(Circular) (Six-base) MAPSORT of: pFN21AE2016.seq Check: 5483 from: 1 to: 6484 pFN21AE2016 6484 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2220 2220 Size: 6484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2322 2340 4294 278 Size: 53 83 186 936 786 18 1954 2468 Fragments arranged by size: 2468 1954 936 786 186 83 53 18 AccI GT'mk_AC Cuts at: 1937 2046 3827 1937 Size: 109 1781 4594 Fragments arranged by size: 4594 1781 109 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3065 3245 3422 4547 5931 6199 1632 Size: 1433 180 177 1125 1384 268 1917 Fragments arranged by size: 1917 1433 1384 1125 268 180 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2830 3158 5383 6360 1203 Size: 1627 328 2225 977 1327 Fragments arranged by size: 2225 1627 1327 977 328 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2322 1184 Size: 1138 5346 AlwNI CAG_nnn'CTG Cuts at: 2452 3043 3223 3535 3572 4227 5799 2452 Size: 591 180 312 37 655 1572 3137 Fragments arranged by size: 3137 1572 655 591 312 180 37 ApaI G_GGCC'C Cuts at: 2240 2916 3742 2240 Size: 676 826 4982 Fragments arranged by size: 4982 826 676 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 2849 3793 4019 4171 6280 1493 Size: 1356 944 226 152 2109 1697 Fragments arranged by size: 2109 1697 1356 944 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2034 3447 3809 1870 Size: 73 91 1413 362 4545 Fragments arranged by size: 4545 1413 362 91 73 AvrII C'CTAG_G Cuts at: 3367 3367 Size: 6484 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2312 3805 5260 618 Size: 325 217 627 252 273 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 273 252 217 BanII G_rGCy'C Cuts at: 1391 2240 2586 2916 3153 3209 3742 3803 1391 Size: 849 346 330 237 56 533 61 4072 Fragments arranged by size: 4072 849 533 346 330 237 61 56 BbeI G_GCGC'C Cuts at: 2043 2316 2043 Size: 273 6211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5513 BbvCI CC'TCA_GC Cuts at: 3474 3474 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2235 5095 136 Size: 107 122 71 631 605 563 2860 1525 Fragments arranged by size: 2860 1525 631 605 563 122 107 71 BglII A'GATC_T Cuts at: 2779 6479 2779 Size: 3700 2784 BlpI GC'TnA_GC Cuts at: 2244 3216 3686 3901 2244 Size: 972 470 215 4827 Fragments arranged by size: 4827 972 470 215 Bme1580I G_kGCm'C Cuts at: 1790 2240 2577 2916 3742 4545 5701 1790 Size: 450 337 339 826 803 1156 2573 Fragments arranged by size: 2573 1156 826 803 450 339 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 4215 5173 Size: 416 53 83 399 24 828 1970 958 Cuts at: 5173 442 Size: 1753 Fragments arranged by size: 1970 1753 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2957 3032 3076 5144 1239 Size: 1013 705 75 44 2068 2579 Fragments arranged by size: 2579 2068 1013 705 75 44 Bpu10I CC'TnA_GC Cuts at: 2805 3474 2805 Size: 669 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3020 4609 5474 5772 3020 Size: 1589 865 298 3732 Fragments arranged by size: 3732 1589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 2643 2954 5147 882 Size: 617 618 526 311 2193 2219 Fragments arranged by size: 2219 2193 618 617 526 311 BsaAI yAC'GTr Cuts at: 493 1204 3679 4275 493 Size: 711 2475 596 2702 Fragments arranged by size: 2702 2475 711 596 BsaBI GATnn'nnATC Cuts at: 2981 6478 2981 Size: 3497 2987 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2129 2313 2319 Size: 53 83 186 936 507 89 184 6 Cuts at: 2319 2337 4291 4673 5308 275 Size: 18 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 507 382 186 184 89 83 53 18 6 BsaWI w'CCGG_w Cuts at: 2103 4917 5315 5589 5736 6147 2103 Size: 2814 398 274 147 411 2440 Fragments arranged by size: 2814 2440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3135 3165 1178 Size: 30 165 30 1732 30 4497 Fragments arranged by size: 4497 1732 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2972 3079 3467 1186 Size: 1786 107 388 4203 Fragments arranged by size: 4203 1786 388 107 BseYI C'CCAG_C Cuts at: 5687 6183 5687 Size: 496 5988 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2684 1163 Size: 573 549 399 4963 Fragments arranged by size: 4963 573 549 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2108 3934 4695 5723 1540 Size: 275 186 107 1826 761 1028 2301 Fragments arranged by size: 2301 1826 1028 761 275 186 107 BsiHKAI G_wGCw'C Cuts at: 2029 2375 3153 3209 3687 4545 4630 5701 2029 Size: 346 778 56 478 858 85 1071 2812 Fragments arranged by size: 2812 1071 858 778 478 346 85 56 BsmI GAATG_Cn' Cuts at: 3756 3995 4088 3756 Size: 239 93 6152 Fragments arranged by size: 6152 239 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2240 2375 2577 2586 2916 3153 Size: 399 239 211 135 202 9 330 237 Cuts at: 3153 3209 3687 3742 3803 4545 4630 5701 1391 Size: 56 478 55 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 330 239 237 211 202 135 85 61 56 55 9 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3129 3839 844 Size: 1376 909 710 3489 Fragments arranged by size: 3489 1376 909 710 BsrBI CCG'CTC Cuts at: 1394 1823 3651 4371 1394 Size: 429 1828 720 3507 Fragments arranged by size: 3507 1828 720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4979 5153 65 Size: 1183 3731 174 1396 Fragments arranged by size: 3731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2650 3521 3630 5128 5315 1700 Size: 110 840 871 109 1498 187 2869 Fragments arranged by size: 2869 1498 871 840 187 110 109 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 2491 2653 794 Size: 1697 162 4625 Fragments arranged by size: 4625 1697 162 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 3344 3344 Size: 6484 BstYI r'GATC_y Cuts at: 1972 2779 3033 3814 4181 4566 4583 6035 6121 Size: 807 254 781 367 385 17 1452 86 Cuts at: 6121 6133 6479 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 807 781 385 367 346 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2577 2628 2668 3618 513 Size: 552 1512 51 40 950 3379 Fragments arranged by size: 3379 1512 950 552 51 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 4000 4794 4814 4000 Size: 794 20 5670 Fragments arranged by size: 5670 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 2716 3682 3696 2716 Size: 966 14 5504 Fragments arranged by size: 5504 966 14 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2631 2979 Size: 54 1425 50 275 18 264 537 348 Cuts at: 2979 3425 3931 4820 6449 8 Size: 446 506 889 1629 43 Fragments arranged by size: 1629 1425 889 537 506 446 348 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2783 3066 3197 4414 1353 Size: 1430 283 131 1217 3423 Fragments arranged by size: 3423 1430 1217 283 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2984 3089 5071 5445 5591 387 Size: 1064 138 1395 105 1982 374 146 1280 Fragments arranged by size: 1982 1395 1280 1064 374 146 138 105 EcoICRI GAG'CTC Cuts at: 3151 3207 3151 Size: 56 6428 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2957 3032 3065 3076 3245 3422 Size: 393 620 705 75 33 11 169 177 Cuts at: 3422 4547 5144 5931 6199 1239 Size: 1125 597 787 268 1524 Fragments arranged by size: 1524 1125 787 705 620 597 393 268 177 169 75 33 11 EcoNI CCTnn'n_nnAGG Cuts at: 2744 2744 Size: 6484 EcoO109I rG'GnC_Cy Cuts at: 2053 2236 3049 3229 3364 3739 3775 2053 Size: 183 813 180 135 375 36 4762 Fragments arranged by size: 4762 813 375 183 180 135 36 EcoRI G'AATT_C Cuts at: 2849 3793 4171 2849 Size: 944 378 5162 Fragments arranged by size: 5162 944 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2392 2890 2392 Size: 498 5986 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2043 2316 2676 5631 1678 Size: 365 273 360 2955 2531 Fragments arranged by size: 2955 2531 365 360 273 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 3135 3167 5125 Size: 32 135 32 456 32 1075 32 1958 Cuts at: 5125 5157 5199 5231 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1958 1075 456 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2436 3828 4074 669 Size: 988 281 498 1392 246 3079 Fragments arranged by size: 3079 1392 988 498 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KasI G'GCGC_C Cuts at: 2039 2312 2039 Size: 273 6211 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2839 5598 5782 1305 Size: 1534 2759 184 2007 Fragments arranged by size: 2759 2007 1534 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2981 3427 10 Size: 54 1425 343 1149 446 3067 Fragments arranged by size: 3067 1425 1149 446 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2304 2844 3342 4442 4801 4960 518 Size: 648 1138 540 498 1100 359 159 2042 Fragments arranged by size: 2042 1138 1100 648 540 498 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2508 2517 2918 3040 3400 3506 3620 Size: 528 374 9 401 122 360 106 114 Cuts at: 3620 3900 4577 5725 5970 1606 Size: 280 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 528 401 374 360 280 245 122 114 106 9 NaeI GCC'GGC Cuts at: 3523 3632 3523 Size: 109 6375 NarI GG'CG_CC Cuts at: 2040 2313 2040 Size: 273 6211 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5932 NdeI CA'TA_TG Cuts at: 387 3136 387 Size: 2749 3735 NgoMIV G'CCGG_C Cuts at: 3521 3630 3521 Size: 109 6375 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 3162 3842 5387 1132 Size: 97 1933 680 1545 2229 Fragments arranged by size: 2229 1933 1545 680 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 3158 5383 3158 Size: 2225 4259 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 3307 1108 Size: 1344 855 4285 Fragments arranged by size: 4285 1344 855 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PmlI CAC'GTG Cuts at: 3679 3679 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3049 3364 3049 Size: 315 6169 PshAI GACnn'nnGTC Cuts at: 1613 3557 1613 Size: 1944 4540 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2236 2912 3738 2236 Size: 676 826 4982 Fragments arranged by size: 4982 826 676 PstI C_TGCA'G Cuts at: 830 2217 3639 3836 830 Size: 1387 1422 197 3478 Fragments arranged by size: 3478 1422 1387 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2508 2517 3040 3400 2508 Size: 9 523 360 5592 Fragments arranged by size: 5592 523 360 9 SacI G_AGCT'C Cuts at: 3153 3209 3153 Size: 56 6428 SacII CC_GC'GG Cuts at: 3621 3621 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SapI GCTCTTCn'nnn_ Cuts at: 2783 3197 2783 Size: 414 6070 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SexAI A'CCwGG_T Cuts at: 3534 3534 Size: 6484 SfcI C'TryA_G Cuts at: 826 1045 2213 3635 3832 4967 5648 5839 826 Size: 219 1168 1422 197 1135 681 191 1471 Fragments arranged by size: 1471 1422 1168 1135 681 219 197 191 SfoI GGC'GCC Cuts at: 2041 2314 2041 Size: 273 6211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2999 4588 5489 5751 820 Size: 197 926 1056 1589 901 262 1553 Fragments arranged by size: 1589 1553 1056 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 3317 1517 Size: 1800 4684 StyI C'CwwG_G Cuts at: 513 1065 2015 3367 3923 513 Size: 552 950 1352 556 3074 Fragments arranged by size: 3074 1352 950 556 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3728 4511 4528 4681 4866 5311 325 Size: 1255 2148 783 17 153 185 445 1498 Fragments arranged by size: 2148 1498 1255 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 4730 6442 Size: 274 80 33 51 1443 489 2264 1712 Cuts at: 6442 96 Size: 138 Fragments arranged by size: 2264 1712 1443 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3407 3439 3471 3503 6444 6476 3407 Size: 32 32 32 2941 32 3415 Fragments arranged by size: 3415 2941 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3080 1316 Size: 1764 4720 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2453 2453 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2320 2338 4292 276 Size: 53 83 186 936 786 18 1954 2468 Fragments arranged by size: 2468 1954 936 786 186 83 53 18 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SanDI SfiI SrfI SwaI