(Linear) (Six-base) MAPSORT of: KIBB8893.seq Check: 5630 from: 1 to: 651 KIBB8893.orf With 182 enzymes: SgfI * January 29, 2014 11:43 .. AccI GT'mk_AC Cuts at: 0 218 651 Size: 218 433 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 647 651 Size: 647 4 AlwNI CAG_nnn'CTG Cuts at: 0 403 447 651 Size: 403 44 204 Fragments arranged by size: 403 204 44 ApaI G_GGCC'C Cuts at: 0 601 651 Size: 601 50 ApoI r'AATT_y Cuts at: 0 463 651 Size: 463 188 AvaI C'yCGr_G Cuts at: 0 120 238 600 651 Size: 120 118 362 51 Fragments arranged by size: 362 120 118 51 BanI G'GyrC_C Cuts at: 0 325 651 Size: 325 326 BanII G_rGCy'C Cuts at: 0 239 601 651 Size: 239 362 50 Fragments arranged by size: 362 239 50 BbeI G_GCGC'C Cuts at: 0 329 651 Size: 329 322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 489 523 651 Size: 489 34 128 Fragments arranged by size: 489 128 34 BglI GCCn_nnn'nGGC Cuts at: 0 319 651 Size: 319 332 Bme1580I G_kGCm'C Cuts at: 0 601 651 Size: 601 50 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 475 651 Size: 469 6 176 Fragments arranged by size: 469 176 6 Bpu10I CC'TnA_GC Cuts at: 0 396 651 Size: 396 255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 651 Size: 33 618 BsaHI Gr'CG_yC Cuts at: 0 215 326 651 Size: 215 111 325 Fragments arranged by size: 325 215 111 BsaWI w'CCGG_w Cuts at: 0 67 371 651 Size: 67 304 280 Fragments arranged by size: 304 280 67 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 649 651 Size: 649 2 BseYI C'CCAG_C Cuts at: 0 113 651 Size: 113 538 BsiEI CG_ry'CG Cuts at: 0 212 227 359 651 Size: 212 15 132 292 Fragments arranged by size: 292 212 132 15 BsmI GAATG_Cn' Cuts at: 0 27 651 Size: 27 624 Bsp1286I G_dGCh'C Cuts at: 0 239 601 651 Size: 239 362 50 Fragments arranged by size: 362 239 50 BspEI T'CCGG_A Cuts at: 0 371 651 Size: 371 280 BsrBI CCG'CTC Cuts at: 0 133 651 Size: 133 518 BsrFI r'CCGG_y Cuts at: 0 211 226 560 610 651 Size: 211 15 334 50 41 Fragments arranged by size: 334 211 50 41 15 BssHII G'CGCG_C Cuts at: 0 266 651 Size: 266 385 BstYI r'GATC_y Cuts at: 0 374 651 Size: 374 277 BtgI C'CryG_G Cuts at: 0 593 651 Size: 593 58 BtsI GCAGTG_nn' Cuts at: 0 261 651 Size: 261 390 EaeI y'GGCC_r Cuts at: 0 209 224 356 612 651 Size: 209 15 132 256 39 Fragments arranged by size: 256 209 132 39 15 EagI C'GGCC_G Cuts at: 0 209 224 356 651 Size: 209 15 132 295 Fragments arranged by size: 295 209 132 15 EarI CTCTTCn'nnn_ Cuts at: 0 635 641 651 Size: 635 6 10 Fragments arranged by size: 635 10 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 300 651 Size: 300 351 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 475 647 651 Size: 469 6 172 4 Fragments arranged by size: 469 172 6 4 HaeII r_GCGC'y Cuts at: 0 120 146 329 651 Size: 120 26 183 322 Fragments arranged by size: 322 183 120 26 KasI G'GCGC_C Cuts at: 0 325 651 Size: 325 326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 131 651 Size: 131 520 MspA1I CmG'CkG Cuts at: 0 595 651 Size: 595 56 NaeI GCC'GGC Cuts at: 0 213 228 651 Size: 213 15 423 Fragments arranged by size: 423 213 15 NarI GG'CG_CC Cuts at: 0 326 651 Size: 326 325 NgoMIV G'CCGG_C Cuts at: 0 211 226 651 Size: 211 15 425 Fragments arranged by size: 425 211 15 NspI r_CATG'y Cuts at: 0 261 651 Size: 261 390 PflMI CCAn_nnn'nTGG Cuts at: 0 447 651 Size: 447 204 PspOMI G'GGCC_C Cuts at: 0 597 651 Size: 597 54 PspXI vC'TCGA_Gb Cuts at: 0 238 651 Size: 238 413 SacII CC_GC'GG Cuts at: 0 596 651 Size: 596 55 SfoI GGC'GCC Cuts at: 0 327 651 Size: 327 324 SmaI CCC'GGG Cuts at: 0 122 602 651 Size: 122 480 49 Fragments arranged by size: 480 122 49 SmlI C'TyrA_G Cuts at: 0 48 238 651 Size: 48 190 413 Fragments arranged by size: 413 190 48 SrfI GCCC'GGGC Cuts at: 0 122 651 Size: 122 529 StyI C'CwwG_G Cuts at: 0 314 329 651 Size: 314 15 322 Fragments arranged by size: 322 314 15 XhoI C'TCGA_G Cuts at: 0 238 651 Size: 238 413 XmaI C'CCGG_G Cuts at: 0 120 600 651 Size: 120 480 51 Fragments arranged by size: 480 120 51 Enzymes that do cut: AccI AcuI AlwNI ApaI ApoI AvaI BanI BanII BbeI BcgI BglI Bme1580I BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsiEI BsmI Bsp1286I BspEI BsrBI BsrFI BssHII BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI HaeII KasI MmeI MspA1I NaeI NarI NgoMIV NspI PflMI PspOMI PspXI SacII SfoI SmaI SmlI SrfI StyI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaXI BsgI BsiHKAI BsiWI BsmBI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI