(Linear) (Six-base) MAPSORT of: KIBB8966.seq Check: 1086 from: 1 to: 1068 KIBB8966.orf With 182 enzymes: SgfI * December 27, 2011 16:30 .. AatII G_ACGT'C Cuts at: 0 881 1068 Size: 881 187 AccI GT'mk_AC Cuts at: 0 118 1068 Size: 118 950 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 1068 Size: 172 896 AflII C'TTAA_G Cuts at: 0 765 1068 Size: 765 303 AlwNI CAG_nnn'CTG Cuts at: 0 465 1068 Size: 465 603 ApoI r'AATT_y Cuts at: 0 3 10 481 1068 Size: 3 7 471 587 Fragments arranged by size: 587 471 7 3 AseI AT'TA_AT Cuts at: 0 124 1068 Size: 124 944 AvaI C'yCGr_G Cuts at: 0 277 642 693 859 885 1068 Size: 277 365 51 166 26 183 Fragments arranged by size: 365 277 183 166 51 26 BanI G'GyrC_C Cuts at: 0 175 753 890 1068 Size: 175 578 137 178 Fragments arranged by size: 578 178 175 137 BanII G_rGCy'C Cuts at: 0 651 860 1068 Size: 651 209 208 Fragments arranged by size: 651 209 208 BbeI G_GCGC'C Cuts at: 0 179 894 1068 Size: 179 715 174 Fragments arranged by size: 715 179 174 BbsI GAAGACnn'nnnn_ Cuts at: 0 873 881 1068 Size: 873 8 187 Fragments arranged by size: 873 187 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 797 831 1068 Size: 797 34 237 Fragments arranged by size: 797 237 34 BglI GCCn_nnn'nGGC Cuts at: 0 271 364 698 715 1068 Size: 271 93 334 17 353 Fragments arranged by size: 353 334 271 93 17 BmgBI CAC'GTC Cuts at: 0 335 1068 Size: 335 733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 493 673 874 931 1068 Size: 151 342 180 201 57 137 Fragments arranged by size: 342 201 180 151 137 57 BsaI GGTCTCn'nnnn_ Cuts at: 0 186 1068 Size: 186 882 BsaHI Gr'CG_yC Cuts at: 0 176 878 891 1068 Size: 176 702 13 177 Fragments arranged by size: 702 177 176 13 BsaWI w'CCGG_w Cuts at: 0 955 1068 Size: 955 113 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 13 43 1068 Size: 13 30 1025 Fragments arranged by size: 1025 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 122 125 128 1068 Size: 122 3 3 940 Fragments arranged by size: 940 122 3 3 BseYI C'CCAG_C Cuts at: 0 160 319 1068 Size: 160 159 749 Fragments arranged by size: 749 160 159 BsiEI CG_ry'CG Cuts at: 0 350 935 1068 Size: 350 585 133 Fragments arranged by size: 585 350 133 BsmBI CGTCTCn'nnnn_ Cuts at: 0 341 1068 Size: 341 727 Bsp1286I G_dGCh'C Cuts at: 0 651 860 1068 Size: 651 209 208 Fragments arranged by size: 651 209 208 BspEI T'CCGG_A Cuts at: 0 955 1068 Size: 955 113 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 546 1064 1068 Size: 546 518 4 Fragments arranged by size: 546 518 4 BsrDI GCAATG_nn' Cuts at: 0 865 1068 Size: 865 203 BsrFI r'CCGG_y Cuts at: 0 506 578 844 1068 Size: 506 72 266 224 Fragments arranged by size: 506 266 224 72 BssHII G'CGCG_C Cuts at: 0 427 830 1068 Size: 427 403 238 Fragments arranged by size: 427 403 238 BtgI C'CryG_G Cuts at: 0 683 1068 Size: 683 385 DrdI GACnn_nn'nnGTC Cuts at: 0 997 1068 Size: 997 71 EaeI y'GGCC_r Cuts at: 0 347 508 686 932 1068 Size: 347 161 178 246 136 Fragments arranged by size: 347 246 178 161 136 EagI C'GGCC_G Cuts at: 0 347 932 1068 Size: 347 585 136 Fragments arranged by size: 585 347 136 EarI CTCTTCn'nnn_ Cuts at: 0 1010 1068 Size: 1010 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 297 318 345 696 1068 Size: 297 21 27 351 372 Fragments arranged by size: 372 351 297 27 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 172 493 673 874 931 1068 Size: 151 21 321 180 201 57 137 Fragments arranged by size: 321 201 180 151 137 57 21 EcoO109I rG'GnC_Cy Cuts at: 0 761 1068 Size: 761 307 EcoRI G'AATT_C Cuts at: 0 481 1068 Size: 481 587 FspI TGC'GCA Cuts at: 0 439 1068 Size: 439 629 HaeII r_GCGC'y Cuts at: 0 179 894 1068 Size: 179 715 174 Fragments arranged by size: 715 179 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 868 900 1068 Size: 289 32 547 32 168 Fragments arranged by size: 547 289 168 32 32 HincII GTy'rAC Cuts at: 0 119 1068 Size: 119 949 KasI G'GCGC_C Cuts at: 0 175 890 1068 Size: 175 715 178 Fragments arranged by size: 715 178 175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 357 1068 Size: 357 711 MspA1I CmG'CkG Cuts at: 0 79 262 462 685 1068 Size: 79 183 200 223 383 Fragments arranged by size: 383 223 200 183 79 NaeI GCC'GGC Cuts at: 0 508 846 1068 Size: 508 338 222 Fragments arranged by size: 508 338 222 NarI GG'CG_CC Cuts at: 0 176 891 1068 Size: 176 715 177 Fragments arranged by size: 715 177 176 NgoMIV G'CCGG_C Cuts at: 0 506 844 1068 Size: 506 338 224 Fragments arranged by size: 506 338 224 PshAI GACnn'nnGTC Cuts at: 0 941 1068 Size: 941 127 PsiI TTA'TAA Cuts at: 0 34 1068 Size: 34 1034 PstI C_TGCA'G Cuts at: 0 390 1061 1068 Size: 390 671 7 Fragments arranged by size: 671 390 7 PvuII CAG'CTG Cuts at: 0 462 1068 Size: 462 606 SacII CC_GC'GG Cuts at: 0 686 1068 Size: 686 382 SalI G'TCGA_C Cuts at: 0 117 1068 Size: 117 951 SapI GCTCTTCn'nnn_ Cuts at: 0 1010 1068 Size: 1010 58 SfcI C'TryA_G Cuts at: 0 386 976 1057 1068 Size: 386 590 81 11 Fragments arranged by size: 590 386 81 11 SfoI GGC'GCC Cuts at: 0 177 892 1068 Size: 177 715 176 Fragments arranged by size: 715 177 176 SmaI CCC'GGG Cuts at: 0 644 695 861 1068 Size: 644 51 166 207 Fragments arranged by size: 644 207 166 51 SmlI C'TyrA_G Cuts at: 0 765 1068 Size: 765 303 SrfI GCCC'GGGC Cuts at: 0 695 861 1068 Size: 695 166 207 Fragments arranged by size: 695 207 166 StuI AGG'CCT Cuts at: 0 962 1068 Size: 962 106 StyI C'CwwG_G Cuts at: 0 286 1068 Size: 286 782 XmaI C'CCGG_G Cuts at: 0 642 693 859 1068 Size: 642 51 166 209 Fragments arranged by size: 642 209 166 51 XmnI GAAnn'nnTTC Cuts at: 0 481 1068 Size: 481 587 ZraI GAC'GTC Cuts at: 0 879 1068 Size: 879 189 Enzymes that do cut: AatII AccI AcuI AflII AlwNI ApoI AseI AvaI BanI BanII BbeI BbsI BcgI BglI BmgBI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BtgI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII KasI MmeI MspA1I NaeI NarI NgoMIV PshAI PsiI PstI PvuII SacII SalI SapI SfcI SfoI SmaI SmlI SrfI StuI StyI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsgI BsiHKAI BsiWI BsmI BspHI BsrBI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI