Restriction map for
pFN21AE1989
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwNI AocI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbrPI
BbvCI BciVI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
Eam1105I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I HincII HindII HindIII
HinfI HpaI KpnI KspI MamI
MfeI MroI MseI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 721 ACGCGT A^C[GA][CT]GT
Asp700I 375 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1379 TGCGCA TGC^GCA
BamHI 740 GGATCC G^GATCC
BanII 476 GAGCTC G[GA]GC[CT]^C
BbeI 433 GGCGCC GGCGC^C
BbsI 1390 GTCTTC GAAGAC(2/6)
BcgI 140 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1565 TGATCA T^GATCA
BglII 443 AGATCT A^GATCT
BlpI 1191 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1191 GCTGAGC GC^T[ACGT]AGC
BpuAI 1390 GTCTTC GAAGAC(2/6)
BsaAI 792 CACGTA [CT]AC^GT[GA]
BsaI 1684 GAGACC GGTCTC(1/5)
BseRI 715 GAGGAG GAGGAG(10/8)
BsiEI 1139 CGGCCG CG[GA][CT]^CG
BsmBI 718 GAGACG CGTCTC(1/5)
BsmFI 510 GGGAC GGGAC(10/14)
BstEII 940 GGTTACC G^GT[ACGT]ACC
BstPI 940 GGTTACC G^GT[ACGT]ACC
CelII 1191 GCTGAGC GC^T[ACGT]AGC
DrdI 1549 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1139 CGGCCG C^GGCCG
EclXI 1139 CGGCCG C^GGCCG
Eco52I 1139 CGGCCG C^GGCCG
EcoO65I 940 GGTTACC G^GT[ACGT]ACC
FbaI 1565 TGATCA T^GATCA
FseI 62 GGCCGGCC GGCCGG^CC
FspI 1379 TGCGCA TGC^GCA
KasI 433 GGCGCC G^GCGCC
MluI 721 ACGCGT A^CGCGT
NarI 433 GGCGCC GG^CGCC
NheI 1248 GCTAGC G^CTAGC
PflMI 1025 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 514 CTGCAG CTGCA^G
SacI 476 GAGCTC GAGCT^C
ScaI 1109 AGTACT AGT^ACT
SfcI 514 CTGCAG C^T[GA][CT]AG
SfoI 433 GGCGCC GGC^GCC
SmlI 613 CTCAAG C^T[CT][GA]AG
Tsp45I 787 GTCAC ^GT[GC]AC
Van91I 1025 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 375 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 802 GTAGAC GT^[AC][GT]AC
1558 GTCTAC
AcyI 433 GGCGCC G[GA]^CG[CT]C
1387 GGCGTC
AvaII 102 GGACC G^G[AT]CC
1227 GGACC
BfaI 211 CTAG C^TAG
1249 CTAG
BsaHI 433 GGCGCC G[GA]^CG[CT]C
1387 GGCGTC
BsmAI 718 GAGAC GTCTC(1/5)
1684 GAGAC
BspMI 678 ACCTGC ACCTGC(4/8)
1187 ACCTGC
BstXI 173 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
791 CCACGTACTTGG
EcoT14I 863 CCATGG C^C[AT][AT]GG
1166 CCATGG
Hin1I 433 GGCGCC G[GA]^CG[CT]C
1387 GGCGTC
MaeI 211 CTAG C^TAG
1249 CTAG
MaeIII 787 GTCAC ^GT[ACGT]AC
941 GTTAC
NcoI 863 CCATGG C^CATGG
1166 CCATGG
NspI 806 ACATGC [GA]CATG^[CT]
1538 GCATGT
SapI 1257 GCTCTTC GCTCTTC(1/4)
1311 GCTCTTC
SexAI 748 ACCTGGT A^CC[AT]GGT
1051 ACCTGGT
SmaI 537 CCCGGG CCC^GGG
546 CCCGGG
StyI 863 CCATGG C^C[AT][AT]GG
1166 CCATGG
TaqI 1094 TCGA T^CGA
1178 TCGA
TthHB8I 1094 TCGA T^CGA
1178 TCGA
XmaCI 537 CCCGGG C^CCGGG
546 CCCGGG
XmaI 537 CCCGGG C^CCGGG
546 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI Aor51HI ApaLI AspHI AvaI
BalI BanI BbvI BcnI BglI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BspHI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI Eco47III
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfiI
StuI TseI TspRI XcmI XhoII