(Linear) (Six-base) MAPSORT of: KIEE5785.seq Check: 1580 from: 1 to: 2289 KIEE5785.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 366 1677 2289 Size: 366 1311 612 Fragments arranged by size: 1311 612 366 Acc65I G'GTAC_C Cuts at: 0 1886 2289 Size: 1886 403 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 925 951 1857 2289 Size: 925 26 906 432 Fragments arranged by size: 925 906 432 26 AleI CACnn'nnGTG Cuts at: 0 1228 1442 2289 Size: 1228 214 847 Fragments arranged by size: 1228 847 214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1834 2289 Size: 1834 455 AlwNI CAG_nnn'CTG Cuts at: 0 234 1683 2086 2289 Size: 234 1449 403 203 Fragments arranged by size: 1449 403 234 203 ApaI G_GGCC'C Cuts at: 0 1965 2289 Size: 1965 324 ApoI r'AATT_y Cuts at: 0 1122 2289 Size: 1122 1167 AvaI C'yCGr_G Cuts at: 0 362 699 921 1799 2289 Size: 362 337 222 878 490 Fragments arranged by size: 878 490 362 337 222 BanI G'GyrC_C Cuts at: 0 1886 2289 Size: 1886 403 BanII G_rGCy'C Cuts at: 0 352 1663 1925 1965 2289 Size: 352 1311 262 40 324 Fragments arranged by size: 1311 352 324 262 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 16 279 375 1425 2144 2289 Size: 16 263 96 1050 719 145 Fragments arranged by size: 1050 719 263 145 96 16 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2239 2273 2289 Size: 2239 34 16 Fragments arranged by size: 2239 34 16 BciVI GTATCCnnnnn_n' Cuts at: 0 1419 2289 Size: 1419 870 BglI GCCn_nnn'nGGC Cuts at: 0 367 2289 Size: 367 1922 BglII A'GATC_T Cuts at: 0 1335 1387 2289 Size: 1335 52 902 Fragments arranged by size: 1335 902 52 BlpI GC'TnA_GC Cuts at: 0 1822 2289 Size: 1822 467 Bme1580I G_kGCm'C Cuts at: 0 144 920 1552 1655 1965 2247 2289 Size: 144 776 632 103 310 282 42 Fragments arranged by size: 776 632 310 282 144 103 42 BmgBI CAC'GTC Cuts at: 0 462 2289 Size: 462 1827 BmrI ACTGGGnnnn_n' Cuts at: 0 131 831 1436 1520 1620 1919 2289 Size: 131 700 605 84 100 299 370 Fragments arranged by size: 700 605 370 299 131 100 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 1624 1677 1726 1853 2289 Size: 850 774 53 49 127 436 Fragments arranged by size: 850 774 436 127 53 49 Bpu10I CC'TnA_GC Cuts at: 0 187 1664 2289 Size: 187 1477 625 Fragments arranged by size: 1477 625 187 BsaI GGTCTCn'nnnn_ Cuts at: 0 1993 2289 Size: 1993 296 BsaAI yAC'GTr Cuts at: 0 726 1230 1276 2289 Size: 726 504 46 1013 Fragments arranged by size: 1013 726 504 46 BsaHI Gr'CG_yC Cuts at: 0 504 2289 Size: 504 1785 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 624 654 823 853 2289 Size: 624 30 169 30 1436 Fragments arranged by size: 1436 624 169 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 16 211 984 1729 1930 1933 2289 Size: 16 195 773 745 201 3 356 Fragments arranged by size: 773 745 356 201 195 16 3 BseYI C'CCAG_C Cuts at: 0 103 143 227 234 401 706 824 1128 Size: 103 40 84 7 167 305 118 304 Cuts at: 1128 1877 1904 2191 2289 Size: 749 27 287 98 Fragments arranged by size: 749 305 304 287 167 118 103 98 84 40 27 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 2289 Size: 703 1586 BsiHKAI G_wGCw'C Cuts at: 0 26 1541 1670 2289 Size: 26 1515 129 619 Fragments arranged by size: 1515 619 129 26 BsmI GAATG_Cn' Cuts at: 0 1027 1492 2289 Size: 1027 465 797 Fragments arranged by size: 1027 797 465 Bsp1286I G_dGCh'C Cuts at: 0 26 144 352 920 1541 1552 1655 1663 Size: 26 118 208 568 621 11 103 8 Cuts at: 1663 1670 1925 1965 2247 2289 Size: 7 255 40 282 42 Fragments arranged by size: 621 568 282 255 208 118 103 42 40 26 11 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 366 1677 2289 Size: 366 1311 612 Fragments arranged by size: 1311 612 366 BsrDI GCAATG_nn' Cuts at: 0 117 1142 2003 2289 Size: 117 1025 861 286 Fragments arranged by size: 1025 861 286 117 BsrFI r'CCGG_y Cuts at: 0 847 2289 Size: 847 1442 BssSI C'ACGA_G Cuts at: 0 19 1732 2289 Size: 19 1713 557 Fragments arranged by size: 1713 557 19 BstAPI GCAn_nnn'nTGC Cuts at: 0 1674 1683 2289 Size: 1674 9 606 Fragments arranged by size: 1674 606 9 BstEII G'GTnAC_C Cuts at: 0 1689 1792 2289 Size: 1689 103 497 Fragments arranged by size: 1689 497 103 BstXI CCAn_nnnn'nTGG Cuts at: 0 574 1230 1444 2191 2289 Size: 574 656 214 747 98 Fragments arranged by size: 747 656 574 214 98 BstYI r'GATC_y Cuts at: 0 1043 1335 1387 1584 2289 Size: 1043 292 52 197 705 Fragments arranged by size: 1043 705 292 197 52 BtgI C'CryG_G Cuts at: 0 635 645 1197 1472 1643 1859 2268 2289 Size: 635 10 552 275 171 216 409 21 Fragments arranged by size: 635 552 409 275 216 171 21 10 BtsI GCAGTG_nn' Cuts at: 0 433 2289 Size: 433 1856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 574 609 660 695 2289 Size: 574 35 51 35 1594 Fragments arranged by size: 1594 574 51 35 35 DraIII CAC_nnn'GTG Cuts at: 0 726 2289 Size: 726 1563 DrdI GACnn_nn'nnGTC Cuts at: 0 283 2289 Size: 283 2006 EaeI y'GGCC_r Cuts at: 0 35 162 374 1106 1220 1766 2289 Size: 35 127 212 732 114 546 523 Fragments arranged by size: 732 546 523 212 127 114 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 850 925 951 1624 1677 1726 1853 1857 Size: 850 75 26 673 53 49 127 4 Cuts at: 1857 2289 Size: 432 Fragments arranged by size: 850 673 432 127 75 53 49 26 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1096 2227 2289 Size: 1096 1131 62 Fragments arranged by size: 1131 1096 62 EcoO109I rG'GnC_Cy Cuts at: 0 484 756 893 958 1260 1308 1962 2195 Size: 484 272 137 65 302 48 654 233 Cuts at: 2195 2289 Size: 94 Fragments arranged by size: 654 484 302 272 233 137 94 65 48 FseI GG_CCGG'CC Cuts at: 0 851 2289 Size: 851 1438 HaeII r_GCGC'y Cuts at: 0 707 1571 1745 2289 Size: 707 864 174 544 Fragments arranged by size: 864 707 544 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 624 656 1327 1359 2289 Size: 624 32 671 32 930 Fragments arranged by size: 930 671 624 32 32 HincII GTy'rAC Cuts at: 0 2208 2289 Size: 2208 81 KpnI G_GTAC'C Cuts at: 0 1890 2289 Size: 1890 399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2139 2289 Size: 2139 150 MscI TGG'CCA Cuts at: 0 164 376 1108 1222 1768 2289 Size: 164 212 732 114 546 521 Fragments arranged by size: 732 546 521 212 164 114 MslI CAynn'nnrTG Cuts at: 0 1228 1442 1648 1816 2289 Size: 1228 214 206 168 473 Fragments arranged by size: 1228 473 214 206 168 MspA1I CmG'CkG Cuts at: 0 174 231 306 773 828 1674 1842 1877 Size: 174 57 75 467 55 846 168 35 Cuts at: 1877 1893 1908 2038 2289 Size: 16 15 130 251 Fragments arranged by size: 846 467 251 174 168 130 75 57 55 35 16 15 NaeI GCC'GGC Cuts at: 0 849 2289 Size: 849 1440 NcoI C'CATG_G Cuts at: 0 1643 1859 2289 Size: 1643 216 430 Fragments arranged by size: 1643 430 216 NgoMIV G'CCGG_C Cuts at: 0 847 2289 Size: 847 1442 NspI r_CATG'y Cuts at: 0 456 2062 2289 Size: 456 1606 227 Fragments arranged by size: 1606 456 227 PasI CC'CwG_GG Cuts at: 0 1545 2289 Size: 1545 744 PflMI CCAn_nnn'nTGG Cuts at: 0 234 880 2289 Size: 234 646 1409 Fragments arranged by size: 1409 646 234 PmlI CAC'GTG Cuts at: 0 726 2289 Size: 726 1563 PpuMI rG'GwC_Cy Cuts at: 0 893 2289 Size: 893 1396 PspOMI G'GGCC_C Cuts at: 0 1961 2289 Size: 1961 328 PstI C_TGCA'G Cuts at: 0 324 1679 1688 2289 Size: 324 1355 9 601 Fragments arranged by size: 1355 601 324 9 PvuII CAG'CTG Cuts at: 0 174 306 828 1674 1877 1893 2038 2289 Size: 174 132 522 846 203 16 145 251 Fragments arranged by size: 846 522 251 203 174 145 132 16 SexAI A'CCwGG_T Cuts at: 0 620 673 2289 Size: 620 53 1616 Fragments arranged by size: 1616 620 53 SfcI C'TryA_G Cuts at: 0 320 940 1675 1684 2289 Size: 320 620 735 9 605 Fragments arranged by size: 735 620 605 320 9 SmaI CCC'GGG Cuts at: 0 364 923 1801 2289 Size: 364 559 878 488 Fragments arranged by size: 878 559 488 364 SpeI A'CTAG_T Cuts at: 0 1074 2289 Size: 1074 1215 StuI AGG'CCT Cuts at: 0 1102 2289 Size: 1102 1187 StyI C'CwwG_G Cuts at: 0 56 148 314 954 1039 1062 1103 1643 Size: 56 92 166 640 85 23 41 540 Cuts at: 1643 1859 1957 2289 Size: 216 98 332 Fragments arranged by size: 640 540 332 216 166 98 92 85 56 41 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1576 2019 2289 Size: 1576 443 270 Fragments arranged by size: 1576 443 270 TatI w'GTAC_w Cuts at: 0 1178 2289 Size: 1178 1111 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 971 1003 2289 Size: 971 32 1286 Fragments arranged by size: 1286 971 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 54 2289 Size: 54 2235 XmaI C'CCGG_G Cuts at: 0 362 921 1799 2289 Size: 362 559 878 490 Fragments arranged by size: 878 559 490 362 XmnI GAAnn'nnTTC Cuts at: 0 790 2289 Size: 790 1499 Enzymes that do cut: AarI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraIII DrdI EaeI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PmlI PpuMI PspOMI PstI PvuII SexAI SfcI SmaI SpeI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BmtI BplI BpuEI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI DraI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI