Restriction map for
pFN21AE4202
(Circular) (Six-base) MAPSORT of: pFN21AE4202.seq Check: 5270 from: 1 to: 6829
pFN21AE4202 6829 bp
With 182 enzymes: SgfI *
March 28, 2012 13:06 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3516 3540 4172 1937
Size: 1579 24 632 4594
Fragments arranged by size:
4594 1579 632 24
Acc65I G'GTAC_C
Cuts at: 4150 4150
Size: 6829
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2690 2811 2859 3573 4892 6276 6544
Size: 410 648 121 48 714 1319 1384 268
Cuts at: 6544 1632
Size: 1917
Fragments arranged by size:
1917 1384 1319 714 648 410 268 121
48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 2484 2643 2917 3228 3307 5728 6705 1203
Size: 1281 159 274 311 79 2421 977 1327
Fragments arranged by size:
2421 1327 1281 977 311 274 159 79
AgeI A'CCGG_T
Cuts at: 3507 3993 5660 3507
Size: 486 1667 4676
Fragments arranged by size:
4676 1667 486
AhdI GACnn_n'nnGTC
Cuts at: 5558 5558
Size: 6829
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3832 1184
Size: 2648 4181
AlwNI CAG_nnn'CTG
Cuts at: 2065 4572 6144 2065
Size: 2507 1572 2750
Fragments arranged by size:
2750 2507 1572
ApaI G_GGCC'C
Cuts at: 2201 2335 2451 2201
Size: 134 116 6579
Fragments arranged by size:
6579 134 116
ApaLI G'TGCA_C
Cuts at: 2522 4886 6042 2522
Size: 2364 1156 3309
Fragments arranged by size:
3309 2364 1156
ApoI r'AATT_y
Cuts at: 1493 2573 2585 2695 2723 3289 4138 4364 4516
Size: 1080 12 110 28 566 849 226 152
Cuts at: 4516 6625 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1080 849 566 226 152 110
28 12
AseI AT'TA_AT
Cuts at: 160 1032 5383 160
Size: 872 4351 1606
Fragments arranged by size:
4351 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 2383 2407 4154 1870
Size: 73 440 24 1747 4545
Fragments arranged by size:
4545 1747 440 73 24
BamHI G'GATC_C
Cuts at: 3984 4159 4526 3984
Size: 175 367 6287
Fragments arranged by size:
6287 367 175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 3463 4150 5605 618
Size: 325 217 627 481 1195 687 1455 1842
Fragments arranged by size:
1842 1455 1195 687 627 481 325 217
BanII G_rGCy'C
Cuts at: 1391 2201 2335 2438 2451 4148 1391
Size: 810 134 103 13 1697 4072
Fragments arranged by size:
4072 1697 810 134 103 13
BbeI G_GCGC'C
Cuts at: 2272 2272
Size: 6829
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3510 3829 928
Size: 971 1611 319 3928
Fragments arranged by size:
3928 1611 971 319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3185 3219 5020 5054 1903
Size: 34 1248 34 1801 34 3678
Fragments arranged by size:
3678 1801 1248 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5931 4721
Size: 1210 5619
BclI T'GATC_A
Cuts at: 1548 3084 1548
Size: 1536 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2102 5440
Size: 107 122 71 631 605 337 93 3338
Cuts at: 5440 136
Size: 1525
Fragments arranged by size:
3338 1525 631 605 337 122 107 93
71
BglII A'GATC_T
Cuts at: 6824 6824
Size: 6829
BlpI GC'TnA_GC
Cuts at: 2635 4246 2635
Size: 1611 5218
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2335 2451 2526 4890 6046 1790
Size: 411 134 116 75 2364 1156 2573
Fragments arranged by size:
2573 2364 1156 411 134 116 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4560 5518 442
Size: 416 53 83 399 24 3143 958 1753
Fragments arranged by size:
3143 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2422 2606 3009 2242
Size: 180 184 403 6062
Fragments arranged by size:
6062 403 184 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2334 2555 3768 5489 1239
Size: 848 247 221 1213 1721 2579
Fragments arranged by size:
2579 1721 1213 848 247 221
Bpu10I CC'TnA_GC
Cuts at: 2654 2879 3432 3794 2654
Size: 225 553 362 5689
Fragments arranged by size:
5689 553 362 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 4954 5819 6117 2576
Size: 2378 865 298 3288
Fragments arranged by size:
3288 2378 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5492 882
Size: 617 3993 2219
Fragments arranged by size:
3993 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3229 3979 4620 493
Size: 711 2025 750 641 2702
Fragments arranged by size:
2702 2025 750 711 641
BsaBI GATnn'nnATC
Cuts at: 6823 6823
Size: 6829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2269 4636 5018 5653
Size: 53 83 186 936 736 2367 382 635
Cuts at: 5653 275
Size: 1451
Fragments arranged by size:
2367 1451 936 736 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3507 3993 5262 5660 5934 6081 6492 3507
Size: 486 1269 398 274 147 411 3844
Fragments arranged by size:
3844 1269 486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3242 3272 1178
Size: 30 165 30 1839 30 4735
Fragments arranged by size:
4735 1839 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6829
BseYI C'CCAG_C
Cuts at: 6032 6528 6032
Size: 496 6333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2242 1163
Size: 573 506 5750
Fragments arranged by size:
5750 573 506
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 2091 4279 5040 6068 1540
Size: 275 186 42 48 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186 48 42
BsiHKAI G_wGCw'C
Cuts at: 2438 2526 4890 4975 6046 2438
Size: 88 2364 85 1071 3221
Fragments arranged by size:
3221 2364 1071 88 85
BsmI GAATG_Cn'
Cuts at: 3334 4130 4340 4433 3334
Size: 796 210 93 5730
Fragments arranged by size:
5730 796 210 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2078 2078
Size: 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2335 2438 2451 2526 4148 4890
Size: 399 411 134 103 13 75 1622 742
Cuts at: 4890 4975 6046 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1622 1071 742 411 399 134 103
85 75 13
BspEI T'CCGG_A
Cuts at: 6492 6492
Size: 6829
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4184 844
Size: 3340 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2184 2340 2406 4716 1394
Size: 429 361 156 66 2310 3507
Fragments arranged by size:
3507 2310 429 361 156 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 2178 3519 5324 5498 65
Size: 1183 930 1341 1805 174 1396
Fragments arranged by size:
1805 1396 1341 1183 930 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 3507 3993 5473 5660 1700
Size: 110 286 1411 486 1480 187 2869
Fragments arranged by size:
2869 1480 1411 486 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 2646 96
Size: 2391 159 4279
Fragments arranged by size:
4279 2391 159
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 2525 4889 5901 2525
Size: 2364 1012 3453
Fragments arranged by size:
3453 2364 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2530 794
Size: 1736 5093
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstYI r'GATC_y
Cuts at: 1972 2163 3765 3984 4159 4526 4911 4928 6380
Size: 191 1602 219 175 367 385 17 1452
Cuts at: 6380 6466 6478 6824 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1602 1452 385 367 346 219 191
175 86 17 12
BstZ17I GTA'TAC
Cuts at: 3517 3517
Size: 6829
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 513
Size: 552 939 39 5299
Fragments arranged by size:
5299 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6090
Fragments arranged by size:
6090 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 2230 2985 3966 4345 5139 5159 2025
Size: 205 755 981 379 794 20 3695
Fragments arranged by size:
3695 981 794 755 379 205 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6829
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2601 2636 529
Size: 35 2037 35 4722
Fragments arranged by size:
4722 2037 35 35
DraI TTT'AAA
Cuts at: 2568 2872 3409 4133 2568
Size: 304 537 724 5264
Fragments arranged by size:
5264 724 537 304
DraIII CAC_nnn'GTG
Cuts at: 2530 2707 2530
Size: 177 6652
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5836 809
Size: 507 4520 1802
Fragments arranged by size:
4520 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2088 2112
Size: 54 1425 50 275 18 210 48 24
Cuts at: 2112 2541 4276 5165 6794 8
Size: 429 1735 889 1629 43
Fragments arranged by size:
1735 1629 1425 889 429 275 210 54
50 48 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 2088 4276 1537
Size: 275 228 48 2188 4090
Fragments arranged by size:
4090 2188 275 228 48
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2285 2787 2835 3720 3914 4759 1353
Size: 708 224 502 48 885 194 845 3423
Fragments arranged by size:
3423 885 845 708 502 224 194 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5416 5790 5936 387
Size: 1064 138 3827 374 146 1280
Fragments arranged by size:
3827 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2436 2436
Size: 6829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2087 2334 2555 2690 2811 2859
Size: 393 410 45 247 221 135 121 48
Cuts at: 2859 3573 3768 4892 5489 6276 6544 1239
Size: 714 195 1124 597 787 268 1524
Fragments arranged by size:
1524 1124 787 714 597 410 393 268
247 221 195 135 121 48 45
EcoO109I rG'GnC_Cy
Cuts at: 2275 2447 2275
Size: 172 6657
EcoRI G'AATT_C
Cuts at: 4138 4516 4138
Size: 378 6451
FspI TGC'GCA
Cuts at: 2426 5335 2426
Size: 2909 3920
HaeII r_GCGC'y
Cuts at: 1678 2272 5976 1678
Size: 594 3704 2531
Fragments arranged by size:
3704 2531 594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3602 3634 4066 4098 5470
Size: 32 135 32 2030 32 432 32 1372
Cuts at: 5470 5502 5544 5576 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2030 1372 432 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4173 4419 669
Size: 988 281 2235 246 3079
Fragments arranged by size:
3079 2235 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3775 748
Size: 3027 3802
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KasI G'GCGC_C
Cuts at: 2268 2268
Size: 6829
KpnI G_GTAC'C
Cuts at: 4154 4154
Size: 6829
MfeI C'AATT_G
Cuts at: 4428 4428
Size: 6829
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5943 6127 1305
Size: 4638 184 2007
Fragments arranged by size:
4638 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5007
Fragments arranged by size:
5007 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3080 3195 4787 5146 5305 518
Size: 648 1914 115 1592 359 159 2042
Fragments arranged by size:
2042 1914 1592 648 359 159 115
MspA1I CmG'CkG
Cuts at: 1606 2045 2133 2266 4245 4922 6070 6315 1606
Size: 439 88 133 1979 677 1148 245 2120
Fragments arranged by size:
2120 1979 1148 677 439 245 133 88
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 6829
NarI GG'CG_CC
Cuts at: 2269 2269
Size: 6829
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5338
Fragments arranged by size:
5338 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6829
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 6829
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6829
NotI GC'GGCC_GC
Cuts at: 2040 4276 2040
Size: 2236 4593
NspI r_CATG'y
Cuts at: 1132 1229 2488 2647 2921 2993 3027 3206 3311
Size: 97 1259 159 274 72 34 179 105
Cuts at: 3311 4187 5732 1132
Size: 876 1545 2229
Fragments arranged by size:
2229 1545 1259 876 274 179 159 105
97 72 34
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6829
PciI A'CATG_T
Cuts at: 2484 2643 2917 3307 5728 2484
Size: 159 274 390 2421 3585
Fragments arranged by size:
3585 2421 390 274 159
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6829
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PmlI CAC'GTG
Cuts at: 3979 3979
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 3426 4399 3426
Size: 973 5856
PspOMI G'GGCC_C
Cuts at: 2197 2331 2447 2197
Size: 134 116 6579
Fragments arranged by size:
6579 134 116
PstI C_TGCA'G
Cuts at: 830 4181 830
Size: 3351 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
SacI G_AGCT'C
Cuts at: 2438 2438
Size: 6829
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 6829
SalI G'TCGA_C
Cuts at: 1936 4171 1936
Size: 2235 4594
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 2366 5077 6789 2366
Size: 2711 1712 2406
Fragments arranged by size:
2711 2406 1712
SfcI C'TryA_G
Cuts at: 826 1045 3270 3370 3537 4177 5312 5993 6184
Size: 219 2225 100 167 640 1135 681 191
Cuts at: 6184 826
Size: 1471
Fragments arranged by size:
2225 1471 1135 681 640 219 191 167
100
SfoI GGC'GCC
Cuts at: 2270 2270
Size: 6829
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 4156 1872
Size: 2284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 4933 5834 6096 820
Size: 197 926 648 2342 901 262 1553
Fragments arranged by size:
2342 1553 926 901 648 262 197
SnaBI TAC'GTA
Cuts at: 493 4620 493
Size: 4127 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6829
SphI G_CATG'C
Cuts at: 1132 4187 1132
Size: 3055 3774
SspI AAT'ATT
Cuts at: 5 52 4753 5
Size: 47 4701 2081
Fragments arranged by size:
4701 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6829
StyI C'CwwG_G
Cuts at: 513 1065 2004 4268 513
Size: 552 939 2264 3074
Fragments arranged by size:
3074 2264 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4856 4873 5026 5211 5656 325
Size: 1255 3276 17 153 185 445 1498
Fragments arranged by size:
3276 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2364 2481 2487
Size: 274 80 33 51 1443 387 117 6
Cuts at: 2487 2646 5075 6787 96
Size: 159 2429 1712 138
Fragments arranged by size:
2429 1712 1443 387 274 159 138 117
80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 3847 3879 6789 6821 2204
Size: 32 1611 32 2910 32 2212
Fragments arranged by size:
2910 2212 1611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6829
XmaI C'CCGG_G
Cuts at: 1870 4154 1870
Size: 2284 4545
XmnI GAAnn'nnTTC
Cuts at: 2796 3334 4958 2796
Size: 538 1624 4667
Fragments arranged by size:
4667 1624 538
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstXI Bsu36I EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI