(Circular) (Six-base) MAPSORT of: pFN21AE6569.seq Check: 6139 from: 1 to: 5344 pFN21AE6569 5344 bp With 182 enzymes: SgfI * December 25, 2014 18:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3154 278 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2687 1937 Size: 750 4594 Acc65I G'GTAC_C Cuts at: 2665 2665 Size: 5344 AclI AA'CG_TT Cuts at: 1563 3471 3844 1563 Size: 1908 373 3063 Fragments arranged by size: 3063 1908 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3407 4791 5059 1632 Size: 1775 1384 268 1917 Fragments arranged by size: 1917 1775 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5147 AflIII A'CryG_T Cuts at: 1203 2226 4243 5220 1203 Size: 1023 2017 977 1327 Fragments arranged by size: 2017 1327 1023 977 AgeI A'CCGG_T Cuts at: 4175 4175 Size: 5344 AhdI GACnn_n'nnGTC Cuts at: 4073 4073 Size: 5344 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5344 AlwNI CAG_nnn'CTG Cuts at: 2443 3087 4659 2443 Size: 644 1572 3128 Fragments arranged by size: 3128 1572 644 ApaI G_GGCC'C Cuts at: 2394 2394 Size: 5344 ApaLI G'TGCA_C Cuts at: 3401 4557 3401 Size: 1156 4188 ApoI r'AATT_y Cuts at: 1493 2653 2879 3031 5140 1493 Size: 1160 226 152 2109 1697 Fragments arranged by size: 2109 1697 1160 226 152 AscI GG'CGCG_CC Cuts at: 2339 2339 Size: 5344 AseI AT'TA_AT Cuts at: 160 1032 3898 160 Size: 872 2866 1606 Fragments arranged by size: 2866 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2135 2669 1870 Size: 73 174 18 534 4545 Fragments arranged by size: 4545 534 174 73 18 BamHI G'GATC_C Cuts at: 2674 3041 2674 Size: 367 4977 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2461 2665 4120 618 Size: 325 217 627 674 204 1455 1842 Fragments arranged by size: 1842 1455 674 627 325 217 204 BanII G_rGCy'C Cuts at: 1391 2124 2394 2541 2663 1391 Size: 733 270 147 122 4072 Fragments arranged by size: 4072 733 270 147 122 BbeI G_GCGC'C Cuts at: 2465 2465 Size: 5344 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4373 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3535 3569 1903 Size: 34 50 34 1514 34 3678 Fragments arranged by size: 3678 1514 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3236 4446 3236 Size: 1210 4134 BclI T'GATC_A Cuts at: 1548 1548 Size: 5344 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2331 2621 3955 Size: 107 122 71 631 605 659 290 1334 Cuts at: 3955 136 Size: 1525 Fragments arranged by size: 1525 1334 659 631 605 290 122 107 71 BglII A'GATC_T Cuts at: 5339 5339 Size: 5344 BlpI GC'TnA_GC Cuts at: 2761 2761 Size: 5344 Bme1580I G_kGCm'C Cuts at: 1790 2394 3405 4561 1790 Size: 604 1011 1156 2573 Fragments arranged by size: 2573 1156 1011 604 BmgBI CAC'GTC Cuts at: 1318 2227 1318 Size: 909 4435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3075 4033 442 Size: 416 53 83 399 24 1658 958 1753 Fragments arranged by size: 1753 1658 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2651 4004 1239 Size: 935 477 1353 2579 Fragments arranged by size: 2579 1353 935 477 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 3469 4334 4632 2525 Size: 944 865 298 3237 Fragments arranged by size: 3237 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2210 4007 882 Size: 617 711 1797 2219 Fragments arranged by size: 2219 1797 711 617 BsaAI yAC'GTr Cuts at: 493 1204 2269 3135 493 Size: 711 1065 866 2702 Fragments arranged by size: 2702 1065 866 711 BsaBI GATnn'nnATC Cuts at: 5338 5338 Size: 5344 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2462 3151 3533 4168 Size: 53 83 186 936 929 689 382 635 Cuts at: 4168 275 Size: 1451 Fragments arranged by size: 1451 936 929 689 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2146 3777 4175 4449 4596 5007 2146 Size: 1631 398 274 147 411 2483 Fragments arranged by size: 2483 1631 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5119 Fragments arranged by size: 5119 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5344 BseYI C'CCAG_C Cuts at: 4547 5043 4547 Size: 496 4848 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2456 1163 Size: 573 501 219 4051 Fragments arranged by size: 4051 573 501 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2365 2416 2570 2794 3555 4583 Size: 275 186 364 51 154 224 761 1028 Cuts at: 4583 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 364 275 224 186 154 51 BsiHKAI G_wGCw'C Cuts at: 2124 2321 2541 3405 3490 4561 2124 Size: 197 220 864 85 1071 2907 Fragments arranged by size: 2907 1071 864 220 197 85 BsmI GAATG_Cn' Cuts at: 2855 2948 2855 Size: 93 5251 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2321 2394 2541 2663 3405 3490 Size: 399 334 197 73 147 122 742 85 Cuts at: 3490 4561 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 197 147 122 85 73 BspEI T'CCGG_A Cuts at: 5007 5007 Size: 5344 BspHI T'CATG_A Cuts at: 3233 4963 3233 Size: 1730 3614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2699 844 Size: 1855 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2564 3231 1394 Size: 429 741 667 3507 Fragments arranged by size: 3507 741 667 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3839 4013 65 Size: 1183 2591 174 1396 Fragments arranged by size: 2591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2299 3988 4175 1700 Size: 110 489 1689 187 2869 Fragments arranged by size: 2869 1689 489 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5344 BssHII G'CGCG_C Cuts at: 1925 2339 2424 2467 2469 2471 1925 Size: 414 85 43 2 2 4798 Fragments arranged by size: 4798 414 85 43 2 2 BssSI C'ACGA_G Cuts at: 2494 3404 4416 2494 Size: 910 1012 3422 Fragments arranged by size: 3422 1012 910 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 794 Size: 1640 3704 BstBI TT'CG_AA Cuts at: 4148 4148 Size: 5344 BstEII G'GTnAC_C Cuts at: 1184 2302 1184 Size: 1118 4226 BstYI r'GATC_y Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339 Size: 702 367 385 17 1452 86 12 346 Cuts at: 5339 1972 Size: 1977 Fragments arranged by size: 1977 1452 702 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2103 2365 2588 513 Size: 552 1038 262 223 3269 Fragments arranged by size: 3269 1038 552 262 223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4605 Fragments arranged by size: 4605 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3654 3674 2860 Size: 794 20 4530 Fragments arranged by size: 4530 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5344 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5309 DraI TTT'AAA Cuts at: 2648 2648 Size: 5344 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4351 809 Size: 507 3035 1802 Fragments arranged by size: 3035 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2323 2362 2567 Size: 54 1425 50 275 18 493 39 205 Cuts at: 2567 2791 3680 5309 8 Size: 224 889 1629 43 Fragments arranged by size: 1629 1425 889 493 275 224 205 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2362 2567 2791 1537 Size: 275 550 205 224 4090 Fragments arranged by size: 4090 550 275 224 205 EarI CTCTTCn'nnn_ Cuts at: 1353 3274 1353 Size: 1921 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2195 3931 4305 4451 387 Size: 1064 138 606 1736 374 146 1280 Fragments arranged by size: 1736 1280 1064 606 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2539 2122 Size: 417 4927 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2651 3407 4004 4791 5059 1239 Size: 393 542 477 756 597 787 268 1524 Fragments arranged by size: 1524 787 756 597 542 477 393 268 EcoRI G'AATT_C Cuts at: 2653 3031 2653 Size: 378 4966 FspI TGC'GCA Cuts at: 3850 3850 Size: 5344 HaeII r_GCGC'y Cuts at: 1678 2295 2465 2512 2610 4491 1678 Size: 617 170 47 98 1881 2531 Fragments arranged by size: 2531 1881 617 170 98 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373 Size: 32 135 32 2413 32 42 32 2626 Fragments arranged by size: 2626 2413 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2688 2934 669 Size: 988 281 750 246 3079 Fragments arranged by size: 3079 988 750 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5344 HpaI GTT'AAC Cuts at: 2934 2934 Size: 5344 KasI G'GCGC_C Cuts at: 2461 2461 Size: 5344 KpnI G_GTAC'C Cuts at: 2669 2669 Size: 5344 MfeI C'AATT_G Cuts at: 2943 2943 Size: 5344 MluI A'CGCG_T Cuts at: 5220 5220 Size: 5344 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 4458 4642 1305 Size: 1209 1944 184 2007 Fragments arranged by size: 2007 1944 1209 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3522 Fragments arranged by size: 3522 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3302 3661 3820 518 Size: 648 2136 359 159 2042 Fragments arranged by size: 2136 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2241 2367 2403 2440 2590 2760 3437 4585 Size: 635 126 36 37 150 170 677 1148 Cuts at: 4585 4830 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 635 245 170 150 126 37 36 NarI GG'CG_CC Cuts at: 2462 2462 Size: 5344 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4792 NdeI CA'TA_TG Cuts at: 387 387 Size: 5344 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5344 NotI GC'GGCC_GC Cuts at: 2791 2791 Size: 5344 NspI r_CATG'y Cuts at: 1132 1229 2702 4247 1132 Size: 97 1473 1545 2229 Fragments arranged by size: 2229 1545 1473 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5344 PciI A'CATG_T Cuts at: 4243 4243 Size: 5344 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5344 PmeI GTTT'AAAC Cuts at: 2648 2648 Size: 5344 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3413 3445 4926 4958 3413 Size: 32 1481 32 3799 Fragments arranged by size: 3799 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5344 PsiI TTA'TAA Cuts at: 2914 2914 Size: 5344 PspOMI G'GGCC_C Cuts at: 2390 2390 Size: 5344 PstI C_TGCA'G Cuts at: 830 2696 830 Size: 1866 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5344 PvuII CAG'CTG Cuts at: 2241 2440 2241 Size: 199 5145 SacI G_AGCT'C Cuts at: 2124 2541 2124 Size: 417 4927 SacII CC_GC'GG Cuts at: 2368 2591 2368 Size: 223 5121 SalI G'TCGA_C Cuts at: 1936 2686 1936 Size: 750 4594 SbfI CC_TGCA'GG Cuts at: 2696 2696 Size: 5344 ScaI AGT'ACT Cuts at: 3592 5304 3592 Size: 1712 3632 SfcI C'TryA_G Cuts at: 826 1045 2692 3827 4508 4699 826 Size: 219 1647 1135 681 191 1471 Fragments arranged by size: 1647 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2331 2331 Size: 5344 SfoI GGC'GCC Cuts at: 2463 2463 Size: 5344 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5344 SmaI CCC'GGG Cuts at: 1872 2671 1872 Size: 799 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 3448 4349 4611 820 Size: 197 926 597 908 901 262 1553 Fragments arranged by size: 1553 926 908 901 597 262 197 SnaBI TAC'GTA Cuts at: 493 3135 493 Size: 2642 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5344 SphI G_CATG'C Cuts at: 1132 2702 1132 Size: 1570 3774 SspI AAT'ATT Cuts at: 5 52 3268 5 Size: 47 3216 2081 Fragments arranged by size: 3216 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5344 StyI C'CwwG_G Cuts at: 513 1065 2783 513 Size: 552 1718 3074 Fragments arranged by size: 3074 1718 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2288 2570 3371 3388 3541 3726 4171 Size: 1255 708 282 801 17 153 185 445 Cuts at: 4171 325 Size: 1498 Fragments arranged by size: 1498 1255 801 708 445 282 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 3590 5302 Size: 274 80 33 51 1443 258 1355 1712 Cuts at: 5302 96 Size: 138 Fragments arranged by size: 1712 1443 1355 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5304 5336 5304 Size: 32 5312 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5344 XbaI T'CTAG_A Cuts at: 2680 2680 Size: 5344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5344 XmaI C'CCGG_G Cuts at: 1870 2669 1870 Size: 799 4545 XmnI GAAnn'nnTTC Cuts at: 3473 3473 Size: 5344 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3152 276 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI XcmI