Restriction map for
pFN21AE2016
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BamHI BbsI BcgI
BciVI BclI BfrI BpuAI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MfeI MluI MroI MseI MunI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 40 GTCTAC GT^[AC][GT]AC
AcsI 844 GAATTC [GA]^AATT[CT]
ApoI 844 GAATTC [GA]^AATT[CT]
AspI 1072 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1362 CCTAGG C^CTAGG
BbrPI 1672 CACGTG CAC^GTG
BbvCI 1468 GCTGAGG CCTCAGC(-5/-2)
BglI 224 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 774 AGATCT A^GATCT
BlnI 1362 CCTAGG C^CTAGG
BsaAI 1672 CACGTG [CT]AC^GT[GA]
BsaBI 972 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 98 TCCGGT [AT]^CCGG[AT]
BsiEI 100 CGGTCG CG[GA][CT]^CG
BsmI 1751 GCATTC GAATGC(1/-1)
BspLU11I 1153 ACATGT A^CATGT
BsrBI 1644 GAGCGG CCGCTC(-3/-3)
BstXI 1332 CCATAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 735 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 844 GAATTC G^AATTC
HincII 429 GTTGAC GT[CT]^[GA]AC
HindII 429 GTTGAC GT[CT]^[GA]AC
KspI 1613 CCGCGG CCGC^GG
MamI 972 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1130 CATATG CA^TATG
NspI 1153 ACATGT [GA]CATG^[CT]
PflFI 1072 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1761 GACTC GAGTC(4/5)
PmaCI 1672 CACGTG CAC^GTG
PmlI 1672 CACGTG CAC^GTG
PshAI 1548 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1613 CCGCGG CCGC^GG
SexAI 1529 ACCTGGT A^CC[AT]GGT
SmlI 994 CTTGAG C^T[CT][GA]AG
StuI 1310 AGGCCT AGG^CCT
TaqI 1775 TCGA T^CGA
Tsp45I 1250 GTGAC ^GT[GC]AC
Tth111I 1072 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1775 TCGA T^CGA
XcmI 441 CCAGACCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
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AatII 313 GACGTC GACGT^C
331 GACGTC
AfaI 462 GTAC GT^AC
1023 GTAC
AflIII 825 ACGTGT A^C[GA][CT]GT
1153 ACATGT
AlwI 1029 GATCC GGATC(4/5)
1455 GGATC
AvaI 29 CCCGAG C^[CT]CG[GA]G
1442 CCCGAG
BalI 974 TGGCCA TGG^CCA
1420 TGGCCA
BanI 34 GGCGCC G^G[CT][GA]CC
307 GGCGCC
BbeI 34 GGCGCC GGCGC^C
307 GGCGCC
BsgI 694 CTGCAC GTGCAG(16/14)
259 GTGCAG
BsmFI 54 GTCCC GGGAC(10/14)
589 GTCCC
BsoBI 29 CCCGAG C^[CT]CG[GA]G
1442 CCCGAG
BspMI 206 ACCTGC ACCTGC(4/8)
1115 ACCTGC
BstAPI 480 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
642 GCAGCCGGTGC
BstYI 774 AGATCT [GA]^GATC[CT]
1028 AGATCC
EcoT14I 10 CCAAGG C^C[AT][AT]GG
1362 CCTAGG
HgaI 123 GACGC GACGC(5/10)
1580 GACGC
KasI 34 GGCGCC G^GCGCC
307 GGCGCC
MflI 774 AGATCT [GA]^GATC[CT]
1028 AGATCC
MluNI 974 TGGCCA TGG^CCA
1420 TGGCCA
MscI 974 TGGCCA TGG^CCA
1420 TGGCCA
NaeI 1516 GCCGGC GCC^GGC
1625 GCCGGC
NarI 34 GGCGCC GG^CGCC
307 GGCGCC
NgoMIV 1516 GCCGGC G^CCGGC
1625 GCCGGC
PflMI 441 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1296 CCAGCCTGTGG
PpuMI 1043 AGGACCT [GA]G^G[AT]CC[CT]
1358 AGGTCCT
PstI 208 CTGCAG CTGCA^G
1630 CTGCAG
RsaI 462 GTAC GT^AC
1023 GTAC
SacI 1144 GAGCTC GAGCT^C
1200 GAGCTC
SapI 783 GAAGAGC GCTCTTC(1/4)
1197 GAAGAGC
SfcI 208 CTGCAG C^T[GA][CT]AG
1630 CTGCAG
SfoI 34 GGCGCC GGC^GCC
307 GGCGCC
StyI 10 CCAAGG C^C[AT][AT]GG
1362 CCTAGG
Van91I 441 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1296 CCAGCCTGTGG
XhoII 774 AGATCT [GA]^GATC[CT]
1028 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaII BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp509I TspRI