(Linear) (Six-base) MAPSORT of: KIEE6614.seq Check: 4099 from: 1 to: 726 KIEE6614.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AatII G_ACGT'C Cuts at: 0 304 726 Size: 304 422 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 726 Size: 187 539 AfeI AGC'GCT Cuts at: 0 355 726 Size: 355 371 AlwNI CAG_nnn'CTG Cuts at: 0 591 651 702 726 Size: 591 60 51 24 Fragments arranged by size: 591 60 51 24 AvaI C'yCGr_G Cuts at: 0 295 726 Size: 295 431 BamHI G'GATC_C Cuts at: 0 494 726 Size: 494 232 BanII G_rGCy'C Cuts at: 0 632 674 726 Size: 632 42 52 Fragments arranged by size: 632 52 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 286 320 726 Size: 286 34 406 Fragments arranged by size: 406 286 34 BclI T'GATC_A Cuts at: 0 456 726 Size: 456 270 BglI GCCn_nnn'nGGC Cuts at: 0 345 726 Size: 345 381 BlpI GC'TnA_GC Cuts at: 0 547 631 726 Size: 547 84 95 Fragments arranged by size: 547 95 84 Bme1580I G_kGCm'C Cuts at: 0 215 726 Size: 215 511 BmrI ACTGGGnnnn_n' Cuts at: 0 527 726 Size: 527 199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 274 658 673 726 Size: 196 78 384 15 53 Fragments arranged by size: 384 196 78 53 15 Bpu10I CC'TnA_GC Cuts at: 0 538 726 Size: 538 188 BsaAI yAC'GTr Cuts at: 0 462 726 Size: 462 264 BsaHI Gr'CG_yC Cuts at: 0 301 726 Size: 301 425 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 646 676 726 Size: 646 30 50 Fragments arranged by size: 646 50 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 148 655 670 694 697 726 Size: 148 507 15 24 3 29 Fragments arranged by size: 507 148 29 24 15 3 BseYI C'CCAG_C Cuts at: 0 489 635 726 Size: 489 146 91 Fragments arranged by size: 489 146 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 726 Size: 38 688 BsiHKAI G_wGCw'C Cuts at: 0 517 726 Size: 517 209 BsmI GAATG_Cn' Cuts at: 0 93 726 Size: 93 633 BsmBI CGTCTCn'nnnn_ Cuts at: 0 292 726 Size: 292 434 Bsp1286I G_dGCh'C Cuts at: 0 215 517 632 674 726 Size: 215 302 115 42 52 Fragments arranged by size: 302 215 115 52 42 BsrBI CCG'CTC Cuts at: 0 180 726 Size: 180 546 BsrFI r'CCGG_y Cuts at: 0 468 726 Size: 468 258 BstAPI GCAn_nnn'nTGC Cuts at: 0 61 702 726 Size: 61 641 24 Fragments arranged by size: 641 61 24 BstYI r'GATC_y Cuts at: 0 494 613 726 Size: 494 119 113 Fragments arranged by size: 494 119 113 BtgI C'CryG_G Cuts at: 0 599 726 Size: 599 127 BtsI GCAGTG_nn' Cuts at: 0 59 113 726 Size: 59 54 613 Fragments arranged by size: 613 59 54 EaeI y'GGCC_r Cuts at: 0 257 726 Size: 257 469 EarI CTCTTCn'nnn_ Cuts at: 0 406 726 Size: 406 320 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 196 274 658 673 726 Size: 187 9 78 384 15 53 Fragments arranged by size: 384 187 78 53 15 9 EcoO109I rG'GnC_Cy Cuts at: 0 242 726 Size: 242 484 HaeII r_GCGC'y Cuts at: 0 357 525 726 Size: 357 168 201 Fragments arranged by size: 357 201 168 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 646 678 726 Size: 646 32 48 Fragments arranged by size: 646 48 32 HincII GTy'rAC Cuts at: 0 723 726 Size: 723 3 MscI TGG'CCA Cuts at: 0 259 726 Size: 259 467 MspA1I CmG'CkG Cuts at: 0 484 635 726 Size: 484 151 91 Fragments arranged by size: 484 151 91 NaeI GCC'GGC Cuts at: 0 470 726 Size: 470 256 NcoI C'CATG_G Cuts at: 0 599 726 Size: 599 127 NgoMIV G'CCGG_C Cuts at: 0 468 726 Size: 468 258 PflMI CCAn_nnn'nTGG Cuts at: 0 255 726 Size: 255 471 PfoI T'CCnGG_A Cuts at: 0 248 674 726 Size: 248 426 52 Fragments arranged by size: 426 248 52 PmlI CAC'GTG Cuts at: 0 462 726 Size: 462 264 PpuMI rG'GwC_Cy Cuts at: 0 242 726 Size: 242 484 PstI C_TGCA'G Cuts at: 0 50 374 698 726 Size: 50 324 324 28 Fragments arranged by size: 324 324 50 28 PvuII CAG'CTG Cuts at: 0 635 726 Size: 635 91 SbfI CC_TGCA'GG Cuts at: 0 698 726 Size: 698 28 SexAI A'CCwGG_T Cuts at: 0 716 726 Size: 716 10 SfcI C'TryA_G Cuts at: 0 46 370 694 726 Size: 46 324 324 32 Fragments arranged by size: 324 324 46 32 StyI C'CwwG_G Cuts at: 0 385 599 726 Size: 385 214 127 Fragments arranged by size: 385 214 127 TatI w'GTAC_w Cuts at: 0 74 726 Size: 74 652 ZraI GAC'GTC Cuts at: 0 302 726 Size: 302 424 Enzymes that do cut: AatII AcuI AfeI AlwNI AvaI BamHI BanII BcgI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BstAPI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII MscI MspA1I NaeI NcoI NgoMIV PflMI PfoI PmlI PpuMI PstI PvuII SbfI SexAI SfcI StyI TatI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BciVI BfrBI BglII BmgBI BmtI BplI BpuEI BsaI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI