################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DpnII DraI DraIII EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NarI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AflIII 625 ACGTGT A^C[GA][CT]GT AspHI 912 GTGCTC G[AT]GC[AT]^C BfaI 775 CTAG C^TAG BglI 302 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 226 GAGACC GGTCTC(1/5) BseRI 1045 GAGGAG GAGGAG(10/8) BsiHKAI 912 GTGCTC G[AT]GC[AT]^C BsmBI 697 GAGACG CGTCTC(1/5) BsrDI 1032 GCAATG GCAATG(2/0) BstF5I 237 GGATG GGATG(2/0) BstXI 812 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 727 GACTCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 636 CCATGG C^C[GA][CT]GG EaeI 26 TGGCCG [CT]^GGCC[GA] EarI 606 GAAGAG CTCTTC(1/4) FokI 237 GGATG GGATG(9/13) Ksp632I 606 GAAGAG CTCTTC(1/4) MaeI 775 CTAG C^TAG MboII 606 GAAGA GAAGA(8/7) MspA1I 927 CAGCTG C[AC]G^C[GT]G NcoI 636 CCATGG C^CATGG PvuII 927 CAGCTG CAG^CTG ScaI 884 AGTACT AGT^ACT SexAI 839 ACCTGGT A^CC[AT]GGT XcmI 755 CCAATGAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccII 15 CGCG CG^CG 360 CGCG AcyI 99 GACGCC G[GA]^CG[CT]C 571 GACGCC AlwNI 706 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 864 CAGCTTCTG AocI 231 CCTGAGG CC^T[ACGT]AGG 615 CCTCAGG ApaI 146 GGGCCC GGGCC^C 785 GGGCCC AvaI 395 CCCGGG C^[CT]CG[GA]G 917 CCCGGG AviII 243 TGCGCA TGC^GCA 662 TGCGCA BbrPI 586 CACGTG CAC^GTG 624 CACGTG BpmI 304 CTGGAG CTGGAG(16/14) 451 CTGGAG BsaHI 99 GACGCC G[GA]^CG[CT]C 571 GACGCC BsmFI 836 GGGAC GGGAC(10/14) 180 GTCCC BsoBI 395 CCCGGG C^[CT]CG[GA]G 917 CCCGGG Bsp1407I 845 TGTACA T^GTACA 1055 TGTACA BsrFI 458 GCCGGC [GA]^CCGG[CT] 872 GCCGGC BsrGI 845 TGTACA T^GTACA 1055 TGTACA BsrI 267 CCAGT ACTGG(1/-1) 746 CCAGT BstAPI 935 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1032 GCAATGTGTGC BstUI 15 CGCG CG^CG 360 CGCG Bsu36I 231 CCTGAGG CC^T[ACGT]AGG 615 CCTCAGG Cfr10I 458 GCCGGC [GA]^CCGG[CT] 872 GCCGGC Eco81I 231 CCTGAGG CC^T[ACGT]AGG 615 CCTCAGG FspI 243 TGCGCA TGC^GCA 662 TGCGCA HgaI 99 GACGC GACGC(5/10) 571 GACGC Hin1I 99 GACGCC G[GA]^CG[CT]C 571 GACGCC MaeIII 164 GTGAC ^GT[ACGT]AC 488 GTGAC MvnI 15 CGCG CG^CG 360 CGCG NaeI 458 GCCGGC GCC^GGC 872 GCCGGC NgoMIV 458 GCCGGC G^CCGGC 872 GCCGGC PflMI 384 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 826 CCAAGGTCTGG PmaCI 586 CACGTG CAC^GTG 624 CACGTG PmlI 586 CACGTG CAC^GTG 624 CACGTG PpuMI 551 AGGACCT [GA]G^G[AT]CC[CT] 836 GGGACCT PstI 402 CTGCAG CTGCA^G 930 CTGCAG SfaNI 238 GATGC GCATC(5/9) 593 GCATC SmaI 395 CCCGGG CCC^GGG 917 CCCGGG SspBI 845 TGTACA T^GTACA 1055 TGTACA Tsp45I 164 GTGAC ^GT[GC]AC 488 GTGAC Van91I 384 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 826 CCAAGGTCTGG XmaCI 395 CCCGGG C^CCGGG 917 CCCGGG XmaI 395 CCCGGG C^CCGGG 917 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI Aor51HI AvaII BanII BbvI BcnI BmyI BsaAI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfcI SmlI StuI StyI TseI TspRI