Restriction map for
pFN21AE4202
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BbvCI BciVI BfrI BglII
BlnI BsaBI BsaI BseAI BseRI
BsiWI BspDI BspEI BspHI BspMI
BssHII BstBI BstEII BstPI BstXI
Bsu36I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI HincII
HindII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 263 GGCGCC G[GA]^CG[CT]C
Alw44I 517 GTGCAC G^TGCAC
AlwNI 55 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 517 GTGCAC G^TGCAC
AviII 419 TGCGCA TGC^GCA
BamHI 1979 GGATCC G^GATCC
BbeI 263 GGCGCC GGCGC^C
BbrPI 1972 CACGTG CAC^GTG
BcgI 1191 CGATGGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1079 TGATCA T^GATCA
BfaI 1489 CTAG C^TAG
BglI 91 GCCGGCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 629 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 629 GCTTAGC GC^T[ACGT]AGC
BsaHI 263 GGCGCC G[GA]^CG[CT]C
BsgI 216 GTGCAG GTGCAG(16/14)
BsmBI 79 GAGACG CGTCTC(1/5)
BssSI 520 CACGAG CACGAG(-5/-1)
Bst1107I 1510 GTATAC GTA^TAC
BstAPI 519 GCACGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1510 GTATAC GTA^TAC
CelII 629 GCTTAGC GC^T[ACGT]AGC
DsaI 38 CCGCGG C^C[GA][CT]GG
FbaI 1079 TGATCA T^GATCA
FspI 419 TGCGCA TGC^GCA
HaeII 263 GGCGCC [GA]GCGC^[CT]
Hin1I 263 GGCGCC G[GA]^CG[CT]C
HindIII 1770 AAGCTT A^AGCTT
HphI 1185 TCACC GGTGA(8/7)
KasI 263 GGCGCC G^GCGCC
KspI 38 CCGCGG CCGC^GG
MaeI 1489 CTAG C^TAG
NaeI 91 GCCGGC GCC^GGC
NarI 263 GGCGCC GG^CGCC
NgoMIV 91 GCCGGC G^CCGGC
NotI 34 GCGGCCGC GC^GGCCGC
PmaCI 1972 CACGTG CAC^GTG
PmlI 1972 CACGTG CAC^GTG
SacI 429 GAGCTC GAGCT^C
SacII 38 CCGCGG CCGC^GG
ScaI 359 AGTACT AGT^ACT
SfoI 263 GGCGCC GGC^GCC
SmlI 586 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1510 GTATAC GT^[AC][GT]AC
1534 GTAGAC
AgeI 1502 ACCGGT A^CCGGT
1988 ACCGGT
Asp700I 787 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1325 GAAAGCATTC
AspHI 429 GAGCTC G[AT]GC[AT]^C
517 GTGCAC
AvaI 378 CTCGGG C^[CT]CG[GA]G
402 CTCGGG
AvaII 117 GGTCC G^G[AT]CC
2046 GGACC
BanI 263 GGCGCC G^G[CT][GA]CC
1458 GGCACC
BbsI 1498 GAAGAC GAAGAC(2/6)
1831 GTCTTC
BpuAI 1498 GAAGAC GAAGAC(2/6)
1831 GTCTTC
BsaAI 1222 TACGTG [CT]AC^GT[GA]
1972 CACGTG
BsaWI 1502 ACCGGT [AT]^CCGG[AT]
1988 ACCGGT
BsiEI 35 CGGCCG CG[GA][CT]^CG
83 CGGCCG
BsiHKAI 429 GAGCTC G[AT]GC[AT]^C
517 GTGCAC
BsmI 2119 GAATGC GAATGC(1/-1)
1329 GCATTC
BsoBI 378 CTCGGG C^[CT]CG[GA]G
402 CTCGGG
Bsp1407I 482 TGTACA T^GTACA
641 TGTACA
BsrDI 1515 CATTGC GCAATG(2/0)
166 GCAATG
BsrGI 482 TGTACA T^GTACA
641 TGTACA
DraII 269 AGGGCCT [GA]G^G[ACGT]CC[CT]
441 GGGGCCC
DraIII 520 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
697 CACAAAGTG
EagI 35 CGGCCG C^GGCCG
83 CGGCCG
EclXI 35 CGGCCG C^GGCCG
83 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
83 CGGCCG
EcoO109I 269 AGGGCCT [GA]G^G[ACGT]CC[CT]
441 GGGGCCC
HgaI 970 GACGC GACGC(5/10)
500 GCGTC
MslI 1071 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1186 CACCTCGATG
PinAI 1502 ACCGGT A^CCGGT
1988 ACCGGT
PleI 1590 GACTC GAGTC(4/5)
394 GAGTC
SspBI 482 TGTACA T^GTACA
641 TGTACA
XmnI 787 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1325 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII