Restriction map for
pFN21AE5785
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbvCI BclI BfrI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KasI Ksp632I KspI
MamI MfeI MluI MroI MseI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1886 GGTACC G^GTACC
AcsI 1122 AAATTC [GA]^AATT[CT]
AcyI 503 GACGCC G[GA]^CG[CT]C
ApaI 1961 GGGCCC GGGCC^C
ApoI 1122 AAATTC [GA]^AATT[CT]
Asp700I 786 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1886 GGTACC G^GTACC
BanI 1886 GGTACC G^G[CT][GA]CC
BbrPI 724 CACGTG CAC^GTG
BcgI 2250 CGACCTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1425 GGATAC GTATCC(6/5)
BglI 361 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1821 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1821 GCTCAGC GC^T[ACGT]AGC
BsaHI 503 GACGCC G[GA]^CG[CT]C
BsaI 1999 GAGACC GGTCTC(1/5)
BsgI 718 CTGCAC GTGCAG(16/14)
BsmAI 1999 GAGAC GTCTC(1/5)
BsrFI 847 GCCGGC [GA]^CCGG[CT]
CelII 1821 GCTCAGC GC^T[ACGT]AGC
Cfr10I 847 GCCGGC [GA]^CCGG[CT]
DraIII 721 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 277 GACAACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 846 GGCCGGCC GGCCGG^CC
Hin1I 503 GACGCC G[GA]^CG[CT]C
HincII 2206 GTCAAC GT[CT]^[GA]AC
HindII 2206 GTCAAC GT[CT]^[GA]AC
KpnI 1886 GGTACC GGTAC^C
NaeI 847 GCCGGC GCC^GGC
NgoMIV 847 GCCGGC G^CCGGC
PmaCI 724 CACGTG CAC^GTG
PmlI 724 CACGTG CAC^GTG
PpuMI 892 GGGTCCT [GA]G^G[AT]CC[CT]
SpeI 1074 ACTAGT A^CTAGT
StuI 1100 AGGCCT AGG^CCT
TaqI 984 TCGA T^CGA
TfiI 1463 GATTC G^A[AT]TC
Tsp509I 1123 AATT ^AATT
TthHB8I 984 TCGA T^CGA
XcmI 47 CCATCTTTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 786 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BglII 1335 AGATCT A^GATCT
1387 AGATCT
BsmI 1027 GCATTC GAATGC(1/-1)
1492 GCATTC
BspMI 357 ACCTGC ACCTGC(4/8)
1686 GCAGGT
BssSI 19 CTCGTG CACGAG(-5/-1)
1732 CTCGTG
BstAPI 1668 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1677 GCAGAGGCTGC
BstEII 1689 GGTGACC G^GT[ACGT]ACC
1792 GGTCACC
BstPI 1689 GGTGACC G^GT[ACGT]ACC
1792 GGTCACC
EcoNI 1092 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2223 CCTGCCCCAGG
EcoO65I 1689 GGTGACC G^GT[ACGT]ACC
1792 GGTCACC
HgaI 503 GACGC GACGC(5/10)
474 GCGTC
NcoI 1643 CCATGG C^CATGG
1859 CCATGG
NspI 452 ACATGC [GA]CATG^[CT]
2058 ACATGC
PflMI 228 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
874 CCAGACAATGG
PleI 2161 GACTC GAGTC(4/5)
1252 GAGTC
SexAI 620 ACCTGGT A^CC[AT]GGT
673 ACCTGGT
Van91I 228 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
874 CCAGACAATGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TseI
Tsp45I TspRI XhoII XmaCI XmaI