################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvrII BalI BamHI BbrPI BbvCI BciVI BclI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsgI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MflI MluI MluNI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 877 GACGTC GACGT^C AccI 117 GTCGAC GT^[AC][GT]AC AccIII 955 TCCGGA T^CCGGA AflII 765 CTTAAG C^TTAAG AlwI 379 GGATC GGATC(4/5) AlwNI 460 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 123 ATTAAT AT^TAAT AsnI 123 ATTAAT AT^TAAT Asp700I 477 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 437 TGCGCA TGC^GCA BcgI 808 GCAGGCGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 971 CTAG C^TAG BfrI 765 CTTAAG C^TTAAG BsaI 192 GAGACC GGTCTC(1/5) BsaWI 955 TCCGGA [AT]^CCGG[AT] BseAI 955 TCCGGA T^CCGGA BsmBI 335 CGTCTC CGTCTC(1/5) BspEI 955 TCCGGA T^CCGGA BsrDI 866 CATTGC GCAATG(2/0) DdeI 798 CTTAG C^T[ACGT]AG DraII 760 GGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 991 GACAGCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 683 CCGCGG C^C[GA][CT]GG EarI 1015 GAAGAG CTCTTC(1/4) EcoO109I 760 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 481 GAATTC G^AATTC EcoT14I 286 CCTTGG C^C[AT][AT]GG FspI 437 TGCGCA TGC^GCA HincII 117 GTCGAC GT[CT]^[GA]AC HindII 117 GTCGAC GT[CT]^[GA]AC Ksp632I 1015 GAAGAG CTCTTC(1/4) KspI 683 CCGCGG CCGC^GG MaeI 971 CTAG C^TAG MaeIII 737 GTCAC ^GT[ACGT]AC MroI 955 TCCGGA T^CCGGA NlaIII 585 CATG CATG^ PshAI 937 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 123 ATTAAT AT^TAAT PvuII 460 CAGCTG CAG^CTG SacII 683 CCGCGG CCGC^GG SalI 117 GTCGAC G^TCGAC SapI 1015 GAAGAGC GCTCTTC(1/4) SmlI 765 CTTAAG C^T[CT][GA]AG StuI 960 AGGCCT AGG^CCT StyI 286 CCTTGG C^C[AT][AT]GG TfiI 898 GATTC G^A[AT]TC Tsp45I 737 GTCAC ^GT[GC]AC XmnI 477 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 498 GTAC GT^AC 1037 GTAC BanII 647 GAGCCC G[GA]GC[CT]^C 856 GAGCCC BbeI 175 GGCGCC GGCGC^C 890 GGCGCC BbsI 874 GAAGAC GAAGAC(2/6) 880 GTCTTC BmyI 647 GAGCCC G[AGT]GC[ACT]^C 856 GAGCCC BpuAI 874 GAAGAC GAAGAC(2/6) 880 GTCTTC BsiEI 347 CGGCCG CG[GA][CT]^CG 932 CGGCCG Bsp1286I 647 GAGCCC G[AGT]GC[ACT]^C 856 GAGCCC BspMI 1055 ACCTGC ACCTGC(4/8) 555 GCAGGT BsrI 471 ACTGG ACTGG(1/-1) 1034 CCAGT BssHII 427 GCGCGC G^CGCGC 830 GCGCGC BstF5I 290 GGATG GGATG(2/0) 624 GGATG DpnII 380 GATC ^GATC 525 GATC EagI 347 CGGCCG C^GGCCG 932 CGGCCG EclXI 347 CGGCCG C^GGCCG 932 CGGCCG Eco52I 347 CGGCCG C^GGCCG 932 CGGCCG FokI 290 GGATG GGATG(9/13) 624 GGATG HaeII 175 GGCGCC [GA]GCGC^[CT] 890 GGCGCC KasI 175 GGCGCC G^GCGCC 890 GGCGCC MaeII 334 ACGT A^CGT 878 ACGT MboI 380 GATC ^GATC 525 GATC NaeI 506 GCCGGC GCC^GGC 844 GCCGGC NarI 175 GGCGCC GG^CGCC 890 GGCGCC NdeII 380 GATC ^GATC 525 GATC NgoMIV 506 GCCGGC G^CCGGC 844 GCCGGC PstI 386 CTGCAG CTGCA^G 1057 CTGCAG RsaI 498 GTAC GT^AC 1037 GTAC Sau3AI 380 GATC ^GATC 525 GATC SfoI 175 GGCGCC GGC^GCC 890 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AcsI AcyI AluI ApoI AvaI AvaII BanI BbvI BcnI BglI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I EaeI EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PleI Sau96I ScrFI SfcI SmaI TaqI Tru9I TseI Tsp509I TthHB8I XmaCI XmaI