################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BbvI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsgI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI MaeI MaeII MamI MfeI MluI MluNI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TseI Tsp45I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 217 GTCTAC GT^[AC][GT]AC AccIII 371 TCCGGA T^CCGGA AcsI 463 GAATTT [GA]^AATT[CT] AfaI 174 GTAC GT^AC AlwI 374 GGATC GGATC(4/5) ApaI 597 GGGCCC GGGCC^C ApoI 463 GAATTT [GA]^AATT[CT] AvaII 605 GGACC G^G[AT]CC BanI 325 GGCGCC G^G[CT][GA]CC BbeI 325 GGCGCC GGCGC^C BcgI 500 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 313 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 371 TCCGGA T^CCGGA BseRI 634 GAGGAG GAGGAG(10/8) BsmI 21 GAATGC GAATGC(1/-1) BspEI 371 TCCGGA T^CCGGA BsrBI 131 GAGCGG CCGCTC(-3/-3) BssHII 266 GCGCGC G^CGCGC BstF5I 386 GGATG GGATG(2/0) BstYI 374 GGATCT [GA]^GATC[CT] DdeI 396 CTCAG C^T[ACGT]AG DsaI 593 CCGCGG C^C[GA][CT]GG FokI 386 GGATG GGATG(9/13) KasI 325 GGCGCC G^GCGCC KspI 593 CCGCGG CCGC^GG MaeIII 365 GTAAC ^GT[ACGT]AC MflI 374 GGATCT [GA]^GATC[CT] MroI 371 TCCGGA T^CCGGA MspA1I 593 CCGCGG C[AC]G^C[GT]G NarI 325 GGCGCC GG^CGCC NspI 257 ACATGC [GA]CATG^[CT] PaeR7I 238 CTCGAG C^TCGAG PflMI 441 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 174 GTAC GT^AC SacII 593 CCGCGG CCGC^GG SfoI 325 GGCGCC GGC^GCC Tsp509I 464 AATT ^AATT TspRI 262 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 441 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 238 CTCGAG C^TCGAG XhoII 374 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 214 GGCGTC G[GA]^CG[CT]C 325 GGCGCC AluI 394 AGCT AG^CT 452 AGCT AlwNI 398 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 442 CAGACCCTG BanII 235 GGGCTC G[GA]GC[CT]^C 597 GGGCCC BmyI 235 GGGCTC G[AGT]GC[ACT]^C 597 GGGCCC BpmI 448 CTGGAG CTGGAG(16/14) 454 CTGGAG BsaHI 214 GGCGTC G[GA]^CG[CT]C 325 GGCGCC BsaWI 67 ACCGGA [AT]^CCGG[AT] 371 TCCGGA Bsp1286I 235 GGGCTC G[AGT]GC[ACT]^C 597 GGGCCC BstNI 383 CCTGG CC^[AT]GG 447 CCTGG EarI 640 GAAGAG CTCTTC(1/4) 646 GAAGAG EcoRII 383 CCTGG ^CC[AT]GG 447 CCTGG EcoT14I 314 CCAAGG C^C[AT][AT]GG 329 CCAAGG HgaI 486 GACGC GACGC(5/10) 215 GCGTC Hin1I 214 GGCGTC G[GA]^CG[CT]C 325 GGCGCC HinfI 307 GACTC G^A[ACGT]TC 343 GACTC Ksp632I 640 GAAGAG CTCTTC(1/4) 646 GAAGAG MboII 640 GAAGA GAAGA(8/7) 646 GAAGA MvaI 383 CCTGG CC^[AT]GG 447 CCTGG NaeI 211 GCCGGC GCC^GGC 226 GCCGGC NgoMIV 211 GCCGGC G^CCGGC 226 GCCGGC PleI 307 GACTC GAGTC(4/5) 343 GACTC SmaI 120 CCCGGG CCC^GGG 600 CCCGGG SmlI 48 CTCAAG C^T[CT][GA]AG 238 CTCGAG StyI 314 CCAAGG C^C[AT][AT]GG 329 CCAAGG XmaCI 120 CCCGGG C^CCGGG 600 CCCGGG XmaI 120 CCCGGG C^CCGGG 600 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AvaI BcnI BsaJI BsiEI BsiYI BslI BsmFI BsoBI BsrFI BssKI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII EaeI EagI EclXI Eco52I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboI MnlI MspI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TthHB8I