################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BstBI CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MfeI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 754 AAATTT [GA]^AATT[CT] Alw44I 169 GTGCAC G^TGCAC ApaI 1196 GGGCCC GGGCC^C ApaLI 169 GTGCAC G^TGCAC ApoI 754 AAATTT [GA]^AATT[CT] BglI 1015 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 922 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1498 GAGACG CGTCTC(1/5) BspHI 434 TCATGA T^CATGA BsrFI 974 GCCGGC [GA]^CCGG[CT] BssSI 1311 CACGAG CACGAG(-5/-1) Bst1107I 1432 GTATAC GTA^TAC BstEII 177 GGTGACC G^GT[ACGT]ACC BstPI 177 GGTGACC G^GT[ACGT]ACC BstXI 1310 CCACGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1432 GTATAC GTA^TAC Cfr10I 974 GCCGGC [GA]^CCGG[CT] EarI 193 GAAGAG CTCTTC(1/4) EcoNI 1704 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 177 GGTGACC G^GT[ACGT]ACC HincII 160 GTCAAC GT[CT]^[GA]AC HindII 160 GTCAAC GT[CT]^[GA]AC HinfI 1041 GAATC G^A[ACGT]TC Ksp632I 193 GAAGAG CTCTTC(1/4) MamI 922 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 1436 ACGCGT A^CGCGT MseI 455 TTAA T^TAA NaeI 974 GCCGGC GCC^GGC NgoMIV 974 GCCGGC G^CCGGC RcaI 434 TCATGA T^CATGA SacI 361 GAGCTC GAGCT^C SexAI 173 ACCTGGT A^CC[AT]GGT TfiI 1041 GAATC G^A[AT]TC Tru9I 455 TTAA T^TAA Tsp509I 755 AATT ^AATT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 421 GTCTAC GT^[AC][GT]AC 1432 GTATAC AflIII 578 ACACGT A^C[GA][CT]GT 1436 ACGCGT AocI 849 CCTGAGG CC^T[ACGT]AGG 1927 CCTCAGG AspI 415 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 883 GACACCGTC BclI 29 TGATCA T^GATCA 1481 TGATCA BfaI 124 CTAG C^TAG 448 CTAG BsaI 1921 GAGACC GGTCTC(1/5) 493 GGTCTC BsrDI 1345 CATTGC GCAATG(2/0) 1349 GCAATG Bsu36I 849 CCTGAGG CC^T[ACGT]AGG 1927 CCTCAGG DraII 520 GGGGCCT [GA]G^G[ACGT]CC[CT] 1172 AGGGCCT DraIII 172 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 579 CACGTGGTG Eco81I 849 CCTGAGG CC^T[ACGT]AGG 1927 CCTCAGG EcoO109I 520 GGGGCCT [GA]G^G[ACGT]CC[CT] 1172 AGGGCCT FbaI 29 TGATCA T^GATCA 1481 TGATCA MaeI 124 CTAG C^TAG 448 CTAG NcoI 1391 CCATGG C^CATGG 1692 CCATGG NspI 206 GCATGC [GA]CATG^[CT] 1910 GCATGC PflFI 415 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 883 GACACCGTC PvuII 616 CAGCTG CAG^CTG 1830 CAGCTG SmaI 92 CCCGGG CCC^GGG 1415 CCCGGG SphI 206 GCATGC GCATG^C 1910 GCATGC Tth111I 415 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC 883 GACACCGTC XmaCI 92 CCCGGG C^CCGGG 1415 CCCGGG XmaI 92 CCCGGG C^CCGGG 1415 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvI BcnI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PmaCI PmlI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII