 755 1221 Size: 755 466 SapI GCTCTTCn'nnn_ Cuts at: 0 760 1042 1221 Size: 760 282 179 Fragments arranged by size: 760 282 179 ScaI AGT'ACT Cuts at: 0 457 1015 1221 Size: 457 558 206 Fragments arranged by size: 558 457 206 SfcI C'TryA_G Cuts at: 0 1030 1221 Size: 1030 191 SfoI GGC'GCC Cuts at: 0 47 1138 1221 Size: 47 1091 83 Fragments arranged by size: 1091 83 47 SmlI C'TyrA_G Cuts at: 0 403 888 1078 1221 Size: 403 485 190 143 Fragments arranged by size: 485 403 190 143 SphI G_CATG'C Cuts at: 0 501 1221 Size: 501 720 StyI C'CwwG_G Cuts at: 0 386 833 1043 1221 Size: 386 447 210 178 Fragments arranged by size: 447 386 210 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 233 826 828 884 948 1075 1221 Size: 233 593 2 56 64 127 146 Fragments arranged by size: 593 233 146 127 64 56 2 TatI w'GTAC_w Cuts at: 0 53 407 455 920 1013 1221 Size: 53 354 48 465 93 208 Fragments arranged by size: 465 354 208 93 53 48 Tth111I GACn'n_nGTC Cuts at: 0 433 1221 Size: 433 788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 822 1098 1221 Size: 822 276 123 Fragments arranged by size: 822 276 123 Enzymes that do cut: AccI AcuI AflIII AlwNI AvaI BamHI BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtgZI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PsrI PstI PvuII SacI SapI ScaI SfcI SfoI SmlI SphI StyI TaqII TatI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BmrI BmtI BsaBI BseRI BsiWI BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtsI ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI XhoI XmaI XmnI ZraI