Restriction map for
pFN21AE6614
(Circular) (Six-base) MAPSORT of: pFN21AE6614.seq Check: 2533 from: 1 to: 5431
pFN21AE6614 5431 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2309 3241 278
Size: 53 83 186 936 773 932 2468
Fragments arranged by size:
2468 936 932 773 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 3494 4878 5146 1632
Size: 560 1302 1384 268 1917
Fragments arranged by size:
1917 1384 1302 560 268
AfeI AGC'GCT
Cuts at: 2360 2360
Size: 5431
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 2596 2656 2707 3174 4746 2596
Size: 60 51 467 1572 3281
Fragments arranged by size:
3281 1572 467 60 51
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2740 2966 3118 5227 1493
Size: 1247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2300 2756 1870
Size: 73 357 456 4545
Fragments arranged by size:
4545 456 357 73
BamHI G'GATC_C
Cuts at: 2499 2761 3128 2499
Size: 262 367 4802
Fragments arranged by size:
4802 367 262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2752 4207 618
Size: 325 217 627 965 1455 1842
Fragments arranged by size:
1842 1455 965 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2637 2679 2750 1391
Size: 1246 42 71 4072
Fragments arranged by size:
4072 1246 71 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2291 2325 3622 3656 1903
Size: 34 354 34 1297 34 3678
Fragments arranged by size:
3678 1297 354 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 2461 1548
Size: 913 4518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2350 4042 136
Size: 107 122 71 631 605 678 1692 1525
Fragments arranged by size:
1692 1525 678 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2552 2636 2848 2552
Size: 84 212 5135
Fragments arranged by size:
5135 212 84
Bme1580I G_kGCm'C
Cuts at: 1790 2220 3492 4648 1790
Size: 430 1272 1156 2573
Fragments arranged by size:
2573 1272 1156 430
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2532 3162 4120
Size: 416 53 83 399 24 1115 630 958
Cuts at: 4120 442
Size: 1753
Fragments arranged by size:
1753 1115 958 630 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2279 2663 2678 2732 4091 1239
Size: 962 78 384 15 54 1359 2579
Fragments arranged by size:
2579 1359 962 384 78 54 15
Bpu10I CC'TnA_GC
Cuts at: 2543 2543
Size: 5431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4421 4719 3556
Size: 865 298 4268
Fragments arranged by size:
4268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4094 882
Size: 617 2595 2219
Fragments arranged by size:
2595 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3222 493
Size: 711 1263 755 2702
Fragments arranged by size:
2702 1263 755 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2306 3238 3620 4255
Size: 53 83 186 936 773 932 382 635
Cuts at: 4255 275
Size: 1451
Fragments arranged by size:
1451 936 932 773 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2651 2681 1178
Size: 30 165 30 1248 30 3928
Fragments arranged by size:
3928 1248 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2660 2675 2699 2702 1186
Size: 967 507 15 24 3 3915
Fragments arranged by size:
3915 967 507 24 15 3
BseYI C'CCAG_C
Cuts at: 2494 2640 4634 5130 2494
Size: 146 1994 496 2795
Fragments arranged by size:
2795 1994 496 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2043 1163
Size: 573 307 4551
Fragments arranged by size:
4551 573 307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2522 3492 3577 4648 2522
Size: 970 85 1071 3305
Fragments arranged by size:
3305 1071 970 85
BsmI GAATG_Cn'
Cuts at: 2098 2942 3035 2098
Size: 844 93 4494
Fragments arranged by size:
4494 844 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2297 2297
Size: 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2522 2637 2679 2750 3492 3577
Size: 399 430 302 115 42 71 742 85
Cuts at: 3577 4648 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 430 399 302 115 85
71 42
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 844
Size: 1942 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2185 3318 1394
Size: 429 362 1133 3507
Fragments arranged by size:
3507 1133 429 362
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2473 4075 4262 1700
Size: 110 663 1602 187 2869
Fragments arranged by size:
2869 1602 663 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2066 2707 794
Size: 1272 641 3518
Fragments arranged by size:
3518 1272 641
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2499 2618 2761 3128 3513 3530 4982 5068
Size: 527 119 143 367 385 17 1452 86
Cuts at: 5068 5080 5426 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 527 385 367 346 143 119
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2604 513
Size: 552 939 600 3340
Fragments arranged by size:
3340 939 600 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2118 2947 3741 3761 2064
Size: 54 829 794 20 3734
Fragments arranged by size:
3734 829 794 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 2878 3767
Size: 54 1425 50 275 18 432 616 889
Cuts at: 3767 5396 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 616 432 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 3361 1353
Size: 1058 950 3423
Fragments arranged by size:
3423 1058 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4018 4392 4538 387
Size: 1064 138 2429 374 146 1280
Fragments arranged by size:
2429 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2201 2279 2663 2678 2732 3494
Size: 393 560 9 78 384 15 54 762
Cuts at: 3494 4091 4878 5146 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 762 597 560 393 384 268
78 54 15 9
EcoO109I rG'GnC_Cy
Cuts at: 2247 2247
Size: 5431
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2362 2530 4578 1678
Size: 684 168 2048 2531
Fragments arranged by size:
2531 2048 684 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 4072 4104 4146
Size: 32 135 32 1079 32 1389 32 42
Cuts at: 4146 4178 1373
Size: 32 2626
Fragments arranged by size:
2626 1389 1079 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 2775 3021 669
Size: 988 281 790 47 246 3079
Fragments arranged by size:
3079 988 790 281 246 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4729 1305
Size: 3240 184 2007
Fragments arranged by size:
3240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 3177
Fragments arranged by size:
3177 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3748 3907 518
Size: 648 2223 359 159 2042
Fragments arranged by size:
2223 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2489 2640 2847 3524 4672 4917 1606
Size: 883 151 207 677 1148 245 2120
Fragments arranged by size:
2120 1148 883 677 245 207 151
NaeI GCC'GGC
Cuts at: 2475 2475
Size: 5431
NcoI C'CATG_G
Cuts at: 513 1065 2004 2604 513
Size: 552 939 600 3340
Fragments arranged by size:
3340 939 600 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NgoMIV G'CCGG_C
Cuts at: 2473 2473
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2789 4334 1132
Size: 97 1560 1545 2229
Fragments arranged by size:
2229 1560 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 1108
Size: 1152 4279
PfoI T'CCnGG_A
Cuts at: 2253 2679 2253
Size: 426 5005
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PmlI CAC'GTG
Cuts at: 2467 2467
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 5013 5045 3500
Size: 32 1481 32 3886
Fragments arranged by size:
3886 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2247
Size: 5431
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2055 2379 2703 2783 830
Size: 1225 324 324 80 3478
Fragments arranged by size:
3478 1225 324 324 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
PvuII CAG'CTG
Cuts at: 2640 2640
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2703 2783 2703
Size: 80 5351
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SexAI A'CCwGG_T
Cuts at: 2721 2721
Size: 5431
SfcI C'TryA_G
Cuts at: 826 1045 2051 2375 2699 2779 3914 4595 4786
Size: 219 1006 324 324 80 1135 681 191
Cuts at: 4786 826
Size: 1471
Fragments arranged by size:
1471 1135 1006 681 324 324 219 191
80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3535 4436 4698 820
Size: 197 926 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2004 2390 2604 2870 513
Size: 552 939 386 214 266 3074
Fragments arranged by size:
3074 939 552 386 266 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 3475 3628 3813 4258 325
Size: 1255 1878 17 153 185 445 1498
Fragments arranged by size:
1878 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 3677 5389
Size: 274 80 33 51 1443 102 1598 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1598 1443 274 138 102 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5391 5423 5391
Size: 32 5399
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 3560 3560
Size: 5431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2307 3239 276
Size: 53 83 186 936 773 932 2468
Fragments arranged by size:
2468 936 932 773 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI