(Circular) (Six-base) MAPSORT of: pFN21AB8893.seq Check: 8549 from: 1 to: 5356 pFN21AB8893 5356 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2223 2699 1937 Size: 286 476 4594 Fragments arranged by size: 4594 476 286 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2652 3419 4803 5071 1632 Size: 1020 767 1384 268 1917 Fragments arranged by size: 1917 1384 1020 767 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 2408 2452 3099 4671 2408 Size: 44 647 1572 3093 Fragments arranged by size: 3093 1572 647 44 ApaI G_GGCC'C Cuts at: 2606 2606 Size: 5356 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2468 2665 2891 3043 5152 1493 Size: 975 197 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2125 2243 2605 2681 1870 Size: 73 182 118 362 76 4545 Fragments arranged by size: 4545 362 182 118 76 73 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 2677 4132 618 Size: 325 217 627 543 347 1455 1842 Fragments arranged by size: 1842 1455 627 543 347 325 217 BanII G_rGCy'C Cuts at: 1391 2244 2606 2675 1391 Size: 853 362 69 4072 Fragments arranged by size: 4072 853 362 69 BbeI G_GCGC'C Cuts at: 2334 2334 Size: 5356 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2494 2528 3547 3581 1903 Size: 34 557 34 1019 34 3678 Fragments arranged by size: 3678 1019 557 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 1548 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 3967 136 Size: 107 122 71 631 605 652 1643 1525 Fragments arranged by size: 1643 1525 652 631 605 122 107 71 BglII A'GATC_T Cuts at: 5351 5351 Size: 5356 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 2606 3417 4573 1790 Size: 816 811 1156 2573 Fragments arranged by size: 2573 1156 816 811 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2480 4016 1239 Size: 1235 6 1536 2579 Fragments arranged by size: 2579 1536 1235 6 Bpu10I CC'TnA_GC Cuts at: 2401 2401 Size: 5356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2038 3481 4346 4644 2038 Size: 1443 865 298 2750 Fragments arranged by size: 2750 1443 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2220 2331 3163 3545 Size: 53 83 186 936 687 111 832 382 Cuts at: 3545 4180 275 Size: 635 1451 Fragments arranged by size: 1451 936 832 687 635 382 186 111 83 53 BsaWI w'CCGG_w Cuts at: 2072 2376 3789 4187 4461 4608 5019 2072 Size: 304 1413 398 274 147 411 2409 Fragments arranged by size: 2409 1413 411 398 304 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 1186 Size: 1468 3888 BseYI C'CCAG_C Cuts at: 2118 4559 5055 2118 Size: 2441 496 2419 Fragments arranged by size: 2441 2419 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4783 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2217 2232 2364 2806 3567 4595 Size: 275 186 216 15 132 442 761 1028 Cuts at: 4595 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 442 275 216 186 132 15 BsiHKAI G_wGCw'C Cuts at: 3417 3502 4573 3417 Size: 85 1071 4200 Fragments arranged by size: 4200 1071 85 BsmI GAATG_Cn' Cuts at: 2032 2867 2960 2032 Size: 835 93 4428 Fragments arranged by size: 4428 835 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2606 2675 3417 3502 4573 1391 Size: 399 454 362 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 362 85 69 BspEI T'CCGG_A Cuts at: 2376 5019 2376 Size: 2643 2713 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2711 844 Size: 1867 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2138 3243 1394 Size: 429 315 1105 3507 Fragments arranged by size: 3507 1105 429 315 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2216 2231 2565 2615 4000 4187 1700 Size: 110 406 15 334 50 1385 187 2869 Fragments arranged by size: 2869 1385 406 334 187 110 50 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 2271 1925 Size: 346 5010 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2379 2686 3053 3438 3455 4907 4993 5005 Size: 407 307 367 385 17 1452 86 12 Cuts at: 5005 5351 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 407 385 367 346 307 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2598 513 Size: 552 1533 3271 Fragments arranged by size: 3271 1533 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2266 2872 3666 3686 2266 Size: 606 794 20 3936 Fragments arranged by size: 3936 794 606 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2229 2361 Size: 54 1425 50 275 18 384 15 132 Cuts at: 2361 2617 2803 3692 5321 8 Size: 256 186 889 1629 43 Fragments arranged by size: 1629 1425 889 384 275 256 186 132 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2214 2229 2361 2803 1537 Size: 275 402 15 132 442 4090 Fragments arranged by size: 4090 442 402 275 132 15 EarI CTCTTCn'nnn_ Cuts at: 1353 2640 2646 3286 1353 Size: 1287 6 640 3423 Fragments arranged by size: 3423 1287 640 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2305 3943 4317 4463 387 Size: 1064 138 716 1638 374 146 1280 Fragments arranged by size: 1638 1280 1064 716 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2480 2652 3419 4016 4803 5071 Size: 393 842 6 172 767 597 787 268 Cuts at: 5071 1239 Size: 1524 Fragments arranged by size: 1524 842 787 767 597 393 268 172 6 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 2125 2151 2334 4503 1678 Size: 447 26 183 2169 2531 Fragments arranged by size: 2531 2169 447 183 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373 Size: 32 135 32 2425 32 42 32 2626 Fragments arranged by size: 2626 2425 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KasI G'GCGC_C Cuts at: 2330 2330 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 4470 4654 1305 Size: 831 2334 184 2007 Fragments arranged by size: 2334 2007 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3314 3673 3832 518 Size: 648 2148 359 159 2042 Fragments arranged by size: 2148 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2600 2772 3449 4597 4842 1606 Size: 994 172 677 1148 245 2120 Fragments arranged by size: 2120 1148 994 677 245 172 NaeI GCC'GGC Cuts at: 2218 2233 2218 Size: 15 5341 NarI GG'CG_CC Cuts at: 2331 2331 Size: 5356 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4804 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NgoMIV G'CCGG_C Cuts at: 2216 2231 2216 Size: 15 5341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2266 2714 4259 1132 Size: 97 1037 448 1545 2229 Fragments arranged by size: 2229 1545 1037 448 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 1108 Size: 1344 4012 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PspOMI G'GGCC_C Cuts at: 2602 2602 Size: 5356 PspXI vC'TCGA_Gb Cuts at: 2243 2243 Size: 5356 PstI C_TGCA'G Cuts at: 830 2708 830 Size: 1878 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 SacII CC_GC'GG Cuts at: 2601 2601 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2704 3839 4520 4711 826 Size: 219 1659 1135 681 191 1471 Fragments arranged by size: 1659 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2332 2332 Size: 5356 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2127 2607 2683 1872 Size: 255 480 76 4545 Fragments arranged by size: 4545 480 255 76 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 2243 3460 4361 4623 820 Size: 197 926 110 190 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 262 197 190 110 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SrfI GCCC'GGGC Cuts at: 2127 2127 Size: 5356 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2319 2334 2795 513 Size: 552 1254 15 461 3074 Fragments arranged by size: 3074 1254 552 461 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3383 3400 3553 3738 4183 325 Size: 1255 1803 17 153 185 445 1498 Fragments arranged by size: 1803 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3602 5314 96 Size: 274 80 33 51 1443 1625 1712 138 Fragments arranged by size: 1712 1625 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 2243 1943 Size: 300 5056 XmaI C'CCGG_G Cuts at: 1870 2125 2605 2681 1870 Size: 255 480 76 4545 Fragments arranged by size: 4545 480 255 76 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI