(Linear) (Six-base) MAPSORT of: KIEE1840.seq Check: 9324 from: 1 to: 1071 KIEE1840.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 676 1069 1071 Size: 676 393 2 Fragments arranged by size: 676 393 2 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 193 1071 Size: 175 18 878 Fragments arranged by size: 878 175 18 AfeI AGC'GCT Cuts at: 0 289 408 424 1071 Size: 289 119 16 647 Fragments arranged by size: 647 289 119 16 AflIII A'CryG_T Cuts at: 0 625 1071 Size: 625 446 AlwNI CAG_nnn'CTG Cuts at: 0 711 869 1071 Size: 711 158 202 Fragments arranged by size: 711 202 158 ApaI G_GGCC'C Cuts at: 0 150 789 1071 Size: 150 639 282 Fragments arranged by size: 639 282 150 AvaI C'yCGr_G Cuts at: 0 395 917 1071 Size: 395 522 154 Fragments arranged by size: 522 395 154 BanII G_rGCy'C Cuts at: 0 35 150 215 789 1071 Size: 35 115 65 574 282 Fragments arranged by size: 574 282 115 65 35 BglI GCCn_nnn'nGGC Cuts at: 0 308 1071 Size: 308 763 Bme1580I G_kGCm'C Cuts at: 0 121 150 564 789 1071 Size: 121 29 414 225 282 Fragments arranged by size: 414 282 225 121 29 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 472 1071 Size: 325 147 599 Fragments arranged by size: 599 325 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 310 481 1071 Size: 280 30 171 590 Fragments arranged by size: 590 280 171 30 BsaI GGTCTCn'nnnn_ Cuts at: 0 220 1071 Size: 220 851 BsaAI yAC'GTr Cuts at: 0 588 626 1020 1071 Size: 588 38 394 51 Fragments arranged by size: 588 394 51 38 BsaHI Gr'CG_yC Cuts at: 0 100 572 1071 Size: 100 472 499 Fragments arranged by size: 499 472 100 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1039 1069 1071 Size: 1039 30 2 Fragments arranged by size: 1039 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1060 1071 Size: 1060 11 BseYI C'CCAG_C Cuts at: 0 409 862 877 1071 Size: 409 453 15 194 Fragments arranged by size: 453 409 194 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 411 1024 1060 1071 Size: 411 613 36 11 Fragments arranged by size: 613 411 36 11 BsiHKAI G_wGCw'C Cuts at: 0 916 1071 Size: 916 155 BsmBI CGTCTCn'nnnn_ Cuts at: 0 691 1071 Size: 691 380 Bsp1286I G_dGCh'C Cuts at: 0 35 121 150 215 564 789 916 1071 Size: 35 86 29 65 349 225 127 155 Fragments arranged by size: 349 225 155 127 86 65 35 29 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 176 563 676 892 1069 1071 Size: 176 387 113 216 177 2 Fragments arranged by size: 387 216 177 176 113 2 BsrDI GCAATG_nn' Cuts at: 0 1039 1071 Size: 1039 32 BsrFI r'CCGG_y Cuts at: 0 458 872 1071 Size: 458 414 199 Fragments arranged by size: 458 414 199 BsrGI T'GTAC_A Cuts at: 0 845 1055 1071 Size: 845 210 16 Fragments arranged by size: 845 210 16 BstAPI GCAn_nnn'nTGC Cuts at: 0 941 1038 1071 Size: 941 97 33 Fragments arranged by size: 941 97 33 BstXI CCAn_nnnn'nTGG Cuts at: 0 819 1071 Size: 819 252 Bsu36I CC'TnA_GG Cuts at: 0 232 616 1071 Size: 232 384 455 Fragments arranged by size: 455 384 232 BtgI C'CryG_G Cuts at: 0 636 1071 Size: 636 435 BtsI GCAGTG_nn' Cuts at: 0 119 166 478 993 1012 1071 Size: 119 47 312 515 19 59 Fragments arranged by size: 515 312 119 59 47 19 DrdI GACnn_nn'nnGTC Cuts at: 0 733 1071 Size: 733 338 EaeI y'GGCC_r Cuts at: 0 26 1071 Size: 26 1045 EarI CTCTTCn'nnn_ Cuts at: 0 601 1071 Size: 601 470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 193 325 472 1071 Size: 175 18 132 147 599 Fragments arranged by size: 599 175 147 132 18 EcoO109I rG'GnC_Cy Cuts at: 0 146 552 786 837 1071 Size: 146 406 234 51 234 Fragments arranged by size: 406 234 234 146 51 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 351 1071 Size: 351 720 FspI TGC'GCA Cuts at: 0 245 664 1071 Size: 245 419 407 Fragments arranged by size: 419 407 245 FspAI rTGC'GCAy Cuts at: 0 664 1071 Size: 664 407 HaeII r_GCGC'y Cuts at: 0 49 291 410 426 1071 Size: 49 242 119 16 645 Fragments arranged by size: 645 242 119 49 16 MspA1I CmG'CkG Cuts at: 0 929 1071 Size: 929 142 NaeI GCC'GGC Cuts at: 0 460 874 1071 Size: 460 414 197 Fragments arranged by size: 460 414 197 NcoI C'CATG_G Cuts at: 0 636 1071 Size: 636 435 NgoMIV G'CCGG_C Cuts at: 0 458 872 1071 Size: 458 414 199 Fragments arranged by size: 458 414 199 PflMI CCAn_nnn'nTGG Cuts at: 0 390 832 1071 Size: 390 442 239 Fragments arranged by size: 442 390 239 PmlI CAC'GTG Cuts at: 0 588 626 1071 Size: 588 38 445 Fragments arranged by size: 588 445 38 PpuMI rG'GwC_Cy Cuts at: 0 552 837 1071 Size: 552 285 234 Fragments arranged by size: 552 285 234 PspOMI G'GGCC_C Cuts at: 0 146 785 1071 Size: 146 639 286 Fragments arranged by size: 639 286 146 PstI C_TGCA'G Cuts at: 0 406 934 1071 Size: 406 528 137 Fragments arranged by size: 528 406 137 PvuII CAG'CTG Cuts at: 0 929 1071 Size: 929 142 ScaI AGT'ACT Cuts at: 0 886 1071 Size: 886 185 SexAI A'CCwGG_T Cuts at: 0 839 1071 Size: 839 232 SfcI C'TryA_G Cuts at: 0 297 402 930 1071 Size: 297 105 528 141 Fragments arranged by size: 528 297 141 105 SmaI CCC'GGG Cuts at: 0 397 919 1071 Size: 397 522 152 Fragments arranged by size: 522 397 152 SmlI C'TyrA_G Cuts at: 0 259 325 496 1071 Size: 259 66 171 575 Fragments arranged by size: 575 259 171 66 SrfI GCCC'GGGC Cuts at: 0 397 1071 Size: 397 674 StuI AGG'CCT Cuts at: 0 466 763 818 1071 Size: 466 297 55 253 Fragments arranged by size: 466 297 253 55 StyI C'CwwG_G Cuts at: 0 636 764 826 1071 Size: 636 128 62 245 Fragments arranged by size: 636 245 128 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 397 876 979 1071 Size: 397 479 103 92 Fragments arranged by size: 479 397 103 92 TatI w'GTAC_w Cuts at: 0 500 845 884 1055 1071 Size: 500 345 39 171 16 Fragments arranged by size: 500 345 171 39 16 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 762 1071 Size: 762 309 XmaI C'CCGG_G Cuts at: 0 395 917 1071 Size: 395 522 154 Fragments arranged by size: 522 395 154 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI ApaI AvaI BanII BglI Bme1580I BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstXI Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I FalI FspI FspAI HaeII MspA1I NaeI NcoI NgoMIV PflMI PmlI PpuMI PspOMI PstI PvuII ScaI SexAI SfcI SmaI SmlI SrfI StuI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI