################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BfrI BglII BlnI BpuAI BsaAI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII EaeI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT14I FseI FspI HgaI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 299 GTCTAC GT^[AC][GT]AC AccIII 305 TCCGGA T^CCGGA AcyI 37 GGCGCC G[GA]^CG[CT]C AhdI 925 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 262 AGCT AG^CT AlwI 308 GGATC GGATC(4/5) AocI 1053 CCTGAGG CC^T[ACGT]AGG AspEI 925 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 625 GTGCTC G[AT]GC[AT]^C BanI 37 GGCGCC G^G[CT][GA]CC BbeI 37 GGCGCC GGCGC^C BbvCI 209 CCTCAGC CCTCAGC(-5/-2) BciVI 728 GTATCC GTATCC(6/5) BclI 401 TGATCA T^GATCA BfaI 574 CTAG C^TAG BsaHI 37 GGCGCC G[GA]^CG[CT]C BsaI 739 GAGACC GGTCTC(1/5) BsaWI 305 TCCGGA [AT]^CCGG[AT] BseAI 305 TCCGGA T^CCGGA BsgI 868 CTGCAC GTGCAG(16/14) BsiHKAI 625 GTGCTC G[AT]GC[AT]^C BsmI 993 GAATGC GAATGC(1/-1) BspEI 305 TCCGGA T^CCGGA BspMI 591 ACCTGC ACCTGC(4/8) BsrBI 177 GAGCGG CCGCTC(-3/-3) BsrDI 548 GCAATG GCAATG(2/0) BssSI 736 CACGAG CACGAG(-5/-1) Bsu36I 1053 CCTGAGG CC^T[ACGT]AGG DrdI 953 GACTGATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 222 CCACGG C^C[GA][CT]GG Eam1105I 925 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1053 CCTGAGG CC^T[ACGT]AGG EcoRV 319 GATATC GAT^ATC EcoT22I 395 ATGCAT ATGCA^T FbaI 401 TGATCA T^GATCA HaeII 37 GGCGCC [GA]GCGC^[CT] Hin1I 37 GGCGCC G[GA]^CG[CT]C HincII 376 GTTGAC GT[CT]^[GA]AC HindII 376 GTTGAC GT[CT]^[GA]AC HphI 807 GGTGA GGTGA(8/7) KasI 37 GGCGCC G^GCGCC MaeI 574 CTAG C^TAG MaeII 960 ACGT A^CGT MaeIII 761 GTGAC ^GT[ACGT]AC MboII 440 TCTTC GAAGA(8/7) MroI 305 TCCGGA T^CCGGA MseI 530 TTAA T^TAA NarI 37 GGCGCC GG^CGCC NsiI 395 ATGCAT ATGCA^T SfoI 37 GGCGCC GGC^GCC SmlI 564 CTTGAG C^T[CT][GA]AG TfiI 390 GATTC G^A[AT]TC Tru9I 530 TTAA T^TAA Tsp45I 761 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccII 231 CGCG CG^CG 288 CGCG AfaI 364 GTAC GT^AC 578 GTAC AlwNI 212 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 640 CAGTCCCTG ApaI 51 GGGCCC GGGCC^C 168 GGGCCC BglI 40 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 220 GCCCACGGGGC BlpI 237 GCTTAGC GC^T[ACGT]AGC 258 GCTCAGC Bpu1102I 237 GCTTAGC GC^T[ACGT]AGC 258 GCTCAGC BstUI 231 CGCG CG^CG 288 CGCG CelII 237 GCTTAGC GC^T[ACGT]AGC 258 GCTCAGC EcoNI 343 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 424 CCTTATGAAGG MvnI 231 CGCG CG^CG 288 CGCG PleI 1006 GACTC GAGTC(4/5) 9 GAGTC PpuMI 248 GGGACCC [GA]G^G[AT]CC[CT] 893 AGGACCC RsaI 364 GTAC GT^AC 578 GTAC SfaNI 942 GATGC GCATC(5/9) 614 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SmaI TseI TspRI XmaCI XmaI