(Circular) (Six-base) MAPSORT of: pFN21AE5785.seq Check: 6963 from: 1 to: 6994 pFN21AE5785 6994 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2371 3682 2371 Size: 1311 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4804 278 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 1937 Size: 2400 4594 Acc65I G'GTAC_C Cuts at: 3891 4315 3891 Size: 424 6570 AclI AA'CG_TT Cuts at: 1563 4293 5121 5494 1563 Size: 2730 828 373 3063 Fragments arranged by size: 3063 2730 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2930 2956 3862 5057 6441 6709 1632 Size: 1298 26 906 1195 1384 268 1917 Fragments arranged by size: 1917 1384 1298 1195 906 268 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6797 AflIII A'CryG_T Cuts at: 1203 5893 6870 1203 Size: 4690 977 1327 Fragments arranged by size: 4690 1327 977 AgeI A'CCGG_T Cuts at: 5825 5825 Size: 6994 AhdI GACnn_n'nnGTC Cuts at: 5723 5723 Size: 6994 AleI CACnn'nnGTG Cuts at: 1166 3233 3447 1166 Size: 2067 214 4713 Fragments arranged by size: 4713 2067 214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3839 1184 Size: 2655 4339 AlwNI CAG_nnn'CTG Cuts at: 2239 3688 4091 4737 6309 2239 Size: 1449 403 646 1572 2924 Fragments arranged by size: 2924 1572 1449 646 403 ApaI G_GGCC'C Cuts at: 3970 3970 Size: 6994 ApaLI G'TGCA_C Cuts at: 5051 6207 5051 Size: 1156 5838 ApoI r'AATT_y Cuts at: 1493 3127 4303 4529 4681 6790 1493 Size: 1634 1176 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 1176 226 152 AseI AT'TA_AT Cuts at: 160 1032 5548 160 Size: 872 4516 1606 Fragments arranged by size: 4516 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 AvaI C'yCGr_G Cuts at: 1870 1943 2367 2704 2926 3804 4319 1870 Size: 73 424 337 222 878 515 4545 Fragments arranged by size: 4545 878 515 424 337 222 73 BamHI G'GATC_C Cuts at: 4324 4691 4324 Size: 367 6627 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3891 4315 5770 618 Size: 325 217 627 2104 424 1455 1842 Fragments arranged by size: 2104 1842 1455 627 424 325 217 BanII G_rGCy'C Cuts at: 1391 2357 3668 3930 3970 4313 1391 Size: 966 1311 262 40 343 4072 Fragments arranged by size: 4072 1311 966 343 262 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 2284 2380 3430 4149 928 Size: 971 122 263 96 1050 719 3773 Fragments arranged by size: 3773 1050 971 719 263 122 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4244 4278 5185 5219 1903 Size: 34 2307 34 907 34 3678 Fragments arranged by size: 3678 2307 907 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3424 4886 6096 3424 Size: 1462 1210 4322 Fragments arranged by size: 4322 1462 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6994 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 5605 136 Size: 107 122 71 631 605 700 3233 1525 Fragments arranged by size: 3233 1525 700 631 605 122 107 71 BglII A'GATC_T Cuts at: 3340 3392 6989 3340 Size: 52 3597 3345 Fragments arranged by size: 3597 3345 52 BlpI GC'TnA_GC Cuts at: 3827 4411 3827 Size: 584 6410 Bme1580I G_kGCm'C Cuts at: 1790 2149 2925 3557 3660 3970 4252 5055 6211 Size: 359 776 632 103 310 282 803 1156 Cuts at: 6211 1790 Size: 2573 Fragments arranged by size: 2573 1156 803 776 632 359 310 282 103 BmgBI CAC'GTC Cuts at: 1318 2467 1318 Size: 1149 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2136 2836 3441 Size: 416 53 83 399 24 719 700 605 Cuts at: 3441 3525 3625 3924 4725 5683 442 Size: 84 100 299 801 958 1753 Fragments arranged by size: 1753 958 801 719 700 605 416 399 299 100 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6994 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2855 3629 3682 3731 3858 5654 1239 Size: 1616 774 53 49 127 1796 2579 Fragments arranged by size: 2579 1796 1616 774 127 53 49 Bpu10I CC'TnA_GC Cuts at: 2192 3669 2192 Size: 1477 5517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5119 5984 6282 5119 Size: 865 298 5831 Fragments arranged by size: 5831 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3998 5657 882 Size: 617 2499 1659 2219 Fragments arranged by size: 2499 2219 1659 617 BsaAI yAC'GTr Cuts at: 493 1204 2731 3235 3281 4785 493 Size: 711 1527 504 46 1504 2702 Fragments arranged by size: 2702 1527 1504 711 504 46 BsaBI GATnn'nnATC Cuts at: 6988 6988 Size: 6994 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2509 4801 5183 5818 Size: 53 83 186 936 976 2292 382 635 Cuts at: 5818 275 Size: 1451 Fragments arranged by size: 2292 1451 976 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5427 5825 6099 6246 6657 5427 Size: 398 274 147 411 5764 Fragments arranged by size: 5764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2629 2659 2828 2858 1178 Size: 30 165 30 1226 30 169 30 5314 Fragments arranged by size: 5314 1226 169 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 2216 2989 3734 3935 3938 1186 Size: 835 195 773 745 201 3 4242 Fragments arranged by size: 4242 835 773 745 201 195 3 BseYI C'CCAG_C Cuts at: 2108 2148 2232 2239 2406 2711 2829 3133 3882 Size: 40 84 7 167 305 118 304 749 Cuts at: 3882 3909 4196 6197 6693 2108 Size: 27 287 2001 496 2409 Fragments arranged by size: 2409 2001 749 496 305 304 287 167 118 84 40 27 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2708 1163 Size: 573 972 5449 Fragments arranged by size: 5449 972 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4444 5205 6233 1540 Size: 275 186 2443 761 1028 2301 Fragments arranged by size: 2443 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 3546 3675 5055 5140 6211 2031 Size: 1515 129 1380 85 1071 2814 Fragments arranged by size: 2814 1515 1380 1071 129 85 BsmI GAATG_Cn' Cuts at: 3032 3497 4505 4598 3032 Size: 465 1008 93 5428 Fragments arranged by size: 5428 1008 465 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2149 2357 2925 3546 3557 3660 Size: 399 241 118 208 568 621 11 103 Cuts at: 3660 3668 3675 3930 3970 4252 4313 5055 5140 Size: 8 7 255 40 282 61 742 85 Cuts at: 5140 6211 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 621 568 399 282 255 241 208 118 103 85 61 40 11 8 7 BspEI T'CCGG_A Cuts at: 6657 6657 Size: 6994 BspHI T'CATG_A Cuts at: 4883 6613 4883 Size: 1730 5264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2371 3682 4349 844 Size: 1527 1311 667 3489 Fragments arranged by size: 3489 1527 1311 667 BsrBI CCG'CTC Cuts at: 1394 1823 4881 1394 Size: 429 3058 3507 Fragments arranged by size: 3507 3058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 3147 4008 5489 5663 65 Size: 1183 874 1025 861 1481 174 1396 Fragments arranged by size: 1481 1396 1183 1025 874 861 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2852 5638 5825 1700 Size: 110 1042 2786 187 2869 Fragments arranged by size: 2869 2786 1042 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6994 BssSI C'ACGA_G Cuts at: 2024 3737 5054 6066 2024 Size: 1713 1317 1012 2952 Fragments arranged by size: 2952 1713 1317 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3679 3688 794 Size: 2885 9 4100 Fragments arranged by size: 4100 2885 9 BstBI TT'CG_AA Cuts at: 5798 5798 Size: 6994 BstEII G'GTnAC_C Cuts at: 1184 3694 3797 1184 Size: 2510 103 4381 Fragments arranged by size: 4381 2510 103 BstXI CCAn_nnnn'nTGG Cuts at: 2579 3235 3449 4196 2579 Size: 656 214 747 5377 Fragments arranged by size: 5377 747 656 214 BstYI r'GATC_y Cuts at: 1972 3048 3340 3392 3589 4324 4691 5076 5093 Size: 1076 292 52 197 735 367 385 17 Cuts at: 5093 6545 6631 6643 6989 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1076 735 385 367 346 292 197 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 2650 3202 3477 3648 3864 4273 Size: 552 1575 10 552 275 171 216 409 Cuts at: 4273 513 Size: 3234 Fragments arranged by size: 3234 1575 552 552 409 275 216 171 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6255 Fragments arranged by size: 6255 683 56 BtsI GCAGTG_nn' Cuts at: 2438 4510 5304 5324 2438 Size: 2072 794 20 4108 Fragments arranged by size: 4108 2072 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2579 2614 2665 2700 529 Size: 35 2015 35 51 35 4823 Fragments arranged by size: 4823 2015 51 35 35 35 DraI TTT'AAA Cuts at: 4298 4298 Size: 6994 DraIII CAC_nnn'GTG Cuts at: 2731 2731 Size: 6994 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2288 6001 809 Size: 507 972 3713 1802 Fragments arranged by size: 3713 1802 972 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2167 2379 Size: 54 1425 50 275 18 210 127 212 Cuts at: 2379 3111 3225 3771 4441 5330 6959 8 Size: 732 114 546 670 889 1629 43 Fragments arranged by size: 1629 1425 889 732 670 546 275 212 210 127 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4441 1537 Size: 275 2629 4090 Fragments arranged by size: 4090 2629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4924 1353 Size: 3571 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5581 5955 6101 387 Size: 1064 138 3992 374 146 1280 Fragments arranged by size: 3992 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2855 2930 2956 3629 3682 3731 3858 Size: 393 1223 75 26 673 53 49 127 Cuts at: 3858 3862 5057 5654 6441 6709 1239 Size: 4 1195 597 787 268 1524 Fragments arranged by size: 1524 1223 1195 787 673 597 393 268 127 75 53 49 26 4 EcoNI CCTnn'n_nnAGG Cuts at: 3101 4232 3101 Size: 1131 5863 EcoO109I rG'GnC_Cy Cuts at: 2489 2761 2898 2963 3265 3313 3967 4200 2489 Size: 272 137 65 302 48 654 233 5283 Fragments arranged by size: 5283 654 302 272 233 137 65 48 EcoRI G'AATT_C Cuts at: 4303 4681 4303 Size: 378 6616 FseI GG_CCGG'CC Cuts at: 2856 2856 Size: 6994 FspI TGC'GCA Cuts at: 5500 5500 Size: 6994 HaeII r_GCGC'y Cuts at: 1678 2712 3576 3750 6141 1678 Size: 1034 864 174 2391 2531 Fragments arranged by size: 2531 2391 1034 864 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2629 2661 3332 3364 5635 Size: 32 135 32 1057 32 671 32 2271 Cuts at: 5635 5667 5709 5741 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2271 1057 671 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4213 4338 4584 669 Size: 988 281 2275 125 246 3079 Fragments arranged by size: 3079 2275 988 281 246 125 HindIII A'AGCT_T Cuts at: 748 748 Size: 6994 HpaI GTT'AAC Cuts at: 4584 4584 Size: 6994 KpnI G_GTAC'C Cuts at: 3895 4319 3895 Size: 424 6570 MfeI C'AATT_G Cuts at: 4593 4593 Size: 6994 MluI A'CGCG_T Cuts at: 6870 6870 Size: 6994 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4144 6108 6292 1305 Size: 2839 1964 184 2007 Fragments arranged by size: 2839 2007 1964 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 2381 3113 3227 3773 Size: 54 1425 343 337 212 732 114 546 Cuts at: 3773 10 Size: 3231 Fragments arranged by size: 3231 1425 732 546 343 337 212 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 3233 3447 3653 3821 4952 5311 5470 Size: 648 2067 214 206 168 1131 359 159 Cuts at: 5470 518 Size: 2042 Fragments arranged by size: 2067 2042 1131 648 359 214 206 168 159 MspA1I CmG'CkG Cuts at: 1606 2179 2236 2311 2778 2833 3679 3847 3882 Size: 573 57 75 467 55 846 168 35 Cuts at: 3882 3898 3913 4043 4410 5087 6235 6480 1606 Size: 16 15 130 367 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 677 573 467 367 245 168 130 75 57 55 35 16 15 NaeI GCC'GGC Cuts at: 2854 2854 Size: 6994 NcoI C'CATG_G Cuts at: 513 1065 3648 3864 513 Size: 552 2583 216 3643 Fragments arranged by size: 3643 2583 552 216 NdeI CA'TA_TG Cuts at: 387 387 Size: 6994 NgoMIV G'CCGG_C Cuts at: 2852 2852 Size: 6994 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6994 NotI GC'GGCC_GC Cuts at: 4441 4441 Size: 6994 NspI r_CATG'y Cuts at: 1132 1229 2461 4067 4352 5897 1132 Size: 97 1232 1606 285 1545 2229 Fragments arranged by size: 2229 1606 1545 1232 285 97 PasI CC'CwG_GG Cuts at: 1348 3550 1348 Size: 2202 4792 PciI A'CATG_T Cuts at: 5893 5893 Size: 6994 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2885 1108 Size: 1131 646 5217 Fragments arranged by size: 5217 1131 646 PmeI GTTT'AAAC Cuts at: 4298 4298 Size: 6994 PmlI CAC'GTG Cuts at: 2731 2731 Size: 6994 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5063 5095 6576 6608 5063 Size: 32 1481 32 5449 Fragments arranged by size: 5449 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2898 2898 Size: 6994 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6994 PsiI TTA'TAA Cuts at: 4564 4564 Size: 6994 PspOMI G'GGCC_C Cuts at: 3966 3966 Size: 6994 PstI C_TGCA'G Cuts at: 830 2329 3684 3693 4346 830 Size: 1499 1355 9 653 3478 Fragments arranged by size: 3478 1499 1355 653 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6994 PvuII CAG'CTG Cuts at: 2179 2311 2833 3679 3882 3898 4043 2179 Size: 132 522 846 203 16 145 5130 Fragments arranged by size: 5130 846 522 203 145 132 16 SalI G'TCGA_C Cuts at: 1936 4336 1936 Size: 2400 4594 SbfI CC_TGCA'GG Cuts at: 4346 4346 Size: 6994 ScaI AGT'ACT Cuts at: 5242 6954 5242 Size: 1712 5282 SexAI A'CCwGG_T Cuts at: 2625 2678 2625 Size: 53 6941 SfcI C'TryA_G Cuts at: 826 1045 2325 2945 3680 3689 4342 5477 6158 Size: 219 1280 620 735 9 653 1135 681 Cuts at: 6158 6349 826 Size: 191 1471 Fragments arranged by size: 1471 1280 1135 735 681 653 620 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6994 SmaI CCC'GGG Cuts at: 1872 2369 2928 3806 4321 1872 Size: 497 559 878 515 4545 Fragments arranged by size: 4545 878 559 515 497 SmlI C'TyrA_G Cuts at: 820 1017 1943 5098 5999 6261 820 Size: 197 926 3155 901 262 1553 Fragments arranged by size: 3155 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4785 493 Size: 4292 2702 SpeI A'CTAG_T Cuts at: 152 3079 152 Size: 2927 4067 SphI G_CATG'C Cuts at: 1132 4352 1132 Size: 3220 3774 SspI AAT'ATT Cuts at: 5 52 4918 5 Size: 47 4866 2081 Fragments arranged by size: 4866 2081 47 StuI AGG'CCT Cuts at: 1517 3107 1517 Size: 1590 5404 StyI C'CwwG_G Cuts at: 513 1065 2061 2153 2319 2959 3044 3067 3108 Size: 552 996 92 166 640 85 23 41 Cuts at: 3108 3648 3864 3962 4433 513 Size: 540 216 98 471 3074 Fragments arranged by size: 3074 996 640 552 540 471 216 166 98 92 85 41 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 4024 5021 5038 5191 5376 5821 Size: 1255 2001 443 997 17 153 185 445 Cuts at: 5821 325 Size: 1498 Fragments arranged by size: 2001 1498 1255 997 445 443 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3183 5240 6952 Size: 274 80 33 51 1443 1206 2057 1712 Cuts at: 6952 96 Size: 138 Fragments arranged by size: 2057 1712 1443 1206 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2976 3008 6954 6986 2976 Size: 32 3946 32 2984 Fragments arranged by size: 3946 2984 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6994 XbaI T'CTAG_A Cuts at: 4330 4330 Size: 6994 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2059 2059 Size: 6994 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6994 XmaI C'CCGG_G Cuts at: 1870 2367 2926 3804 4319 1870 Size: 497 559 878 515 4545 Fragments arranged by size: 4545 878 559 515 497 XmnI GAAnn'nnTTC Cuts at: 2795 5123 2795 Size: 2328 4666 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4802 276 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI