(Linear) (Six-base) MAPSORT of: KIEE6569.seq Check: 4540 from: 1 to: 639 KIEE6569.orf With 182 enzymes: SgfI * December 25, 2014 17:19 .. AflIII A'CryG_T Cuts at: 0 221 639 Size: 221 418 AlwNI CAG_nnn'CTG Cuts at: 0 438 639 Size: 438 201 ApaI G_GGCC'C Cuts at: 0 389 639 Size: 389 250 AscI GG'CGCG_CC Cuts at: 0 334 639 Size: 334 305 AvaI C'yCGr_G Cuts at: 0 112 130 639 Size: 112 18 509 Fragments arranged by size: 509 112 18 BanI G'GyrC_C Cuts at: 0 456 639 Size: 456 183 BanII G_rGCy'C Cuts at: 0 119 389 536 639 Size: 119 270 147 103 Fragments arranged by size: 270 147 119 103 BbeI G_GCGC'C Cuts at: 0 460 639 Size: 460 179 BglI GCCn_nnn'nGGC Cuts at: 0 326 616 639 Size: 326 290 23 Fragments arranged by size: 326 290 23 Bme1580I G_kGCm'C Cuts at: 0 389 639 Size: 389 250 BmgBI CAC'GTC Cuts at: 0 222 639 Size: 222 417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 639 Size: 169 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 639 Size: 520 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 205 639 Size: 205 434 BsaAI yAC'GTr Cuts at: 0 264 639 Size: 264 375 BsaHI Gr'CG_yC Cuts at: 0 457 639 Size: 457 182 BsaWI w'CCGG_w Cuts at: 0 141 639 Size: 141 498 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 451 639 Size: 232 219 188 Fragments arranged by size: 232 219 188 BsiEI CG_ry'CG Cuts at: 0 360 411 565 639 Size: 360 51 154 74 Fragments arranged by size: 360 154 74 51 BsiHKAI G_wGCw'C Cuts at: 0 119 316 536 639 Size: 119 197 220 103 Fragments arranged by size: 220 197 119 103 Bsp1286I G_dGCh'C Cuts at: 0 119 316 389 536 639 Size: 119 197 73 147 103 Fragments arranged by size: 197 147 119 103 73 BsrBI CCG'CTC Cuts at: 0 559 639 Size: 559 80 BsrFI r'CCGG_y Cuts at: 0 294 639 Size: 294 345 BssHII G'CGCG_C Cuts at: 0 334 419 462 464 466 639 Size: 334 85 43 2 2 173 Fragments arranged by size: 334 173 85 43 2 2 BssSI C'ACGA_G Cuts at: 0 489 639 Size: 489 150 BstAPI GCAn_nnn'nTGC Cuts at: 0 429 639 Size: 429 210 BstEII G'GTnAC_C Cuts at: 0 297 639 Size: 297 342 BtgI C'CryG_G Cuts at: 0 98 360 583 639 Size: 98 262 223 56 Fragments arranged by size: 262 223 98 56 EaeI y'GGCC_r Cuts at: 0 318 357 562 639 Size: 318 39 205 77 Fragments arranged by size: 318 205 77 39 EagI C'GGCC_G Cuts at: 0 357 562 639 Size: 357 205 77 Fragments arranged by size: 357 205 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 190 639 Size: 190 449 EcoICRI GAG'CTC Cuts at: 0 117 534 639 Size: 117 417 105 Fragments arranged by size: 417 117 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 639 Size: 169 470 HaeII r_GCGC'y Cuts at: 0 290 460 507 605 639 Size: 290 170 47 98 34 Fragments arranged by size: 290 170 98 47 34 KasI G'GCGC_C Cuts at: 0 456 639 Size: 456 183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 509 639 Size: 509 130 MspA1I CmG'CkG Cuts at: 0 236 362 398 435 585 639 Size: 236 126 36 37 150 54 Fragments arranged by size: 236 150 126 54 37 36 NarI GG'CG_CC Cuts at: 0 457 639 Size: 457 182 PspOMI G'GGCC_C Cuts at: 0 385 639 Size: 385 254 PvuII CAG'CTG Cuts at: 0 236 435 639 Size: 236 199 204 Fragments arranged by size: 236 204 199 SacI G_AGCT'C Cuts at: 0 119 536 639 Size: 119 417 103 Fragments arranged by size: 417 119 103 SacII CC_GC'GG Cuts at: 0 363 586 639 Size: 363 223 53 Fragments arranged by size: 363 223 53 SfiI GGCCn_nnn'nGGCC Cuts at: 0 326 639 Size: 326 313 SfoI GGC'GCC Cuts at: 0 458 639 Size: 458 181 SmlI C'TyrA_G Cuts at: 0 535 639 Size: 535 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 283 565 639 Size: 283 282 74 Fragments arranged by size: 283 282 74 TatI w'GTAC_w Cuts at: 0 230 639 Size: 230 409 Enzymes that do cut: AflIII AlwNI ApaI AscI AvaI BanI BanII BbeI BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI BstEII BtgI EaeI EagI EciI EcoICRI Eco57MI HaeII KasI MmeI MspA1I NarI PspOMI PvuII SacI SacII SfiI SfoI SmlI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaBI BsaXI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI