(Circular) (Six-base) MAPSORT of: pFN21AB8912.seq Check: 5717 from: 1 to: 5458 pFN21AB8912 5458 bp With 182 enzymes: SgfI * March 28, 2012 13:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3268 278 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2043 2427 2801 1937 Size: 106 384 374 4594 Fragments arranged by size: 4594 384 374 106 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3521 4905 5173 1632 Size: 1889 1384 268 1917 Fragments arranged by size: 1917 1889 1384 268 AfeI AGC'GCT Cuts at: 2195 2330 2495 2195 Size: 135 165 5158 Fragments arranged by size: 5158 165 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 4357 5334 1203 Size: 3154 977 1327 Fragments arranged by size: 3154 1327 977 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 4187 4187 Size: 5458 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5458 AlwNI CAG_nnn'CTG Cuts at: 3201 4773 3201 Size: 1572 3886 ApaI G_GGCC'C Cuts at: 2223 2308 2724 2223 Size: 85 416 4957 Fragments arranged by size: 4957 416 85 ApaLI G'TGCA_C Cuts at: 2182 3515 4671 2182 Size: 1333 1156 2969 Fragments arranged by size: 2969 1333 1156 ApoI r'AATT_y Cuts at: 1493 2543 2767 2993 3145 5254 1493 Size: 1050 224 226 152 2109 1697 Fragments arranged by size: 2109 1697 1050 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2277 2692 2783 1870 Size: 73 165 169 415 91 4545 Fragments arranged by size: 4545 415 169 165 91 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2571 2779 4234 618 Size: 325 217 627 784 208 1455 1842 Fragments arranged by size: 1842 1455 784 627 325 217 208 BanII G_rGCy'C Cuts at: 1391 2223 2284 2308 2322 2421 2490 2724 2777 Size: 832 61 24 14 99 69 234 53 Cuts at: 2777 1391 Size: 4072 Fragments arranged by size: 4072 832 234 99 69 61 53 24 14 BbeI G_GCGC'C Cuts at: 2575 2575 Size: 5458 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 2025 1548 Size: 477 4981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4069 136 Size: 107 122 71 631 605 2397 1525 Fragments arranged by size: 2397 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2625 5453 2625 Size: 2828 2630 BlpI GC'TnA_GC Cuts at: 2710 2875 2710 Size: 165 5293 Bme1580I G_kGCm'C Cuts at: 1790 2186 2223 2308 2724 3519 4675 1790 Size: 396 37 85 416 795 1156 2573 Fragments arranged by size: 2573 1156 795 416 396 85 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5458 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 4118 1239 Size: 1316 1563 2579 Fragments arranged by size: 2579 1563 1316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4448 4746 3583 Size: 865 298 4295 Fragments arranged by size: 4295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4121 882 Size: 617 2622 2219 Fragments arranged by size: 2622 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2452 3249 493 Size: 711 1248 797 2702 Fragments arranged by size: 2702 1248 797 711 BsaBI GATnn'nnATC Cuts at: 2624 5452 2624 Size: 2828 2630 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2572 3265 3647 4282 Size: 53 83 186 936 1039 693 382 635 Cuts at: 4282 275 Size: 1451 Fragments arranged by size: 1451 1039 936 693 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3891 4289 4563 4710 5121 3891 Size: 398 274 147 411 4228 Fragments arranged by size: 4228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5233 Fragments arranged by size: 5233 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 1186 Size: 1114 4344 BseYI C'CCAG_C Cuts at: 2093 2390 4661 5157 2093 Size: 297 2271 496 2394 Fragments arranged by size: 2394 2271 496 297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 2369 1163 Size: 573 474 159 4252 Fragments arranged by size: 4252 573 474 159 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3669 4697 1540 Size: 275 186 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 2459 3519 3604 4675 2186 Size: 273 1060 85 1071 2969 Fragments arranged by size: 2969 1071 1060 273 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2223 2284 2308 2322 2421 2459 Size: 399 396 37 61 24 14 99 38 Cuts at: 2459 2490 2724 2777 3519 3604 4675 1391 Size: 31 234 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 234 99 85 61 53 38 37 31 24 14 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2512 2813 844 Size: 1668 301 3489 Fragments arranged by size: 3489 1668 301 BsrBI CCG'CTC Cuts at: 1394 1823 2691 3345 1394 Size: 429 868 654 3507 Fragments arranged by size: 3507 868 654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 4127 65 Size: 1183 2705 174 1396 Fragments arranged by size: 2705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 2477 2640 2730 4102 4289 1700 Size: 110 310 357 163 90 1372 187 2869 Fragments arranged by size: 2869 1372 357 310 187 163 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5458 BssHII G'CGCG_C Cuts at: 1925 2173 2751 1925 Size: 248 578 4632 Fragments arranged by size: 4632 578 248 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5458 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5458 BstYI r'GATC_y Cuts at: 1972 2625 2685 2788 3155 3540 3557 5009 5095 Size: 653 60 103 367 385 17 1452 86 Cuts at: 5095 5107 5453 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 653 385 367 346 103 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3967 Fragments arranged by size: 3967 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4719 Fragments arranged by size: 4719 683 56 BtsI GCAGTG_nn' Cuts at: 2974 3768 3788 2974 Size: 794 20 4644 Fragments arranged by size: 4644 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2323 2728 Size: 54 1425 50 275 18 295 198 405 Cuts at: 2728 2905 3794 5423 8 Size: 177 889 1629 43 Fragments arranged by size: 1629 1425 889 405 295 275 198 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2905 1537 Size: 275 1093 4090 Fragments arranged by size: 4090 1093 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3388 1353 Size: 2035 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2223 4045 4419 4565 387 Size: 1064 138 634 1822 374 146 1280 Fragments arranged by size: 1822 1280 1064 634 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2555 3521 4118 4905 5173 1239 Size: 393 923 966 597 787 268 1524 Fragments arranged by size: 1524 966 923 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2212 2220 2304 2305 2720 2721 2744 2212 Size: 8 84 1 415 1 23 4926 Fragments arranged by size: 4926 415 84 23 8 1 1 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FseI GG_CCGG'CC Cuts at: 2734 2734 Size: 5458 FspI TGC'GCA Cuts at: 2441 3964 2441 Size: 1523 3935 HaeII r_GCGC'y Cuts at: 1678 2197 2332 2497 2575 2617 4605 1678 Size: 519 135 165 78 42 1988 2531 Fragments arranged by size: 2531 1988 519 165 135 78 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373 Size: 32 135 32 2527 32 42 32 2626 Fragments arranged by size: 2626 2527 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2044 2802 3048 669 Size: 988 281 106 758 246 3079 Fragments arranged by size: 3079 988 758 281 246 106 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KasI G'GCGC_C Cuts at: 2571 2571 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4572 4756 1305 Size: 3267 184 2007 Fragments arranged by size: 3267 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3416 3775 3934 518 Size: 648 843 1407 359 159 2042 Fragments arranged by size: 2042 1407 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2874 3551 4699 4944 1606 Size: 1268 677 1148 245 2120 Fragments arranged by size: 2120 1268 1148 677 245 NaeI GCC'GGC Cuts at: 2122 2479 2732 2122 Size: 357 253 4848 Fragments arranged by size: 4848 357 253 NarI GG'CG_CC Cuts at: 2572 2572 Size: 5458 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3967 Fragments arranged by size: 3967 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5458 NgoMIV G'CCGG_C Cuts at: 2120 2477 2730 2120 Size: 357 253 4848 Fragments arranged by size: 4848 357 253 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2816 4361 1132 Size: 97 1587 1545 2229 Fragments arranged by size: 2229 1587 1545 97 PasI CC'CwG_GG Cuts at: 1348 2216 1348 Size: 868 4590 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5458 PfoI T'CCnGG_A Cuts at: 2313 2313 Size: 5458 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PmlI CAC'GTG Cuts at: 2452 2452 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3527 3559 5040 5072 3527 Size: 32 1481 32 3913 Fragments arranged by size: 3913 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2212 Size: 5458 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5458 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PspOMI G'GGCC_C Cuts at: 2219 2304 2720 2219 Size: 85 416 4957 Fragments arranged by size: 4957 416 85 PstI C_TGCA'G Cuts at: 830 2810 830 Size: 1980 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 RsrII CG'GwC_CG Cuts at: 2502 2502 Size: 5458 SalI G'TCGA_C Cuts at: 1936 2042 2800 1936 Size: 106 758 4594 Fragments arranged by size: 4594 758 106 SanDI GG'GwC_CC Cuts at: 2212 2212 Size: 5458 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SfcI C'TryA_G Cuts at: 826 1045 2806 3941 4622 4813 826 Size: 219 1761 1135 681 191 1471 Fragments arranged by size: 1761 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2573 2573 Size: 5458 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 2120 2477 1700 Size: 420 357 4681 Fragments arranged by size: 4681 420 357 SmaI CCC'GGG Cuts at: 1872 2110 2785 1872 Size: 238 675 4545 Fragments arranged by size: 4545 675 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 3562 4463 4725 820 Size: 197 926 1619 901 262 1553 Fragments arranged by size: 1619 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2816 1132 Size: 1684 3774 SspI AAT'ATT Cuts at: 5 52 2072 3382 5 Size: 47 2020 1310 2081 Fragments arranged by size: 2081 2020 1310 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5458 StyI C'CwwG_G Cuts at: 513 1065 2004 2897 513 Size: 552 939 893 3074 Fragments arranged by size: 3074 939 893 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3485 3502 3655 3840 4285 325 Size: 1255 1905 17 153 185 445 1498 Fragments arranged by size: 1905 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3704 5416 96 Size: 274 80 33 51 1443 1727 1712 138 Fragments arranged by size: 1727 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5418 5450 5418 Size: 32 5426 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2108 2783 1870 Size: 238 675 4545 Fragments arranged by size: 4545 675 238 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3266 276 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI PvuII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI