Restriction map for
pFN21AE6614
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbsI BbvCI BciVI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI HindIII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MslI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 300 GACGTC GACGT^C
AcyI 300 GACGTC G[GA]^CG[CT]C
Aor51HI 353 AGCGCT AGC^GCT
AspHI 513 GAGCAC G[AT]GC[AT]^C
AvaI 295 CCCGAG C^[CT]CG[GA]G
BalI 257 TGGCCA TGG^CCA
BamHI 494 GGATCC G^GATCC
BbrPI 460 CACGTG CAC^GTG
BcgI 297 CGAGACGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 456 TGATCA T^GATCA
BglI 339 GCCAAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 460 CACGTG [CT]AC^GT[GA]
BsaHI 300 GACGTC G[GA]^CG[CT]C
BsgI 53 CTGCAC GTGCAG(16/14)
BsiHKAI 513 GAGCAC G[AT]GC[AT]^C
BsmAI 298 GAGAC GTCTC(1/5)
BsmBI 298 GAGACG CGTCTC(1/5)
BsmI 87 GAATGC GAATGC(1/-1)
BsoBI 295 CCCGAG C^[CT]CG[GA]G
BsrBI 178 GAGCGG CCGCTC(-3/-3)
BsrFI 468 GCCGGC [GA]^CCGG[CT]
BstF5I 252 GGATG GGATG(2/0)
Cfr10I 468 GCCGGC [GA]^CCGG[CT]
DraII 241 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 599 CCATGG C^C[GA][CT]GG
EaeI 257 TGGCCA [CT]^GGCC[GA]
EarI 400 CTCTTC CTCTTC(1/4)
Eco47III 353 AGCGCT AGC^GCT
EcoO109I 241 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 456 TGATCA T^GATCA
FokI 252 GGATG GGATG(9/13)
Hin1I 300 GACGTC G[GA]^CG[CT]C
HincII 721 GTTGAC GT[CT]^[GA]AC
HindII 721 GTTGAC GT[CT]^[GA]AC
HphI 527 TCACC GGTGA(8/7)
Ksp632I 400 CTCTTC CTCTTC(1/4)
MboII 401 TCTTC GAAGA(8/7)
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
NaeI 468 GCCGGC GCC^GGC
NcoI 599 CCATGG C^CATGG
NgoMIV 468 GCCGGC G^CCGGC
NlaIII 600 CATG CATG^
PflMI 249 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 460 CACGTG CAC^GTG
PmlI 460 CACGTG CAC^GTG
PpuMI 241 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 633 CAGCTG CAG^CTG
SbfI 693 CCTGCAGG CCTGCA^GG
SexAI 716 ACCAGGT A^CC[AT]GGT
SfaNI 157 GCATC GCATC(5/9)
Sse8387I 693 CCTGCAGG CCTGCA^GG
Tsp45I 224 GTCAC ^GT[GC]AC
Van91I 249 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 180 GCGG CCGC(-3/-1)
484 GCGG
AfaI 75 GTAC GT^AC
714 GTAC
AvaII 242 GGACC G^G[AT]CC
382 GGTCC
BanII 628 GGGCTC G[GA]GC[CT]^C
670 GGGCTC
BlpI 546 GCTGAGC GC^T[ACGT]AGC
630 GCTCAGC
Bpu1102I 546 GCTGAGC GC^T[ACGT]AGC
630 GCTCAGC
BstAPI 55 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
696 GCAGGAGCTGC
BstYI 494 GGATCC [GA]^GATC[CT]
613 AGATCC
CelII 546 GCTGAGC GC^T[ACGT]AGC
630 GCTCAGC
CfoI 354 GCGC GCG^C
522 GCGC
EcoT14I 385 CCAAGG C^C[AT][AT]GG
599 CCATGG
HaeII 353 AGCGCT [GA]GCGC^[CT]
521 GGCGCT
HhaI 354 GCGC GCG^C
522 GCGC
HinP1I 354 GCGC G^CGC
522 GCGC
MaeII 301 ACGT A^CGT
461 ACGT
MaeIII 99 GTTAC ^GT[ACGT]AC
224 GTCAC
MflI 494 GGATCC [GA]^GATC[CT]
613 AGATCC
MspA1I 482 CAGCGG C[AC]G^C[GT]G
633 CAGCTG
RsaI 75 GTAC GT^AC
714 GTAC
StyI 385 CCAAGG C^C[AT][AT]GG
599 CCATGG
XhoII 494 GGATCC [GA]^GATC[CT]
613 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI AlwNI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIV PstI Sau3AI
Sau96I ScrFI SfcI TseI TspRI