(Linear) (Six-base) MAPSORT of: KIEE4202.seq Check: 8486 from: 1 to: 2124 KIEE4202.orf With 182 enzymes: SgfI * March 22, 2012 17:34 .. AccI GT'mk_AC Cuts at: 0 1511 1535 2124 Size: 1511 24 589 Fragments arranged by size: 1511 589 24 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 685 806 854 1568 2124 Size: 37 648 121 48 714 556 Fragments arranged by size: 714 648 556 121 48 37 AflIII A'CryG_T Cuts at: 0 479 638 912 1223 1302 2124 Size: 479 159 274 311 79 822 Fragments arranged by size: 822 479 311 274 159 79 AgeI A'CCGG_T Cuts at: 0 1502 1988 2124 Size: 1502 486 136 Fragments arranged by size: 1502 486 136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1827 2124 Size: 1827 297 AlwNI CAG_nnn'CTG Cuts at: 0 60 2124 Size: 60 2064 ApaI G_GGCC'C Cuts at: 0 196 330 446 2124 Size: 196 134 116 1678 Fragments arranged by size: 1678 196 134 116 ApaLI G'TGCA_C Cuts at: 0 517 2124 Size: 517 1607 ApoI r'AATT_y Cuts at: 0 568 580 690 718 1284 2124 Size: 568 12 110 28 566 840 Fragments arranged by size: 840 568 566 110 28 12 AvaI C'yCGr_G Cuts at: 0 378 402 2124 Size: 378 24 1722 Fragments arranged by size: 1722 378 24 BamHI G'GATC_C Cuts at: 0 1979 2124 Size: 1979 145 BanI G'GyrC_C Cuts at: 0 263 1458 2124 Size: 263 1195 666 Fragments arranged by size: 1195 666 263 BanII G_rGCy'C Cuts at: 0 196 330 433 446 2124 Size: 196 134 103 13 1678 Fragments arranged by size: 1678 196 134 103 13 BbeI G_GCGC'C Cuts at: 0 267 2124 Size: 267 1857 BbsI GAAGACnn'nnnn_ Cuts at: 0 1505 1824 2124 Size: 1505 319 300 Fragments arranged by size: 1505 319 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1180 1214 2124 Size: 1180 34 910 Fragments arranged by size: 1180 910 34 BclI T'GATC_A Cuts at: 0 1079 2124 Size: 1079 1045 BglI GCCn_nnn'nGGC Cuts at: 0 97 2124 Size: 97 2027 BlpI GC'TnA_GC Cuts at: 0 630 2124 Size: 630 1494 Bme1580I G_kGCm'C Cuts at: 0 196 330 446 521 2124 Size: 196 134 116 75 1603 Fragments arranged by size: 1603 196 134 116 75 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 237 417 601 1004 2124 Size: 237 180 184 403 1120 Fragments arranged by size: 1120 403 237 184 180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 329 550 1763 2124 Size: 82 247 221 1213 361 Fragments arranged by size: 1213 361 247 221 82 Bpu10I CC'TnA_GC Cuts at: 0 649 874 1427 1789 2124 Size: 649 225 553 362 335 Fragments arranged by size: 649 553 362 335 225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 2124 Size: 571 1553 BsaAI yAC'GTr Cuts at: 0 1224 1974 2124 Size: 1224 750 150 Fragments arranged by size: 1224 750 150 BsaHI Gr'CG_yC Cuts at: 0 264 2124 Size: 264 1860 BsaWI w'CCGG_w Cuts at: 0 1502 1988 2124 Size: 1502 486 136 Fragments arranged by size: 1502 486 136 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1237 1267 2124 Size: 1237 30 857 Fragments arranged by size: 1237 857 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 237 2124 Size: 237 1887 BsiEI CG_ry'CG Cuts at: 0 38 86 2124 Size: 38 48 2038 Fragments arranged by size: 2038 48 38 BsiHKAI G_wGCw'C Cuts at: 0 433 521 2124 Size: 433 88 1603 Fragments arranged by size: 1603 433 88 BsmI GAATG_Cn' Cuts at: 0 1329 2124 Size: 1329 795 BsmBI CGTCTCn'nnnn_ Cuts at: 0 73 2124 Size: 73 2051 Bsp1286I G_dGCh'C Cuts at: 0 196 330 433 446 521 2124 Size: 196 134 103 13 75 1603 Fragments arranged by size: 1603 196 134 103 75 13 BsrBI CCG'CTC Cuts at: 0 179 335 401 2124 Size: 179 156 66 1723 Fragments arranged by size: 1723 179 156 66 BsrDI GCAATG_nn' Cuts at: 0 173 1514 2124 Size: 173 1341 610 Fragments arranged by size: 1341 610 173 BsrFI r'CCGG_y Cuts at: 0 91 1502 1988 2124 Size: 91 1411 486 136 Fragments arranged by size: 1411 486 136 91 BsrGI T'GTAC_A Cuts at: 0 482 641 2124 Size: 482 159 1483 Fragments arranged by size: 1483 482 159 BssSI C'ACGA_G Cuts at: 0 520 2124 Size: 520 1604 BstAPI GCAn_nnn'nTGC Cuts at: 0 525 2124 Size: 525 1599 BstYI r'GATC_y Cuts at: 0 158 1760 1979 2124 Size: 158 1602 219 145 Fragments arranged by size: 1602 219 158 145 BstZ17I GTA'TAC Cuts at: 0 1512 2124 Size: 1512 612 BtgI C'CryG_G Cuts at: 0 38 2124 Size: 38 2086 BtsI GCAGTG_nn' Cuts at: 0 20 225 980 1961 2124 Size: 20 205 755 981 163 Fragments arranged by size: 981 755 205 163 20 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 596 631 2124 Size: 596 35 1493 Fragments arranged by size: 1493 596 35 DraI TTT'AAA Cuts at: 0 563 867 1404 2124 Size: 563 304 537 720 Fragments arranged by size: 720 563 537 304 DraIII CAC_nnn'GTG Cuts at: 0 525 702 2124 Size: 525 177 1422 Fragments arranged by size: 1422 525 177 EaeI y'GGCC_r Cuts at: 0 35 83 107 536 2124 Size: 35 48 24 429 1588 Fragments arranged by size: 1588 429 48 35 24 EagI C'GGCC_G Cuts at: 0 35 83 2124 Size: 35 48 2041 Fragments arranged by size: 2041 48 35 EarI CTCTTCn'nnn_ Cuts at: 0 56 280 782 830 1715 1909 2124 Size: 56 224 502 48 885 194 215 Fragments arranged by size: 885 502 224 215 194 56 48 EcoICRI GAG'CTC Cuts at: 0 431 2124 Size: 431 1693 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 82 329 550 685 806 854 1568 Size: 37 45 247 221 135 121 48 714 Cuts at: 1568 1763 2124 Size: 195 361 Fragments arranged by size: 714 361 247 221 195 135 121 48 45 37 EcoO109I rG'GnC_Cy Cuts at: 0 270 442 2124 Size: 270 172 1682 Fragments arranged by size: 1682 270 172 FspI TGC'GCA Cuts at: 0 421 2124 Size: 421 1703 HaeII r_GCGC'y Cuts at: 0 267 2124 Size: 267 1857 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1597 1629 2061 2093 2124 Size: 1597 32 432 32 31 Fragments arranged by size: 1597 432 32 32 31 HindIII A'AGCT_T Cuts at: 0 1770 2124 Size: 1770 354 KasI G'GCGC_C Cuts at: 0 263 2124 Size: 263 1861 MslI CAynn'nnrTG Cuts at: 0 1075 1190 2124 Size: 1075 115 934 Fragments arranged by size: 1075 934 115 MspA1I CmG'CkG Cuts at: 0 40 128 261 2124 Size: 40 88 133 1863 Fragments arranged by size: 1863 133 88 40 NaeI GCC'GGC Cuts at: 0 93 2124 Size: 93 2031 NarI GG'CG_CC Cuts at: 0 264 2124 Size: 264 1860 NgoMIV G'CCGG_C Cuts at: 0 91 2124 Size: 91 2033 NotI GC'GGCC_GC Cuts at: 0 35 2124 Size: 35 2089 NspI r_CATG'y Cuts at: 0 483 642 916 988 1022 1201 1306 2124 Size: 483 159 274 72 34 179 105 818 Fragments arranged by size: 818 483 274 179 159 105 72 34 PciI A'CATG_T Cuts at: 0 479 638 912 1302 2124 Size: 479 159 274 390 822 Fragments arranged by size: 822 479 390 274 159 PmlI CAC'GTG Cuts at: 0 1974 2124 Size: 1974 150 PsiI TTA'TAA Cuts at: 0 1421 2124 Size: 1421 703 PspOMI G'GGCC_C Cuts at: 0 192 326 442 2124 Size: 192 134 116 1682 Fragments arranged by size: 1682 192 134 116 SacI G_AGCT'C Cuts at: 0 433 2124 Size: 433 1691 SacII CC_GC'GG Cuts at: 0 41 2124 Size: 41 2083 ScaI AGT'ACT Cuts at: 0 361 2124 Size: 361 1763 SfcI C'TryA_G Cuts at: 0 1265 1365 1532 2124 Size: 1265 100 167 592 Fragments arranged by size: 1265 592 167 100 SfoI GGC'GCC Cuts at: 0 265 2124 Size: 265 1859 SmlI C'TyrA_G Cuts at: 0 586 2124 Size: 586 1538 TatI w'GTAC_w Cuts at: 0 359 476 482 641 2124 Size: 359 117 6 159 1483 Fragments arranged by size: 1483 359 159 117 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 199 231 1842 1874 2124 Size: 199 32 1611 32 250 Fragments arranged by size: 1611 250 199 32 32 XmnI GAAnn'nnTTC Cuts at: 0 791 1329 2124 Size: 791 538 795 Fragments arranged by size: 795 791 538 Enzymes that do cut: AccI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstYI BstZ17I BtgI BtsI CspCI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HindIII KasI MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI PmlI PsiI PspOMI SacI SacII ScaI SfcI SfoI SmlI TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BfrBI BglII BmgBI BmrI BmtI BsaI BsaBI BseRI BseYI BsiWI BspEI BspHI BspMI BssHII BstBI BstEII BstXI Bsu36I BtgZI ClaI DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NruI NsiI PacI PasI PflMI PfoI PmeI PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI