Restriction map for
pFN21AB8912
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AlwNI AocI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BpuAI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I HgaI HindIII HinfI HpaI
HphI KpnI Ksp632I KspI MaeI
MaeIII MboII MfeI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TfiI Tru9I Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 538 GAATTT [GA]^AATT[CT]
AcyI 566 GGCGCC G[GA]^CG[CT]C
AfaI 108 GTAC GT^AC
Alw44I 177 GTGCAC G^TGCAC
AlwI 681 GATCC GGATC(4/5)
ApaLI 177 GTGCAC G^TGCAC
ApoI 538 GAATTT [GA]^AATT[CT]
AviII 434 TGCGCA TGC^GCA
BanI 566 GGCGCC G^G[CT][GA]CC
BbeI 566 GGCGCC GGCGC^C
BbrPI 445 CACGTG CAC^GTG
BclI 20 TGATCA T^GATCA
BglII 620 AGATCT A^GATCT
BlpI 704 GCTCAGC GC^T[ACGT]AGC
BpmI 529 CTGGAG CTGGAG(16/14)
Bpu1102I 704 GCTCAGC GC^T[ACGT]AGC
BsaAI 445 CACGTG [CT]AC^GT[GA]
BsaBI 615 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 566 GGCGCC G[GA]^CG[CT]C
BseRI 304 CTCCTC GAGGAG(10/8)
BspMI 516 GCAGGT ACCTGC(4/8)
BsrBI 684 CCGCTC CCGCTC(-3/-3)
BstF5I 431 GGATG GGATG(2/0)
CelII 704 GCTCAGC GC^T[ACGT]AGC
CpoI 496 CGGACCG CG^G[AT]CCG
DdeI 705 CTCAG C^T[ACGT]AG
FbaI 20 TGATCA T^GATCA
FokI 431 GGATG GGATG(9/13)
FseI 724 GGCCGGCC GGCCGG^CC
FspI 434 TGCGCA TGC^GCA
Hin1I 566 GGCGCC G[GA]^CG[CT]C
HincII 37 GTCGAC GT[CT]^[GA]AC
HindII 37 GTCGAC GT[CT]^[GA]AC
KasI 566 GGCGCC G^GCGCC
MaeII 446 ACGT A^CGT
MamI 615 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 566 GGCGCC GG^CGCC
PmaCI 445 CACGTG CAC^GTG
PmlI 445 CACGTG CAC^GTG
PpuMI 206 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 108 GTAC GT^AC
RsrII 496 CGGACCG CG^G[AT]CCG
SalI 37 GTCGAC G^TCGAC
SfaNI 432 GATGC GCATC(5/9)
SfoI 566 GGCGCC GGC^GCC
SmaI 103 CCCGGG CCC^GGG
SspI 65 AATATT AAT^ATT
TaqI 38 TCGA T^CGA
TthHB8I 38 TCGA T^CGA
XmaCI 103 CCCGGG C^CCGGG
XmaI 103 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 37 GTCGAC GT^[AC][GT]AC
421 GTCTAC
AluI 139 AGCT AG^CT
527 AGCT
AspHI 177 GTGCAC G[AT]GC[AT]^C
450 GAGCAC
AvaII 207 GGACC G^G[AT]CC
497 GGACC
BsgI 379 CTGCAC GTGCAG(16/14)
184 GTGCAG
BsiHKAI 177 GTGCAC G[AT]GC[AT]^C
450 GAGCAC
BssHII 168 GCGCGC G^CGCGC
746 GCGCGC
BstYI 620 AGATCT [GA]^GATC[CT]
680 AGATCC
MflI 620 AGATCT [GA]^GATC[CT]
680 AGATCC
NlaIII 56 CATG CATG^
642 CATG
SgrAI 114 CGCCGGCG C[GA]^CCGG[CT]G
471 CGCCGGCG
Tsp509I 466 AATT ^AATT
539 AATT
XhoII 620 AGATCT [GA]^GATC[CT]
680 AGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI Aor51HI ApaI AvaI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
EaeI Eco47III EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MboI MnlI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIV Sau3AI
Sau96I ScrFI TseI