Restriction map for
pFN21AE6569
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BseRI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DpnII DraI DraII DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 456 GGCGCC G[GA]^CG[CT]C
AflIII 221 ACGTGT A^C[GA][CT]GT
AlwNI 433 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 385 GGGCCC GGGCC^C
AscI 333 GGCGCGCC GG^CGCGCC
AvaII 548 GGACC G^G[AT]CC
BanI 456 GGCGCC G^G[CT][GA]CC
BbeI 456 GGCGCC GGCGC^C
BfaI 380 CTAG C^TAG
BsaAI 262 TACGTG [CT]AC^GT[GA]
BsaHI 456 GGCGCC G[GA]^CG[CT]C
BsaI 211 GAGACC GGTCTC(1/5)
BsaWI 141 ACCGGA [AT]^CCGG[AT]
BsrBI 557 CCGCTC CCGCTC(-3/-3)
BsrFI 294 GCCGGT [GA]^CCGG[CT]
BssSI 489 CTCGTG CACGAG(-5/-1)
BstAPI 423 GCAGCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 297 GGTCACC G^GT[ACGT]ACC
BstPI 297 GGTCACC G^GT[ACGT]ACC
Cfr10I 294 GCCGGT [GA]^CCGG[CT]
EcoO65I 297 GGTCACC G^GT[ACGT]ACC
Hin1I 456 GGCGCC G[GA]^CG[CT]C
KasI 456 GGCGCC G^GCGCC
MaeI 380 CTAG C^TAG
NarI 456 GGCGCC GG^CGCC
NlaIII 165 CATG CATG^
SfiI 319 GGCCAGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 456 GGCGCC GGC^GCC
SmlI 535 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 112 CCCGAG C^[CT]CG[GA]G
130 CCCGAG
BglI 320 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
610 GCCAACCAGGC
BpmI 148 CTGGAG CTGGAG(16/14)
625 CTGGAG
BsgI 247 CTGCAC GTGCAG(16/14)
430 GTGCAG
BsmAI 211 GAGAC GTCTC(1/5)
23 GTCTC
BsmFI 547 GGGAC GGGAC(10/14)
443 GTCCC
BsoBI 112 CCCGAG C^[CT]CG[GA]G
130 CCCGAG
BstF5I 108 CATCC GGATG(2/0)
402 CATCC
EagI 357 CGGCCG C^GGCCG
562 CGGCCG
EclXI 357 CGGCCG C^GGCCG
562 CGGCCG
Eco52I 357 CGGCCG C^GGCCG
562 CGGCCG
FokI 108 CATCC GGATG(9/13)
402 CATCC
KspI 360 CCGCGG CCGC^GG
583 CCGCGG
MaeII 221 ACGT A^CGT
263 ACGT
MaeIII 265 GTGAC ^GT[ACGT]AC
298 GTCAC
PvuII 234 CAGCTG CAG^CTG
433 CAGCTG
SacI 115 GAGCTC GAGCT^C
532 GAGCTC
SacII 360 CCGCGG CCGC^GG
583 CCGCGG
SfaNI 94 GATGC GCATC(5/9)
401 GCATC
Tsp45I 265 GTGAC ^GT[GC]AC
298 GTCAC
TspRI 11 CACTG CA[GC]TG[ACGT][ACGT]^
322 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanII BbvI BcnI BmyI BsaJI
BsiEI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I DsaI EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV RsaI
Sau96I ScrFI TseI