(Linear) (Six-base) MAPSORT of: KIEE5742.seq Check: 2106 from: 1 to: 2034 KIEE5742.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 771 2034 Size: 771 1263 AccI GT'mk_AC Cuts at: 0 422 1433 2034 Size: 422 1011 601 Fragments arranged by size: 1011 601 422 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 805 1472 1489 2034 Size: 718 87 667 17 545 Fragments arranged by size: 718 667 545 87 17 AflIII A'CryG_T Cuts at: 0 578 1436 2034 Size: 578 858 598 Fragments arranged by size: 858 598 578 AleI CACnn'nnGTG Cuts at: 0 1315 2034 Size: 1315 719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 581 1720 1930 2018 2034 Size: 581 1139 210 88 16 Fragments arranged by size: 1139 581 210 88 16 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 443 475 2034 Size: 443 32 1559 Fragments arranged by size: 1559 443 32 AlwNI CAG_nnn'CTG Cuts at: 0 489 514 960 1176 1206 1522 1841 2034 Size: 489 25 446 216 30 316 319 193 Fragments arranged by size: 489 446 319 316 216 193 30 25 ApaI G_GGCC'C Cuts at: 0 1200 2034 Size: 1200 834 ApaLI G'TGCA_C Cuts at: 0 169 2034 Size: 169 1865 ApoI r'AATT_y Cuts at: 0 754 2034 Size: 754 1280 AvaI C'yCGr_G Cuts at: 0 92 340 1103 1415 2034 Size: 92 248 763 312 619 Fragments arranged by size: 763 619 312 248 92 BamHI G'GATC_C Cuts at: 0 678 1776 1937 2034 Size: 678 1098 161 97 Fragments arranged by size: 1098 678 161 97 BanI G'GyrC_C Cuts at: 0 79 119 284 1720 2034 Size: 79 40 165 1436 314 Fragments arranged by size: 1436 314 165 79 40 BanII G_rGCy'C Cuts at: 0 365 1200 1373 2034 Size: 365 835 173 661 Fragments arranged by size: 835 661 365 173 BbsI GAAGACnn'nnnn_ Cuts at: 0 94 363 1791 2034 Size: 94 269 1428 243 Fragments arranged by size: 1428 269 243 94 BclI T'GATC_A Cuts at: 0 29 1481 2034 Size: 29 1452 553 Fragments arranged by size: 1452 553 29 BglI GCCn_nnn'nGGC Cuts at: 0 1021 2034 Size: 1021 1013 Bme1580I G_kGCm'C Cuts at: 0 173 471 1200 1416 1723 1967 2034 Size: 173 298 729 216 307 244 67 Fragments arranged by size: 729 307 298 244 216 173 67 BmgBI CAC'GTC Cuts at: 0 552 942 2034 Size: 552 390 1092 Fragments arranged by size: 1092 552 390 BmrI ACTGGGnnnn_n' Cuts at: 0 1636 2034 Size: 1636 398 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 445 751 1072 1144 1516 1755 1939 Size: 64 381 306 321 72 372 239 184 Cuts at: 1939 2034 Size: 95 Fragments arranged by size: 381 372 321 306 239 184 95 72 64 BsaI GGTCTCn'nnnn_ Cuts at: 0 499 1915 2034 Size: 499 1416 119 Fragments arranged by size: 1416 499 119 BsaAI yAC'GTr Cuts at: 0 259 357 581 1653 2034 Size: 259 98 224 1072 381 Fragments arranged by size: 1072 381 259 224 98 BsaBI GATnn'nnATC Cuts at: 0 926 2034 Size: 926 1108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 131 161 443 473 1130 1160 2034 Size: 25 106 30 282 30 657 30 874 Fragments arranged by size: 874 657 282 106 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 358 361 364 1339 2034 Size: 358 3 3 975 695 Fragments arranged by size: 975 695 358 3 3 BseYI C'CCAG_C Cuts at: 0 182 489 614 814 1199 1216 1613 2034 Size: 182 307 125 200 385 17 397 421 Fragments arranged by size: 421 397 385 307 200 182 125 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 288 1549 1675 2034 Size: 288 1261 126 359 Fragments arranged by size: 1261 359 288 126 BsiHKAI G_wGCw'C Cuts at: 0 173 356 365 629 941 1237 1652 1898 Size: 173 183 9 264 312 296 415 246 Cuts at: 1898 2034 Size: 136 Fragments arranged by size: 415 312 296 264 246 183 173 136 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1492 2034 Size: 1492 542 Bsp1286I G_dGCh'C Cuts at: 0 173 356 365 471 629 941 1200 1237 Size: 173 183 9 106 158 312 259 37 Cuts at: 1237 1373 1416 1652 1723 1898 1967 2034 Size: 136 43 236 71 175 69 67 Fragments arranged by size: 312 259 236 183 175 173 158 136 106 71 69 67 43 37 9 BspHI T'CATG_A Cuts at: 0 434 2034 Size: 434 1600 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 302 771 1712 2034 Size: 302 469 941 322 Fragments arranged by size: 941 469 322 302 BsrDI GCAATG_nn' Cuts at: 0 1344 1356 2034 Size: 1344 12 678 Fragments arranged by size: 1344 678 12 BsrFI r'CCGG_y Cuts at: 0 974 2034 Size: 974 1060 BssSI C'ACGA_G Cuts at: 0 1311 2034 Size: 1311 723 BstAPI GCAn_nnn'nTGC Cuts at: 0 1176 1522 1673 2034 Size: 1176 346 151 361 Fragments arranged by size: 1176 361 346 151 BstEII G'GTnAC_C Cuts at: 0 177 2034 Size: 177 1857 BstXI CCAn_nnnn'nTGG Cuts at: 0 1317 2034 Size: 1317 717 BstYI r'GATC_y Cuts at: 0 678 956 1067 1562 1569 1776 1937 2034 Size: 678 278 111 495 7 207 161 97 Fragments arranged by size: 678 495 278 207 161 111 97 7 BstZ17I GTA'TAC Cuts at: 0 1434 2034 Size: 1434 600 Bsu36I CC'TnA_GG Cuts at: 0 850 1928 2034 Size: 850 1078 106 Fragments arranged by size: 1078 850 106 BtgI C'CryG_G Cuts at: 0 74 896 1391 1692 2034 Size: 74 822 495 301 342 Fragments arranged by size: 822 495 342 301 74 BtsI GCAGTG_nn' Cuts at: 0 1523 2034 Size: 1523 511 DraIII CAC_nnn'GTG Cuts at: 0 177 584 2034 Size: 177 407 1450 Fragments arranged by size: 1450 407 177 EaeI y'GGCC_r Cuts at: 0 110 270 1307 2034 Size: 110 160 1037 727 Fragments arranged by size: 1037 727 160 110 EarI CTCTTCn'nnn_ Cuts at: 0 188 2034 Size: 188 1846 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1336 1950 2034 Size: 1336 614 84 Fragments arranged by size: 1336 614 84 EcoICRI GAG'CTC Cuts at: 0 363 2034 Size: 363 1671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 445 718 751 805 1072 1144 1472 Size: 64 381 273 33 54 267 72 328 Cuts at: 1472 1489 1516 1755 1939 2034 Size: 17 27 239 184 95 Fragments arranged by size: 381 328 273 267 239 184 95 72 64 54 33 27 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 1708 2034 Size: 1708 326 EcoO109I rG'GnC_Cy Cuts at: 0 521 1173 2034 Size: 521 652 861 Fragments arranged by size: 861 652 521 HaeII r_GCGC'y Cuts at: 0 858 975 1495 2034 Size: 858 117 520 539 Fragments arranged by size: 858 539 520 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 27 1554 1586 1999 2031 2034 Size: 27 1527 32 413 32 3 Fragments arranged by size: 1527 413 32 32 27 3 HincII GTy'rAC Cuts at: 0 162 2034 Size: 162 1872 MluI A'CGCG_T Cuts at: 0 1436 2034 Size: 1436 598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1997 2034 Size: 1997 37 MscI TGG'CCA Cuts at: 0 112 272 1309 2034 Size: 112 160 1037 725 Fragments arranged by size: 1037 725 160 112 MslI CAynn'nnrTG Cuts at: 0 1240 1315 1349 1733 2034 Size: 1240 75 34 384 301 Fragments arranged by size: 1240 384 301 75 34 MspA1I CmG'CkG Cuts at: 0 618 1617 1629 1832 2034 Size: 618 999 12 203 202 Fragments arranged by size: 999 618 203 202 12 NaeI GCC'GGC Cuts at: 0 976 2034 Size: 976 1058 NcoI C'CATG_G Cuts at: 0 1391 1692 2034 Size: 1391 301 342 Fragments arranged by size: 1391 342 301 NgoMIV G'CCGG_C Cuts at: 0 974 2034 Size: 974 1060 NspI r_CATG'y Cuts at: 0 210 1914 2034 Size: 210 1704 120 Fragments arranged by size: 1704 210 120 PflMI CCAn_nnn'nTGG Cuts at: 0 262 489 514 1952 2034 Size: 262 227 25 1438 82 Fragments arranged by size: 1438 262 227 82 25 PfoI T'CCnGG_A Cuts at: 0 1565 2034 Size: 1565 469 PmlI CAC'GTG Cuts at: 0 259 357 581 1653 2034 Size: 259 98 224 1072 381 Fragments arranged by size: 1072 381 259 224 98 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 443 475 2034 Size: 443 32 1559 Fragments arranged by size: 1559 443 32 PspOMI G'GGCC_C Cuts at: 0 1196 2034 Size: 1196 838 PstI C_TGCA'G Cuts at: 0 323 560 623 920 1181 1577 1837 1865 Size: 323 237 63 297 261 396 260 28 Cuts at: 1865 1997 2034 Size: 132 37 Fragments arranged by size: 396 323 297 261 260 237 132 63 37 28 PvuII CAG'CTG Cuts at: 0 618 1832 2034 Size: 618 1214 202 Fragments arranged by size: 1214 618 202 SacI G_AGCT'C Cuts at: 0 365 2034 Size: 365 1669 SexAI A'CCwGG_T Cuts at: 0 173 2034 Size: 173 1861 SfcI C'TryA_G Cuts at: 0 319 556 619 916 1177 1573 1833 1861 Size: 319 237 63 297 261 396 260 28 Cuts at: 1861 1993 2034 Size: 132 41 Fragments arranged by size: 396 319 297 261 260 237 132 63 41 28 SmaI CCC'GGG Cuts at: 0 94 1417 2034 Size: 94 1323 617 Fragments arranged by size: 1323 617 94 SphI G_CATG'C Cuts at: 0 210 1914 2034 Size: 210 1704 120 Fragments arranged by size: 1704 210 120 SrfI GCCC'GGGC Cuts at: 0 1417 2034 Size: 1417 617 StyI C'CwwG_G Cuts at: 0 143 905 1391 1692 2034 Size: 143 762 486 301 342 Fragments arranged by size: 762 486 342 301 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1252 1738 2034 Size: 1252 486 296 Fragments arranged by size: 1252 486 296 TatI w'GTAC_w Cuts at: 0 650 2034 Size: 650 1384 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 131 163 568 600 1130 1162 2034 Size: 131 32 405 32 530 32 872 Fragments arranged by size: 872 530 405 131 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 418 886 2034 Size: 418 468 1148 Fragments arranged by size: 1148 468 418 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 682 1207 1779 2034 Size: 682 525 572 255 Fragments arranged by size: 682 572 525 255 XmaI C'CCGG_G Cuts at: 0 92 1415 2034 Size: 92 1323 619 Fragments arranged by size: 1323 619 92 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI BglI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PpiI PspOMI PstI PvuII SacI SexAI SfcI SmaI SphI SrfI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrBI BsrGI BssHII BstBI BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SspI StuI SwaI XbaI XhoI XmnI ZraI