(Circular) (Six-base) MAPSORT of: pFN21AE3144.seq Check: 3373 from: 1 to: 5767 pFN21AE3144 5767 bp With 182 enzymes: SgfI * March 28, 2012 13:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2305 3110 1937 Size: 368 805 4594 Fragments arranged by size: 4594 805 368 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3830 5214 5482 1632 Size: 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 4666 5643 1203 Size: 3463 977 1327 Fragments arranged by size: 3463 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 2935 4496 2935 Size: 1561 4206 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 2222 2650 3510 5082 2222 Size: 428 860 1572 2907 Fragments arranged by size: 2907 1572 860 428 ApaI G_GGCC'C Cuts at: 2060 2177 2060 Size: 117 5650 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2176 2201 2493 3092 1870 Size: 73 117 116 25 292 599 4545 Fragments arranged by size: 4545 599 292 117 116 73 25 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 3088 4543 618 Size: 325 217 627 255 1046 1455 1842 Fragments arranged by size: 1842 1455 1046 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 2060 2177 2208 2609 2682 3086 1391 Size: 669 117 31 401 73 404 4072 Fragments arranged by size: 4072 669 404 401 117 73 31 BbeI G_GCGC'C Cuts at: 2046 2046 Size: 5767 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BbvCI CC'TCA_GC Cuts at: 2215 2215 Size: 5767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2744 3659 4869 2744 Size: 915 1210 3642 Fragments arranged by size: 3642 1210 915 BclI T'GATC_A Cuts at: 1548 2406 1548 Size: 858 4909 BfrBI ATG'CAT Cuts at: 2402 2402 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2231 4378 Size: 107 122 71 631 605 379 180 2147 Cuts at: 4378 136 Size: 1525 Fragments arranged by size: 2147 1525 631 605 379 180 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 2243 2264 3184 2243 Size: 21 920 4826 Fragments arranged by size: 4826 920 21 Bme1580I G_kGCm'C Cuts at: 1790 2060 2177 3828 4984 1790 Size: 270 117 1651 1156 2573 Fragments arranged by size: 2573 1651 1156 270 117 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 2284 3498 Size: 416 53 83 399 24 843 24 1214 Cuts at: 3498 4456 442 Size: 958 1753 Fragments arranged by size: 1753 1214 958 843 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 2623 2852 2999 4427 1239 Size: 1098 286 229 147 1428 2579 Fragments arranged by size: 2579 1428 1098 286 229 147 Bpu10I CC'TnA_GC Cuts at: 2215 2215 Size: 5767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2590 3892 4757 5055 2590 Size: 1302 865 298 3302 Fragments arranged by size: 3302 1302 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 4430 882 Size: 617 1239 1692 2219 Fragments arranged by size: 2219 1692 1239 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 3574 3956 4591 Size: 53 83 186 936 510 1531 382 635 Cuts at: 4591 275 Size: 1451 Fragments arranged by size: 1531 1451 936 635 510 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2310 4200 4598 4872 5019 5430 2310 Size: 1890 398 274 147 411 2647 Fragments arranged by size: 2647 1890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5542 Fragments arranged by size: 5542 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2340 2614 1186 Size: 853 301 274 4339 Fragments arranged by size: 4339 853 301 274 BseYI C'CCAG_C Cuts at: 2608 4970 5466 2608 Size: 2362 496 2909 Fragments arranged by size: 2909 2362 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2858 1163 Size: 573 1122 4072 Fragments arranged by size: 4072 1122 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2634 3828 3913 4984 2634 Size: 1194 85 1071 3417 Fragments arranged by size: 3417 1194 1071 85 BsmI GAATG_Cn' Cuts at: 3004 3278 3371 3004 Size: 274 93 5400 Fragments arranged by size: 5400 274 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2177 2208 2609 2634 2682 3086 Size: 399 270 117 31 401 25 48 404 Cuts at: 3086 3828 3913 4984 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 404 401 399 270 117 85 48 31 25 BspEI T'CCGG_A Cuts at: 2310 5430 2310 Size: 3120 2647 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2605 3122 844 Size: 1761 517 3489 Fragments arranged by size: 3489 1761 517 BsrBI CCG'CTC Cuts at: 1394 1823 2184 3654 1394 Size: 429 361 1470 3507 Fragments arranged by size: 3507 1470 429 361 BsrDI GCAATG_nn' Cuts at: 65 1248 2560 4262 4436 65 Size: 1183 1312 1702 174 1396 Fragments arranged by size: 1702 1396 1312 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4411 4598 1700 Size: 110 2601 187 2869 Fragments arranged by size: 2869 2601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 2741 3827 4839 2741 Size: 1086 1012 3669 Fragments arranged by size: 3669 1086 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstYI r'GATC_y Cuts at: 1972 3097 3464 3849 3866 5318 5404 5416 5762 Size: 1125 367 385 17 1452 86 12 346 Cuts at: 5762 1972 Size: 1977 Fragments arranged by size: 1977 1452 1125 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3059 3059 Size: 5767 BtgI C'CryG_G Cuts at: 513 1065 2004 2227 513 Size: 552 939 223 4053 Fragments arranged by size: 4053 939 552 223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2306 3283 4077 4097 2306 Size: 977 794 20 3976 Fragments arranged by size: 3976 977 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2964 4774 809 Size: 507 1648 1810 1802 Fragments arranged by size: 1810 1802 1648 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3214 4103 5732 Size: 54 1425 50 275 18 1384 889 1629 Cuts at: 5732 8 Size: 43 Fragments arranged by size: 1629 1425 1384 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3697 1353 Size: 2344 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2457 4354 4728 4874 387 Size: 1064 138 435 433 1897 374 146 1280 Fragments arranged by size: 1897 1280 1064 435 433 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2623 2852 2999 3830 4427 5214 Size: 393 705 286 229 147 831 597 787 Cuts at: 5214 5482 1239 Size: 268 1524 Fragments arranged by size: 1524 831 787 705 597 393 286 268 229 147 EcoNI CCTnn'n_nnAGG Cuts at: 2352 2433 2352 Size: 81 5686 EcoO109I rG'GnC_Cy Cuts at: 2057 2173 2254 2899 2057 Size: 116 81 645 4925 Fragments arranged by size: 4925 645 116 81 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 EcoRV GAT'ATC Cuts at: 2326 2326 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2046 4914 1678 Size: 368 2868 2531 Fragments arranged by size: 2868 2531 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2745 2777 2921 Size: 32 135 32 743 32 398 32 144 Cuts at: 2921 2953 4408 4440 4482 4514 1373 Size: 32 1455 32 42 32 2626 Fragments arranged by size: 2626 1455 743 398 144 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 3111 3357 669 Size: 988 281 445 728 246 3079 Fragments arranged by size: 3079 988 728 445 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KasI G'GCGC_C Cuts at: 2042 2042 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2042 2295 4881 5065 1305 Size: 737 253 2586 184 2007 Fragments arranged by size: 2586 2007 737 253 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3945 Fragments arranged by size: 3945 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3725 4084 4243 518 Size: 648 2559 359 159 2042 Fragments arranged by size: 2559 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2103 2159 2197 2222 3183 3860 5008 5253 Size: 497 56 38 25 961 677 1148 245 Cuts at: 5253 1606 Size: 2120 Fragments arranged by size: 2120 1148 961 677 497 245 56 38 25 NarI GG'CG_CC Cuts at: 2043 2043 Size: 5767 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4276 Fragments arranged by size: 4276 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NsiI A_TGCA'T Cuts at: 2404 2404 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 3125 4670 1132 Size: 97 1896 1545 2229 Fragments arranged by size: 2229 1896 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PfoI T'CCnGG_A Cuts at: 2353 2704 2353 Size: 351 5416 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5349 5381 3836 Size: 32 1481 32 4222 Fragments arranged by size: 4222 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2899 2254 Size: 645 5122 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 2340 3337 2340 Size: 997 4770 PspOMI G'GGCC_C Cuts at: 2056 2173 2056 Size: 117 5650 PstI C_TGCA'G Cuts at: 830 3119 830 Size: 2289 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SanDI GG'GwC_CC Cuts at: 2254 2254 Size: 5767 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 3115 4250 4931 5122 826 Size: 219 2070 1135 681 191 1471 Fragments arranged by size: 2070 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2044 2044 Size: 5767 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 2062 2178 2203 3094 1872 Size: 190 116 25 891 4545 Fragments arranged by size: 4545 891 190 116 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 2569 3871 4772 5034 820 Size: 197 926 626 1302 901 262 1553 Fragments arranged by size: 1553 1302 926 901 626 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SrfI GCCC'GGGC Cuts at: 2203 2203 Size: 5767 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2004 3206 513 Size: 552 939 1202 3074 Fragments arranged by size: 3074 1202 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2502 3794 3811 3964 4149 4594 325 Size: 1255 922 1292 17 153 185 445 1498 Fragments arranged by size: 1498 1292 1255 922 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 5725 96 Size: 274 80 33 51 1443 2036 1712 138 Fragments arranged by size: 2036 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 5727 5759 2768 Size: 32 2927 32 2776 Fragments arranged by size: 2927 2776 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 2060 2176 2201 3092 1870 Size: 190 116 25 891 4545 Fragments arranged by size: 4545 891 190 116 25 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SwaI XcmI