(Circular) (Six-base) MAPSORT of: pFN21AB8966.seq Check: 321 from: 1 to: 5773 pFN21AB8966 5773 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2886 3583 278 Size: 53 83 186 936 1350 697 2468 Fragments arranged by size: 2468 1350 936 697 186 83 53 AccI GT'mk_AC Cuts at: 1937 2123 3116 1937 Size: 186 993 4594 Fragments arranged by size: 4594 993 186 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 3836 5220 5488 1632 Size: 545 1659 1384 268 1917 Fragments arranged by size: 1917 1659 1384 545 268 AflII C'TTAA_G Cuts at: 820 1017 2770 820 Size: 197 1753 3823 Fragments arranged by size: 3823 1753 197 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 2470 3516 5088 2470 Size: 1046 1572 3155 Fragments arranged by size: 3155 1572 1046 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 2008 2015 2486 3082 3308 3460 5569 1493 Size: 515 7 471 596 226 152 2109 1697 Fragments arranged by size: 2109 1697 596 515 471 226 152 7 AseI AT'TA_AT Cuts at: 160 1032 2129 4327 160 Size: 872 1097 2198 1606 Fragments arranged by size: 2198 1606 1097 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 2282 2647 2698 2864 2890 3098 1870 Size: 73 339 365 51 166 26 208 4545 Fragments arranged by size: 4545 365 339 208 166 73 51 26 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 2758 2895 3094 4549 Size: 325 217 627 393 578 137 199 1455 Cuts at: 4549 618 Size: 1842 Fragments arranged by size: 1842 1455 627 578 393 325 217 199 137 BanII G_rGCy'C Cuts at: 1391 2656 2865 3092 1391 Size: 1265 209 227 4072 Fragments arranged by size: 4072 1265 227 209 BbeI G_GCGC'C Cuts at: 2184 2899 2184 Size: 715 5058 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2878 2886 928 Size: 971 979 8 3815 Fragments arranged by size: 3815 979 971 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2802 2836 3964 3998 1903 Size: 34 865 34 1128 34 3678 Fragments arranged by size: 3678 1128 865 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 1548 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2276 2369 2703 Size: 107 122 71 631 605 604 93 334 Cuts at: 2703 2720 4384 136 Size: 17 1664 1525 Fragments arranged by size: 1664 1525 631 605 604 334 122 107 93 71 17 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 3834 4990 1790 Size: 2044 1156 2573 Fragments arranged by size: 2573 2044 1156 BmgBI CAC'GTC Cuts at: 1318 2340 1318 Size: 1022 4751 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3504 4462 442 Size: 416 53 83 399 24 2087 958 1753 Fragments arranged by size: 2087 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2498 2678 2879 2936 4433 1239 Size: 917 342 180 201 57 1497 2579 Fragments arranged by size: 2579 1497 917 342 201 180 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2191 4436 882 Size: 617 692 2245 2219 Fragments arranged by size: 2245 2219 692 617 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 5767 5767 Size: 5773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 2883 2896 3580 Size: 53 83 186 936 648 702 13 684 Cuts at: 3580 3962 4597 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 702 684 648 635 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 2960 4206 4604 4878 5025 5436 2960 Size: 1246 398 274 147 411 3297 Fragments arranged by size: 3297 1246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 1178 Size: 30 165 30 615 30 4903 Fragments arranged by size: 4903 615 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2130 2133 1186 Size: 941 3 3 4826 Fragments arranged by size: 4826 941 3 3 BseYI C'CCAG_C Cuts at: 2165 2324 4976 5472 2165 Size: 159 2652 496 2466 Fragments arranged by size: 2652 2466 496 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5200 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2355 2940 3223 3984 5012 1540 Size: 275 186 354 585 283 761 1028 2301 Fragments arranged by size: 2301 1028 761 585 354 283 275 186 BsiHKAI G_wGCw'C Cuts at: 3834 3919 4990 3834 Size: 85 1071 4617 Fragments arranged by size: 4617 1071 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 BsmBI CGTCTCn'nnnn_ Cuts at: 2346 2346 Size: 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2656 2865 3092 3834 3919 4990 1391 Size: 399 866 209 227 742 85 1071 2174 Fragments arranged by size: 2174 1071 866 742 399 227 209 85 BspEI T'CCGG_A Cuts at: 2960 5436 2960 Size: 2476 3297 BspHI T'CATG_A Cuts at: 3662 5392 3662 Size: 1730 4043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2551 3069 3128 844 Size: 1707 518 59 3489 Fragments arranged by size: 3489 1707 518 59 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2870 4268 4442 65 Size: 1183 1622 1398 174 1396 Fragments arranged by size: 1622 1398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2511 2583 2849 4417 4604 1700 Size: 110 701 72 266 1568 187 2869 Fragments arranged by size: 2869 1568 701 266 187 110 72 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 2432 2835 1925 Size: 507 403 4863 Fragments arranged by size: 4863 507 403 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstYI r'GATC_y Cuts at: 1972 3103 3470 3855 3872 5324 5410 5422 5768 Size: 1131 367 385 17 1452 86 12 346 Cuts at: 5768 1972 Size: 1977 Fragments arranged by size: 1977 1452 1131 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2688 513 Size: 552 1623 3598 Fragments arranged by size: 3598 1623 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 3289 4083 4103 3289 Size: 794 20 4959 Fragments arranged by size: 4959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3002 4780 809 Size: 507 1686 1778 1802 Fragments arranged by size: 1802 1778 1686 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2352 2513 2691 Size: 54 1425 50 275 18 522 161 178 Cuts at: 2691 2937 3220 4109 5738 8 Size: 246 283 889 1629 43 Fragments arranged by size: 1629 1425 889 522 283 275 246 178 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2352 2937 3220 1537 Size: 275 540 585 283 4090 Fragments arranged by size: 4090 585 540 283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3015 3703 1353 Size: 1662 688 3423 Fragments arranged by size: 3423 1662 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2302 2323 2350 2701 4360 4734 Size: 1064 138 713 21 27 351 1659 374 Cuts at: 4734 4880 387 Size: 146 1280 Fragments arranged by size: 1659 1280 1064 713 374 351 146 138 27 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2177 2498 2678 2879 2936 3836 Size: 393 524 21 321 180 201 57 900 Cuts at: 3836 4433 5220 5488 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 900 787 597 524 393 321 268 201 180 57 21 EcoO109I rG'GnC_Cy Cuts at: 2766 2766 Size: 5773 EcoRI G'AATT_C Cuts at: 2486 3082 3460 2486 Size: 596 378 4799 Fragments arranged by size: 4799 596 378 FspI TGC'GCA Cuts at: 2444 4279 2444 Size: 1835 3938 HaeII r_GCGC'y Cuts at: 1678 2184 2899 4920 1678 Size: 506 715 2021 2531 Fragments arranged by size: 2531 2021 715 506 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2873 2905 4414 Size: 32 135 32 722 32 547 32 1509 Cuts at: 4414 4446 4488 4520 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1509 722 547 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2124 3117 3363 669 Size: 988 281 186 993 246 3079 Fragments arranged by size: 3079 993 988 281 246 186 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KasI G'GCGC_C Cuts at: 2180 2895 2180 Size: 715 5058 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2362 4887 5071 1305 Size: 1057 2525 184 2007 Fragments arranged by size: 2525 2007 1057 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3951 Fragments arranged by size: 3951 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3731 4090 4249 518 Size: 648 2565 359 159 2042 Fragments arranged by size: 2565 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2267 2467 2690 3189 3866 5014 5259 Size: 478 183 200 223 499 677 1148 245 Cuts at: 5259 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 499 478 245 223 200 183 NaeI GCC'GGC Cuts at: 2513 2851 2513 Size: 338 5435 NarI GG'CG_CC Cuts at: 2181 2896 2181 Size: 715 5058 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5221 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NgoMIV G'CCGG_C Cuts at: 2511 2849 2511 Size: 338 5435 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2946 1613 Size: 1333 4440 PsiI TTA'TAA Cuts at: 2039 3343 2039 Size: 1304 4469 PstI C_TGCA'G Cuts at: 830 2395 3066 3125 830 Size: 1565 671 59 3478 Fragments arranged by size: 3478 1565 671 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 2467 2467 Size: 5773 SacII CC_GC'GG Cuts at: 2691 2691 Size: 5773 SalI G'TCGA_C Cuts at: 1936 2122 3115 1936 Size: 186 993 4594 Fragments arranged by size: 4594 993 186 SapI GCTCTTCn'nnn_ Cuts at: 3015 3015 Size: 5773 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2391 2981 3062 3121 4256 4937 5128 Size: 219 1346 590 81 59 1135 681 191 Cuts at: 5128 826 Size: 1471 Fragments arranged by size: 1471 1346 1135 681 590 219 191 81 59 SfoI GGC'GCC Cuts at: 2182 2897 2182 Size: 715 5058 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 2649 2700 2866 3100 1872 Size: 777 51 166 234 4545 Fragments arranged by size: 4545 777 234 166 51 SmlI C'TyrA_G Cuts at: 820 1017 1943 2770 3877 4778 5040 820 Size: 197 926 827 1107 901 262 1553 Fragments arranged by size: 1553 1107 926 901 827 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SrfI GCCC'GGGC Cuts at: 2700 2866 2700 Size: 166 5607 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 2967 1517 Size: 1450 4323 StyI C'CwwG_G Cuts at: 513 1065 2291 3212 513 Size: 552 1226 921 3074 Fragments arranged by size: 3074 1226 921 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 3817 3970 4155 4600 325 Size: 1255 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4019 5731 96 Size: 274 80 33 51 1443 2042 1712 138 Fragments arranged by size: 2042 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 2647 2698 2864 3098 1870 Size: 777 51 166 234 4545 Fragments arranged by size: 4545 777 234 166 51 XmnI GAAnn'nnTTC Cuts at: 2486 3902 2486 Size: 1416 4357 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2884 3581 276 Size: 53 83 186 936 1350 697 2468 Fragments arranged by size: 2468 1350 936 697 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SwaI XcmI