(Circular) (Six-base) MAPSORT of: pFN21AE1840.seq Check: 1784 from: 1 to: 5776 pFN21AE1840 5776 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2681 3074 2681 Size: 393 5383 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 2198 3839 5223 5491 1632 Size: 548 18 1641 1384 268 1917 Fragments arranged by size: 1917 1641 1384 548 268 18 AfeI AGC'GCT Cuts at: 2294 2413 2429 2294 Size: 119 16 5641 Fragments arranged by size: 5641 119 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2630 4675 5652 1203 Size: 1427 2045 977 1327 Fragments arranged by size: 2045 1427 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2716 2874 3519 5091 2716 Size: 158 645 1572 3401 Fragments arranged by size: 3401 1572 645 158 ApaI G_GGCC'C Cuts at: 2155 2794 2155 Size: 639 5137 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2400 2922 3101 1870 Size: 73 457 522 179 4545 Fragments arranged by size: 4545 522 457 179 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2155 2220 2794 3095 1391 Size: 649 115 65 574 301 4072 Fragments arranged by size: 4072 649 574 301 115 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3967 4001 1903 Size: 34 50 34 1946 34 3678 Fragments arranged by size: 3678 1946 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2313 4387 136 Size: 107 122 71 631 605 641 2074 1525 Fragments arranged by size: 2074 1525 641 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2126 2155 2569 2794 3837 4993 1790 Size: 336 29 414 225 1043 1156 2573 Fragments arranged by size: 2573 1156 1043 414 336 225 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 2477 4436 1239 Size: 1091 147 1959 2579 Fragments arranged by size: 2579 1959 1091 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2285 2315 2486 3901 4766 5064 2285 Size: 30 171 1415 865 298 2997 Fragments arranged by size: 2997 1415 865 298 171 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 4439 882 Size: 617 726 2214 2219 Fragments arranged by size: 2219 2214 726 617 BsaAI yAC'GTr Cuts at: 493 1204 2593 2631 3025 3567 493 Size: 711 1389 38 394 542 2702 Fragments arranged by size: 2702 1389 711 542 394 38 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2105 2577 3583 3965 Size: 53 83 186 936 572 472 1006 382 Cuts at: 3965 4600 275 Size: 635 1451 Fragments arranged by size: 1451 1006 936 635 572 472 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3044 3074 1178 Size: 30 165 30 1641 30 3880 Fragments arranged by size: 3880 1641 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3065 1186 Size: 1879 3897 BseYI C'CCAG_C Cuts at: 2414 2867 2882 4979 5475 2414 Size: 453 15 2097 496 2715 Fragments arranged by size: 2715 2097 496 453 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2416 3029 3065 1163 Size: 573 680 613 36 3874 Fragments arranged by size: 3874 680 613 573 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2921 3837 3922 4993 2921 Size: 916 85 1071 3704 Fragments arranged by size: 3704 1071 916 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2696 2696 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2126 2155 2220 2569 2794 2921 Size: 399 250 86 29 65 349 225 127 Cuts at: 2921 3095 3837 3922 4993 1391 Size: 174 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 250 225 174 127 86 85 65 29 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2568 2681 2897 3074 3131 844 Size: 1337 387 113 216 177 57 3489 Fragments arranged by size: 3489 1337 387 216 177 113 57 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3044 4271 4445 65 Size: 1183 1796 1227 174 1396 Fragments arranged by size: 1796 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2463 2877 4420 4607 1700 Size: 110 653 414 1543 187 2869 Fragments arranged by size: 2869 1543 653 414 187 110 BsrGI T'GTAC_A Cuts at: 96 2850 3060 96 Size: 2754 210 2812 Fragments arranged by size: 2812 2754 210 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 2946 3043 794 Size: 2152 97 3527 Fragments arranged by size: 3527 2152 97 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2824 2824 Size: 5776 BstYI r'GATC_y Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771 Size: 1134 367 385 17 1452 86 12 346 Cuts at: 5771 1972 Size: 1977 Fragments arranged by size: 1977 1452 1134 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2237 2621 2237 Size: 384 5392 BtgI C'CryG_G Cuts at: 513 1065 2641 513 Size: 552 1576 3648 Fragments arranged by size: 3648 1576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2171 2483 2998 3017 3292 4086 4106 2124 Size: 47 312 515 19 275 794 20 3794 Fragments arranged by size: 3794 794 515 312 275 47 20 19 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2738 4783 809 Size: 507 1422 2045 1802 Fragments arranged by size: 2045 1802 1422 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3223 4112 Size: 54 1425 50 275 18 201 1192 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1192 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2606 3706 1353 Size: 1253 1100 3423 Fragments arranged by size: 3423 1253 1100 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2198 2330 2477 3839 4436 5223 Size: 393 548 18 132 147 1362 597 787 Cuts at: 5223 5491 1239 Size: 268 1524 Fragments arranged by size: 1524 1362 787 597 548 393 268 147 132 18 EcoO109I rG'GnC_Cy Cuts at: 2151 2557 2791 2842 2151 Size: 406 234 51 5085 Fragments arranged by size: 5085 406 234 51 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 5776 FspI TGC'GCA Cuts at: 2250 2669 4282 2250 Size: 419 1613 3744 Fragments arranged by size: 3744 1613 419 FspAI rTGC'GCAy Cuts at: 2669 2669 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2054 2296 2415 2431 4923 1678 Size: 376 242 119 16 2492 2531 Fragments arranged by size: 2531 2492 376 242 119 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2934 3192 3869 5017 5262 1606 Size: 1328 258 677 1148 245 2120 Fragments arranged by size: 2120 1328 1148 677 258 245 NaeI GCC'GGC Cuts at: 2465 2879 2465 Size: 414 5362 NcoI C'CATG_G Cuts at: 513 1065 2641 513 Size: 552 1576 3648 Fragments arranged by size: 3648 1576 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NgoMIV G'CCGG_C Cuts at: 2463 2877 2463 Size: 414 5362 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2395 2837 1108 Size: 1287 442 4047 Fragments arranged by size: 4047 1287 442 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PmlI CAC'GTG Cuts at: 2593 2631 2593 Size: 38 5738 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2557 2842 2557 Size: 285 5491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2151 2790 2151 Size: 639 5137 PstI C_TGCA'G Cuts at: 830 2411 2939 3128 830 Size: 1581 528 189 3478 Fragments arranged by size: 3478 1581 528 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2934 2934 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2891 4024 5736 2891 Size: 1133 1712 2931 Fragments arranged by size: 2931 1712 1133 SexAI A'CCwGG_T Cuts at: 2844 2844 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2302 2407 2935 3124 4259 4940 5131 Size: 219 1257 105 528 189 1135 681 191 Cuts at: 5131 826 Size: 1471 Fragments arranged by size: 1471 1257 1135 681 528 219 191 189 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2402 2924 3103 1872 Size: 530 522 179 4545 Fragments arranged by size: 4545 530 522 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2330 2501 3880 4781 5043 Size: 197 926 321 66 171 1379 901 262 Cuts at: 5043 820 Size: 1553 Fragments arranged by size: 1553 1379 926 901 321 262 197 171 66 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SrfI GCCC'GGGC Cuts at: 2402 2402 Size: 5776 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2471 2768 2823 1517 Size: 954 297 55 4470 Fragments arranged by size: 4470 954 297 55 StyI C'CwwG_G Cuts at: 513 1065 2641 2769 2831 3215 513 Size: 552 1576 128 62 384 3074 Fragments arranged by size: 3074 1576 552 384 128 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2402 2881 2984 3803 3820 3973 4158 Size: 1255 822 479 103 819 17 153 185 Cuts at: 4158 4603 325 Size: 445 1498 Fragments arranged by size: 1498 1255 822 819 479 445 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 2850 2889 Size: 274 80 33 51 1443 528 345 39 Cuts at: 2889 3060 4022 5734 96 Size: 171 962 1712 138 Fragments arranged by size: 1712 1443 962 528 345 274 171 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 2767 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 2400 2922 3101 1870 Size: 530 522 179 4545 Fragments arranged by size: 4545 530 522 179 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SwaI